BRENDA - Enzyme Database

The neomycin biosynthetic gene cluster of Streptomyces fradiae NCIMB 8233: characterization of an aminotransferase involved in the formation of 2-deoxystreptamine

Huang, F.; Haydock, S.F.; Mironenko, T.; Spiteller, D.; Li, Y.; Spencer, J.B.; Org. Biomol. Chem. 3, 1410-1418 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.6.1.50
-
Streptomyces fradiae
2.6.1.101
gene neo-6, DNA and amino acid sequence determination and analysis, overexpression as His6-tagged enzyme in Esccherichia coli
Streptomyces fradiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.50
45000
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
2.6.1.50
46951
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
2.6.1.101
45000
-
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
2.6.1.101
46801
-
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
2.6.1.101
46951
-
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
Streptomyces fradiae
-
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
?
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
Streptomyces fradiae NCIMB 8233
-
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
?
2.6.1.100
additional information
Streptomyces fradiae
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
?
-
-
-
2.6.1.100
additional information
Streptomyces fradiae NCIMB 8233
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
?
-
-
-
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
Streptomyces fradiae
-
2-oxoglutaramate + 2-deoxystreptamine
-
-
?
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
Streptomyces fradiae NCIMB 8233
-
2-oxoglutaramate + 2-deoxystreptamine
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.6.1.50
Streptomyces fradiae
Q53U20
overexpression in Escherichia coli
-
2.6.1.50
Streptomyces fradiae NCIMB 8233
Q53U20
overexpression in Escherichia coli
-
2.6.1.100
Streptomyces fradiae
-
; gene tbmBr
-
2.6.1.100
Streptomyces fradiae NCIMB 8233
-
; gene tbmBr
-
2.6.1.101
Streptomyces fradiae
Q53U20
gene neo-6 encoded in the neo gene cluster
-
2.6.1.101
Streptomyces fradiae
-
-
-
2.6.1.101
Streptomyces fradiae NCIMB 8233
Q53U20
gene neo-6 encoded in the neo gene cluster
-
2.6.1.101
Streptomyces fradiae NCIMB 8233
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.50
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2.6.1.50
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae NCIMB 8233
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2.6.1.50
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae
2-keto-inosose + L-alanine
-
-
-
r
2.6.1.50
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae NCIMB 8233
2-keto-inosose + L-alanine
-
-
-
r
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
-
663004
Streptomyces fradiae
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
-
?
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
-
663004
Streptomyces fradiae NCIMB 8233
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
-
?
2.6.1.100
additional information
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
663004
Streptomyces fradiae
?
-
-
-
-
2.6.1.100
additional information
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
663004
Streptomyces fradiae NCIMB 8233
?
-
-
-
-
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
663004
Streptomyces fradiae
2-oxoglutaramate + 2-deoxystreptamine
-
-
-
?
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
663004
Streptomyces fradiae NCIMB 8233
2-oxoglutaramate + 2-deoxystreptamine
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
2.6.1.50
?
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
2.6.1.101
?
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.100
37
-
assay at
Streptomyces fradiae
2.6.1.101
37
-
assay at
Streptomyces fradiae
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.100
7.5
-
assay at
Streptomyces fradiae
2.6.1.101
7.5
-
assay at
Streptomyces fradiae
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.100
pyridoxal 5'-phosphate
the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity
Streptomyces fradiae
2.6.1.101
pyridoxal 5'-phosphate
the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity
Streptomyces fradiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.6.1.50
-
Streptomyces fradiae
2.6.1.101
gene neo-6, DNA and amino acid sequence determination and analysis, overexpression as His6-tagged enzyme in Esccherichia coli
Streptomyces fradiae
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
2.6.1.100
pyridoxal 5'-phosphate
the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity
Streptomyces fradiae
2.6.1.101
pyridoxal 5'-phosphate
the BtrR-bound pyridoxal 5'-phosphate is the essential cofactor for the amino-transfer activity
Streptomyces fradiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.6.1.50
45000
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
2.6.1.50
46951
-
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
2.6.1.101
45000
-
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
2.6.1.101
46801
-
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
2.6.1.101
46951
-
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
Streptomyces fradiae
-
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
?
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
Streptomyces fradiae NCIMB 8233
-
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
?
2.6.1.100
additional information
Streptomyces fradiae
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
?
