BRENDA - Enzyme Database

Identification, cloning, and expression of bacteriophage T5 dnk gene encoding a broad specificity deoxyribonucleoside monophosphate kinase (EC 2.7.4.13)

Mikoulinskaia, G.V.; Zimin, A.A.; Feofanov, S.A.; Miroshnikov, A.I.; Protein Expr. Purif. 33, 166-175 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.13
expression in Escherichia coli
Escherichia virus T5
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.13
28670
-
calculated from nucleotid sequence
Escherichia virus T5
2.7.4.13
29140
-
SDS-PAGE
Escherichia virus T5
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside monophosphate
Escherichia virus T5
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.13
Escherichia virus T5
Q6QGP4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
2.7.4.13
two alternative methods, the first involves ion-exchange chromatography on Mono Q, the second involves affinity chromatography on Toyopearl AF-Red, the protein is purified 5.34fold in both cases
Escherichia virus T5
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.4.13
29
-
recombinant protein in sonicated cell exctracts, with dGMP as substrate
Escherichia virus T5
2.7.4.13
36
-
recombinant protein after Toyopearl 650 M flow-through, with dGMP as substrate
Escherichia virus T5
2.7.4.13
40
-
recombinant protein after ammonium sulphate precipitation, with dGMP as substrate
Escherichia virus T5
2.7.4.13
84.2
-
homogeneous recombinant enzyme with dCMP as substrate
Escherichia virus T5
2.7.4.13
153.6
-
homogeneous recombinant enzyme with dGMP as substrate
Escherichia virus T5
2.7.4.13
155
-
affinity chromatography eluant of the recombinant protein, with dGMP as substrate; ion-exchange chromatography MonoQ eluant of the recombinant protein, with dGMP as substrate
Escherichia virus T5
2.7.4.13
166.8
-
homogeneous recombinant enzyme with dTMP as substrate
Escherichia virus T5
2.7.4.13
232
-
homogeneous recombinant enzyme with dAMP as substrate
Escherichia virus T5
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + dAMP
-
663294
Escherichia virus T5
ADP + dADP
-
-
-
?
2.7.4.13
ATP + dCMP
-
663294
Escherichia virus T5
ADP + dCDP
-
-
-
?
2.7.4.13
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
663294
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
-
-
?
2.7.4.13
ATP + dGMP
-
663294
Escherichia virus T5
ADP + dGDP
-
-
-
?
2.7.4.13
ATP + dTMP
-
663294
Escherichia virus T5
ADP + dTDP
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.13
expression in Escherichia coli
Escherichia virus T5
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.13
28670
-
calculated from nucleotid sequence
Escherichia virus T5
2.7.4.13
29140
-
SDS-PAGE
Escherichia virus T5
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside monophosphate
Escherichia virus T5
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.13
two alternative methods, the first involves ion-exchange chromatography on Mono Q, the second involves affinity chromatography on Toyopearl AF-Red, the protein is purified 5.34fold in both cases
Escherichia virus T5
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
2.7.4.13
29
-
recombinant protein in sonicated cell exctracts, with dGMP as substrate
Escherichia virus T5
2.7.4.13
36
-
recombinant protein after Toyopearl 650 M flow-through, with dGMP as substrate
Escherichia virus T5
2.7.4.13
40
-
recombinant protein after ammonium sulphate precipitation, with dGMP as substrate
Escherichia virus T5
2.7.4.13
84.2
-
homogeneous recombinant enzyme with dCMP as substrate
Escherichia virus T5
2.7.4.13
153.6
-
homogeneous recombinant enzyme with dGMP as substrate
Escherichia virus T5
2.7.4.13
155
-
affinity chromatography eluant of the recombinant protein, with dGMP as substrate; ion-exchange chromatography MonoQ eluant of the recombinant protein, with dGMP as substrate
Escherichia virus T5
2.7.4.13
166.8
-
homogeneous recombinant enzyme with dTMP as substrate
Escherichia virus T5
2.7.4.13
232
-
homogeneous recombinant enzyme with dAMP as substrate
Escherichia virus T5
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + dAMP
-
663294
Escherichia virus T5
ADP + dADP
-
-
-
?
2.7.4.13
ATP + dCMP
-
663294
Escherichia virus T5
ADP + dCDP
-
-
-
?
2.7.4.13
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
663294
Escherichia virus T5
ADP + deoxynucleoside diphosphate
-
-
-
?
2.7.4.13
ATP + dGMP
-
663294
Escherichia virus T5
ADP + dGDP
-
-
-
?
2.7.4.13
ATP + dTMP
-
663294
Escherichia virus T5
ADP + dTDP
-
-
-
?