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Literature summary extracted from

  • Makarevitch, I.; Somers, D.A.
    Purification and characterization of topoisomerase IIA from Arabidopsis thaliana (2005), Plant Sci., 168, 1023-1033.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 etoposide inhibits the enzyme at the DNA relaxation step Arabidopsis thaliana
5.6.2.2 m-AMSA inhibits the enzyme at the DNA relaxation step Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
5.6.2.2 additional information
-
 additional information kinetics Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 nucleus
-
 Arabidopsis thaliana 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 Co2+ can substitute Mg2+ in a concentration range of 5-10 mM, less effective Arabidopsis thaliana
5.6.2.2 Cu2+ can substitute Mg2+ in a concentration range of 5-10 mM, less effective Arabidopsis thaliana
5.6.2.2 KCl required for DNA relaxation, optimal at 100-150 mM Arabidopsis thaliana
5.6.2.2 Mg2+ dependent on, preferred divalent cation, most active at 5.0-7.5 mM Arabidopsis thaliana
5.6.2.2 Mn2+ can substitute Mg2+ in a concentration range of 5-10 mM, less effective Arabidopsis thaliana
5.6.2.2 additional information no activity with Ca2+ Arabidopsis thaliana
5.6.2.2 NaCl required for DNA relaxation, optimal at 100-150 mM Arabidopsis thaliana

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Arabidopsis thaliana
-
 ecotype Columbia, nuclear encoded enzyme
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.6.2.2 Topo IIA 1100fold from seeds in a 5-step process, to homogeneity Arabidopsis thaliana

Reaction

EC Number Reaction Comment Organism Reaction ID
5.6.2.2 ATP-dependent breakage, passage and rejoining of double-stranded DNA relaxation mechanism Arabidopsis thaliana
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 seed
-
 Arabidopsis thaliana
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
5.6.2.2 additional information
-
 relaxation activity of the purified Topo IIA Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 additional information substrate specificity Arabidopsis thaliana ?
-
 ?
5.6.2.2 supercoiled dsDNA relaxation of supercoiled dsDNA, reaction mechanism Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 Topo IIA
-
 Arabidopsis thaliana
5.6.2.2 topoisomerase IIA
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.2 25
-
 assay at Arabidopsis thaliana

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
5.6.2.2 additional information
-
 purified Topo IIA is temperature-labile Arabidopsis thaliana

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.2 8
-
 assay at Arabidopsis thaliana

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
5.6.2.2 additional information
-
 purified Topo IIA is pH-labile Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP
-
 Arabidopsis thaliana