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Literature summary extracted from

  • Huang, F.; Haydock, S.F.; Mironenko, T.; Spiteller, D.; Li, Y.; Spencer, J.B.
    The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterization of an aminotransferase involved in the formation of 2-deoxystreptamine (2005), Org. Biomol. Chem., 3, 1410-1418.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.50
-
 Streptomyces fradiae
2.6.1.101 gene neo-6, DNA and amino acid sequence determination and analysis, overexpression as His6-tagged enzyme in Esccherichia coli Streptomyces fradiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.50 45000
-
 x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE Streptomyces fradiae
2.6.1.50 46951
-
 x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE Streptomyces fradiae
2.6.1.101 45000
-
 x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry Streptomyces fradiae
2.6.1.101 46801
-
 x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry Streptomyces fradiae
2.6.1.101 46951
-
 x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry Streptomyces fradiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae
-
 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose Streptomyces fradiae NCIMB 8233
-
 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
2.6.1.100 additional information Streptomyces fradiae purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 ?
-
 ?
2.6.1.100 additional information Streptomyces fradiae NCIMB 8233 purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 ?
-
 ?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose Streptomyces fradiae
-
 2-oxoglutaramate + 2-deoxystreptamine
-
 ?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose Streptomyces fradiae NCIMB 8233
-
 2-oxoglutaramate + 2-deoxystreptamine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.50 Streptomyces fradiae Q53U20 overexpression in Escherichia coli
-
2.6.1.50 Streptomyces fradiae NCIMB 8233 Q53U20 overexpression in Escherichia coli
-
2.6.1.100 Streptomyces fradiae
-
-
-
2.6.1.100 Streptomyces fradiae
-
 gene tbmBr
-
2.6.1.100 Streptomyces fradiae NCIMB 8233
-
-
-
2.6.1.100 Streptomyces fradiae NCIMB 8233
-
 gene tbmBr
-
2.6.1.101 Streptomyces fradiae
-
-
-
2.6.1.101 Streptomyces fradiae Q53U20 gene neo-6 encoded in the neo gene cluster
-
2.6.1.101 Streptomyces fradiae NCIMB 8233
-
-
-
2.6.1.101 Streptomyces fradiae NCIMB 8233 Q53U20 gene neo-6 encoded in the neo gene cluster
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.50 2-deoxy-scyllo-inosose + L-glutamine
-
 Streptomyces fradiae 2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
 ?
2.6.1.50 2-deoxy-scyllo-inosose + L-glutamine
-
 Streptomyces fradiae NCIMB 8233 2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
 ?
2.6.1.50 2-deoxystreptamine + pyruvate
-
 Streptomyces fradiae 2-keto-inosose + L-alanine
-
 r
2.6.1.50 2-deoxystreptamine + pyruvate
-
 Streptomyces fradiae NCIMB 8233 2-keto-inosose + L-alanine
-
 r
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose
-
 Streptomyces fradiae 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
2.6.1.100 L-glutamine + 2-deoxy-scyllo-inosose
-
 Streptomyces fradiae NCIMB 8233 2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
 ?
2.6.1.100 additional information purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 Streptomyces fradiae ?
-
 ?
2.6.1.100 additional information purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101 Streptomyces fradiae NCIMB 8233 ?
-
 ?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
 Streptomyces fradiae 2-oxoglutaramate + 2-deoxystreptamine
-
 ?
2.6.1.101 L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
 Streptomyces fradiae NCIMB 8233 2-oxoglutaramate + 2-deoxystreptamine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.6.1.50 ? x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE Streptomyces fradiae
2.6.1.101 ? x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry Streptomyces fradiae

Synonyms

EC Number Synonyms Comment Organism
2.6.1.50 neo-6
-
 Streptomyces fradiae
2.6.1.100 btrR
-
 Streptomyces fradiae
2.6.1.100 BtrS
-
 Streptomyces fradiae
2.6.1.100 kanB
-
 Streptomyces fradiae
2.6.1.100 neo-6
-
 Streptomyces fradiae
2.6.1.100 neoB
-
 Streptomyces fradiae
2.6.1.101 btrR
-
 Streptomyces fradiae
2.6.1.101 BtrS
-
 Streptomyces fradiae
2.6.1.101 L-glutamine: 2-deoxy-scyllo-inosamine aminotransferase
-
 Streptomyces fradiae
2.6.1.101 L-glutamine:DOI aminotransferase
-
 Streptomyces fradiae
2.6.1.101 neo-6
-
 Streptomyces fradiae

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.6.1.100 37
-
 assay at Streptomyces fradiae
2.6.1.101 37
-
 assay at Streptomyces fradiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.100 7.5
-
 assay at Streptomyces fradiae
2.6.1.101 7.5
-
 assay at Streptomyces fradiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.100 pyridoxal 5'-phosphate the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity Streptomyces fradiae
2.6.1.101 pyridoxal 5'-phosphate the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity Streptomyces fradiae

General Information

EC Number General Information Comment Organism
2.6.1.100 metabolism Neo-6 is the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis. Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101, the aglycon in neomycins, overview Streptomyces fradiae
2.6.1.100 additional information gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview Streptomyces fradiae
2.6.1.100 physiological function the enzyme produces 2-deoxystreptamine (DOS), EC 2.6.1.101, which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis Streptomyces fradiae
2.6.1.101 evolution BtrR and StsC are the two homologues of Neo-6 in butirosin and streptamycin biosynthetic pathways Streptomyces fradiae
2.6.1.101 metabolism purified Neo-6 also catalyses the amino transfer from L-glutamine to 2-deoxyscyllo-inosose to produce 2-deoxyscyllo-inosamine when coupled with BtrC in the presence of G6P, NAD+, Co2+ and L-glutamine, EC 2.6.1.100 Streptomyces fradiae
2.6.1.101 additional information gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview Streptomyces fradiae
2.6.1.101 physiological function the enzyme produces 2-deoxystreptamine (DOS), which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis, function of Neo-6 as the L-glutamine:DOI aminotransferase Streptomyces fradiae