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Literature summary extracted from

  • Reinbothe, C.; Bartsch, S.; Eggink, L.L.; Hoober, J.K.; Brusslan, J.; Andrade-Paz, R.; Monnet, J.; Reinbothe, S.
    A role for chlorophyllide a oxygenase in the regulated import and stabilization of light-harvesting chlorophyll a/b proteins (2006), Proc. Natl. Acad. Sci. USA, 103, 4777-4782.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.122 expression of His6-tagged CAO in Escherichia coli in inclusion bodies, in vitro expression by wheat germ lysates Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.14.13.122 chloroplast intrinsic in inner envelope and thylakoid membrane Arabidopsis thaliana 9507
-
1.14.13.122 additional information the enzyme is part of a separate translocon complex in chlorplasts Arabidopsis thaliana
-
-
1.14.13.122 thylakoid membrane
-
 Arabidopsis thaliana 42651
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.122 Fe2+ the enzyme contains a [2Fe-2S] Rieske cluster Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.122 additional information Arabidopsis thaliana the enzyme is part of a separate translocon complex involved in the regulated import and stabilization of the chlorophyllide b-binding light-harvesting proteins Lhcb1, of LHCII, and Lhcb4, of CP29, in chlorplasts ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.122 Arabidopsis thaliana
-
 gene CAO
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.122 refolded recombinant His6-tagged CAO from Escherichia coli by nickel affinity chromatography to about 85% purity Arabidopsis thaliana

Renatured (Commentary)

EC Number Renatured (Comment) Organism
1.14.13.122 solubilization of recombinant His6-tagged enzyme from inclusion bodies after expression in Escherichia coli by 8 M urea and refolding Arabidopsis thaliana

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.14.13.122 additional information
-
 the assay is performed in darkness Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.122 7-hydroxychlorophyllide a + O2 + NADPH
-
 Arabidopsis thaliana chlorophyllide b + H2O + NADP+
-
 ?
1.14.13.122 chlorophyllide a + O2 + NADPH + H+
-
 Arabidopsis thaliana 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?
1.14.13.122 additional information the enzyme is part of a separate translocon complex involved in the regulated import and stabilization of the chlorophyllide b-binding light-harvesting proteins Lhcb1, of LHCII, and Lhcb4, of CP29, in chlorplasts Arabidopsis thaliana ?
-
 ?
1.14.13.122 protochlorophyllide a + O2 + NADPH low activity Arabidopsis thaliana 7-hydroxychlorophyllide a + H2O + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.122 More the enzyme is part of a separate translocon complex in chlorplasts Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.14.13.122 CAO
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.122 23
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.122 NADPH using a ferredoxin-NADPH reduction system in activity assays Arabidopsis thaliana