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Literature summary extracted from

  • Ruppert, M.; Woll, J.; Giritch, A.; Genady, E.; Ma, X.; Stöckigt, J.
    Functional expression of an ajmaline pathway-specific esterase from Rauvolfia in a novel plant-virus expression system (2005), Planta, 222, 888-898.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.1.1.80 the expression in Escherichia coli failed although a wide range of conditions were tested. With a virus-based plant expression system, it was possible to express the enzyme functionally in leaves of Nicotiana benthamiana Domin. An extraordinarily high enzyme activity is detected in the Nicotiana tissue, which exceeds that in Rauvolfia cell suspension cultures about 20fold Rauvolfia verticillata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.1.80 0.12
-
 17-O-acetylajmaline
-
 Rauvolfia verticillata
3.1.1.80 0.7
-
 17-O-acetylnorajmaline
-
 Rauvolfia verticillata

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.1.80 40000
-
 gel filtration Rauvolfia verticillata

Organism

EC Number Organism UniProt Comment Textmining
3.1.1.80 Rauvolfia serpentina Q3MKY2 L. Benth. ex Kurz
-
3.1.1.80 Rauvolfia verticillata
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.1.80
-
 Rauvolfia verticillata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.1.80 cell suspension culture
-
 Rauvolfia verticillata
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.1.80 2.484
-
-
 Rauvolfia verticillata

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.1.80 17-O-acetylajmaline + H2O
-
 Rauvolfia verticillata ajmaline + acetate
-
 ?
3.1.1.80 17-O-acetylnorajmaline + H2O 70.2% of the activity with 17-O-acetylajmaline Rauvolfia verticillata norajmaline + acetate
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.1.80 monomer 1 * 40000, SDS-PAGE Rauvolfia verticillata

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.1.1.80 53
-
-
 Rauvolfia verticillata

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.1.1.80 8
-
-
 Rauvolfia verticillata

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.1.1.80 6.5 9.8 pH 6.5 and pH 9.8: about 50% of maximal activity Rauvolfia verticillata

pI Value

EC Number Organism Comment pI Value Maximum pI Value
3.1.1.80 Rauvolfia verticillata calculated from sequence
-
 7.65