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Literature summary extracted from

  • Coursol, S.; Le Stunff, H.; Lynch, D.V.; Gilroy, S.; Assmann, S.M.; Spiegel, S.
    Arabidopsis sphingosine kinase and the effects of phytosphingosine-1-phosphate on stomatal aperture (2005), Plant Physiol., 137, 724-737.
    View publication on PubMedView publication on EuropePMC

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.91 bovine serum albumin the enzyme activity is higher with substrate solubilized by bovine serum albumin compared to Triton X-100 Arabidopsis thaliana
2.7.1.91 additional information the enzyme is stimulated by the phytohormone abscisic acid in guard cells and mesophyll cell protoplasts Arabidopsis thaliana

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.91 phylogenetic analysis Arabidopsis thaliana

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.91 DL-threo-dihydrosphingosine slight inhibition Arabidopsis thaliana
2.7.1.91 N,N-dimethylsphingosine strong inhibition Arabidopsis thaliana
2.7.1.91 Triton X-100 the enzyme activity is higher with substrate solubilized by bovine serum albumin compared to Triton X-100 Arabidopsis thaliana

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.91 additional information
-
 additional information kinetics Arabidopsis thaliana

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.91 cytosol the cytosolic enzyme is 20fold less active than the membrane-associated enzyme Arabidopsis thaliana 5829
-
2.7.1.91 membrane 75% of total activity in leaves, associated with, the cytosolic enzyme is 20fold less active than the membrane-associated enzyme Arabidopsis thaliana 16020
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.91 KCl activates Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.91 ATP + phytosphingosine Arabidopsis thaliana phytosphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins ADP + phytosphingosine 1-phosphate
-
 ir
2.7.1.91 ATP + sphingosine Arabidopsis thaliana sphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins ADP + sphingosine 1-phosphate
-
 ir

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.91 Arabidopsis thaliana
-
 several isozymes
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.91 guard cell
-
 Arabidopsis thaliana
-
2.7.1.91 leaf
-
 Arabidopsis thaliana
-
2.7.1.91 mesophyll
-
 Arabidopsis thaliana
-
2.7.1.91 stoma
-
 Arabidopsis thaliana
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.91 ATP + 4,8-sphingadienine high activity Arabidopsis thaliana ADP + 4,8-sphingadienine 1-phosphate
-
 ir
2.7.1.91 ATP + DELTA4-unsaturated long chain sphingosine high activity Arabidopsis thaliana ADP + DELTA4-unsaturated long chain sphingosine 1-phosphate
-
 ir
2.7.1.91 ATP + phytosphingosine phytosphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins Arabidopsis thaliana ADP + phytosphingosine 1-phosphate
-
 ir
2.7.1.91 ATP + phytosphingosine i.e. 4-hydroxysphinganine, moderate or low activity Arabidopsis thaliana ADP + phytosphingosine 1-phosphate
-
 ir
2.7.1.91 ATP + sphinganine i.e. dihydrosphingosine, moderate or low activity Arabidopsis thaliana ADP + sphinganine 1-phosphate
-
 ir
2.7.1.91 ATP + sphingosine
-
 Arabidopsis thaliana ADP + sphingosine 1-phosphate
-
 ir
2.7.1.91 ATP + sphingosine sphingosine 1-phosphate is capable of regulating the turgor in guard cells/stomatal aperture via G-proteins Arabidopsis thaliana ADP + sphingosine 1-phosphate
-
 ir
2.7.1.91 additional information substrate specificity of cytosolic and membrane enzymes, 4-hydroxy-8-sphingenine is a poor substrate Arabidopsis thaliana ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.1.91 sphinganine kinase
-
 Arabidopsis thaliana
2.7.1.91 SPHK
-
 Arabidopsis thaliana

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.91 37
-
 assay at Arabidopsis thaliana

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.91 ATP
-
 Arabidopsis thaliana