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Literature summary extracted from
- Computationally designed variants of Escherichia coli chorismate mutase show altered catalytic activity (2005), Protein Eng. Des. Sel., 18, 161-163.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | expression in Escherichia coli BL21(DE3) | Escherichia coli |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | - |
Escherichia coli |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | A32S | increased catalytic efficiency | Escherichia coli |
| 5.4.99.5 | D48I | no activity | Escherichia coli |
| 5.4.99.5 | I81L/V85I | reduced catalytic efficiency, alters packing against the hydrophobic ring face of the reacting molecule | Escherichia coli |
| 5.4.99.5 | L7I | no significant effect on catalytic efficiency | Escherichia coli |
| 5.4.99.5 | V35I | increased KM and turnover values compared to wild type | Escherichia coli |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.22 | - |
chorismate | mutant A32S | Escherichia coli |  |
| 5.4.99.5 | 0.249 | - |
chorismate | mutant L7I | Escherichia coli | |
| 5.4.99.5 | 0.304 | - |
chorismate | wild type | Escherichia coli | |
| 5.4.99.5 | 0.365 | - |
chorismate | mutant V35I | Escherichia coli | |
| 5.4.99.5 | 0.669 | - |
chorismate | mutant I81L/V85I | Escherichia coli | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | Escherichia coli | - |
Prephenate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | Escherichia coli | P0A9J8 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | - |
Escherichia coli |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | - |
Escherichia coli | Prephenate | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | chorismate mutase | - |
Escherichia coli |
| 5.4.99.5 | EcCM | - |
Escherichia coli |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 33.4 | - |
chorismate | mutant I81L/V85I | Escherichia coli | |
| 5.4.99.5 | 36.55 | - |
chorismate | mutant L7I | Escherichia coli | |
| 5.4.99.5 | 38.87 | - |
chorismate | wild type | Escherichia coli | |
| 5.4.99.5 | 45.13 | - |
chorismate | mutant A32S | Escherichia coli | |
| 5.4.99.5 | 50.77 | - |
chorismate | mutant V35I | Escherichia coli | |
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Release 2023.1 (January 2023)
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