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Literature summary extracted from

  • Marri, L.; Trost, P.; Pupillo, P.; Sparla, F.
    Reconstitution and properties of the recombinant glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase supramolecular complex of Arabidopsis (2005), Plant Physiol., 139, 1433-1443.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.19 dithiothreitol reduced form, required for full activity Arabidopsis thaliana
2.7.1.19 thioredoxin reduced form, regulates activity via reversible reduction Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.12 into the pET28a vector for expression in Escherichia coli BL21DE3 cells, amplificatied in Escherichia coli HB101 cells Arabidopsis thaliana
2.7.1.19 expressed in Escherichia coli Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.2.1.12 dithiothreitol DTT Arabidopsis thaliana
1.2.1.12 H2O2
-
 Arabidopsis thaliana
2.7.1.19 additional information oxidizing agents inhibit enzyme activity Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.19 chloroplast
-
 Arabidopsis thaliana 9507
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.2.1.12 36000
-
 monomer, determined by SDS-PAGE Arabidopsis thaliana
1.2.1.12 120000
-
 tetramer, determined by gel filtration Arabidopsis thaliana
2.7.1.19 97000
-
 isolated enzyme, reduced form, gel filtration Arabidopsis thaliana
2.7.1.19 110000
-
 isolated enzyme, oxidized form, gel filtration Arabidopsis thaliana
2.7.1.19 640000
-
 glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex, gel filtration Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH + H+ Arabidopsis thaliana
-
 D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
2.7.1.19 ATP + D-ribulose 5-phosphate Arabidopsis thaliana part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms ADP + D-ribulose 1,5-bisphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.12 Arabidopsis thaliana
-
-
-
2.7.1.19 Arabidopsis thaliana
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.12 purification protocol comprises two anion-exchange chromatographic steps and removing of the His tag Arabidopsis thaliana

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.12 3-phospho-D-glyceroyl phosphate + NADH + H+
-
 Arabidopsis thaliana D-glyceraldehyde 3-phosphate + phosphate + NAD+
-
 ?
2.7.1.19 ATP + D-ribulose 5-phosphate
-
 Arabidopsis thaliana ADP + D-ribulose 1,5-bisphosphate
-
 ?
2.7.1.19 ATP + D-ribulose 5-phosphate part of the Calvin cycle (reductive pentose phosphate pathway), involved in CO2 fixation in photosynthetic organisms Arabidopsis thaliana ADP + D-ribulose 1,5-bisphosphate
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.12 homotetramer
-
 Arabidopsis thaliana
2.7.1.19 dimer isolated enzyme Arabidopsis thaliana
2.7.1.19 multimer glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase complex, native form of enzyme with a molecular weight of 640000 Da Arabidopsis thaliana

Synonyms

EC Number Synonyms Comment Organism
1.2.1.12 GapA A4 isoform of GAPDH Arabidopsis thaliana
1.2.1.12 GAPDH
-
 Arabidopsis thaliana
1.2.1.12 glyceraldehyde-3-phosphate dehydrogenase
-
 Arabidopsis thaliana
2.7.1.19 glyceraldehyde-3-phosphate dehydrogenase/CP12/phosphoribulokinase glyceraldehyde-3-phosphate dehydrogenase and phosphoribulokinase form a supramolecular complex with the regulatory peptide CP12, both enzyme activities strongly dependent on complex formation Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.12 NADH
-
 Arabidopsis thaliana