-
-
-
2.6.1.100
additional information
Streptomyces fradiae NCIMB 8233
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
?
-
-
-
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
Streptomyces fradiae
-
2-oxoglutaramate + 2-deoxystreptamine
-
-
?
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
Streptomyces fradiae NCIMB 8233
-
2-oxoglutaramate + 2-deoxystreptamine
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.6.1.50
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2.6.1.50
2-deoxy-scyllo-inosose + L-glutamine
-
663004
Streptomyces fradiae NCIMB 8233
2-deoxy-scyllo-inosamine + 2-ketoglutaramate
-
-
-
?
2.6.1.50
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae
2-keto-inosose + L-alanine
-
-
-
r
2.6.1.50
2-deoxystreptamine + pyruvate
-
663004
Streptomyces fradiae NCIMB 8233
2-keto-inosose + L-alanine
-
-
-
r
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
-
663004
Streptomyces fradiae
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
-
?
2.6.1.100
L-glutamine + 2-deoxy-scyllo-inosose
-
663004
Streptomyces fradiae NCIMB 8233
2-oxoglutaramate + 2-deoxy-scyllo-inosamine
-
-
-
?
2.6.1.100
additional information
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
663004
Streptomyces fradiae
?
-
-
-
-
2.6.1.100
additional information
purified Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101
663004
Streptomyces fradiae NCIMB 8233
?
-
-
-
-
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
663004
Streptomyces fradiae
2-oxoglutaramate + 2-deoxystreptamine
-
-
-
?
2.6.1.101
L-glutamine + 3-amino-2,3-dideoxy-scyllo-inosose
-
663004
Streptomyces fradiae NCIMB 8233
2-oxoglutaramate + 2-deoxystreptamine
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
2.6.1.50
?
x * 46951, His6-tagged protein, claculated, x * 45000, His6-tagged protein, SDS-PAGE
Streptomyces fradiae
2.6.1.101
?
x * 46951, sequence calculation, x * 45000, recombinant His6-tagged enzyme, SDS-PAGE, x * 46801, recombinant His6-tagged enzyme, mass spectrometry
Streptomyces fradiae
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
2.6.1.100
37
-
assay at
Streptomyces fradiae
2.6.1.101
37
-
assay at
Streptomyces fradiae
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.6.1.100
7.5
-
assay at
Streptomyces fradiae
2.6.1.101
7.5
-
assay at
Streptomyces fradiae
General Information
EC Number
General Information
Commentary
Organism
2.6.1.100
metabolism
Neo-6 is the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis. Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101, the aglycon in neomycins, overview
Streptomyces fradiae
2.6.1.100
additional information
gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview
Streptomyces fradiae
2.6.1.100
physiological function
the enzyme produces 2-deoxystreptamine (DOS), EC 2.6.1.101, which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis
Streptomyces fradiae
2.6.1.101
evolution
BtrR and StsC are the two homologues of Neo-6 in butirosin and streptamycin biosynthetic pathways
Streptomyces fradiae
2.6.1.101
metabolism
purified Neo-6 also catalyses the amino transfer from L-glutamine to 2-deoxyscyllo-inosose to produce 2-deoxyscyllo-inosamine when coupled with BtrC in the presence of G6P, NAD+, Co2+ and L-glutamine, EC 2.6.1.100
Streptomyces fradiae
2.6.1.101
additional information
gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview
Streptomyces fradiae
2.6.1.101
physiological function
the enzyme produces 2-deoxystreptamine (DOS), which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis, function of Neo-6 as the L-glutamine:DOI aminotransferase
Streptomyces fradiae
General Information (protein specific)
EC Number
General Information
Commentary
Organism
2.6.1.100
metabolism
Neo-6 is the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis. Neo-6 also catalyses the amino transfer from L-glutamine to 3-amino-2,3-dideoxy-scyllo-inosose to produce 2-deoxystrepamine, EC 2.6.1.101, the aglycon in neomycins, overview
Streptomyces fradiae
2.6.1.100
additional information
gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview
Streptomyces fradiae
2.6.1.100
physiological function
the enzyme produces 2-deoxystreptamine (DOS), EC 2.6.1.101, which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis
Streptomyces fradiae
2.6.1.101
evolution
BtrR and StsC are the two homologues of Neo-6 in butirosin and streptamycin biosynthetic pathways
Streptomyces fradiae
2.6.1.101
metabolism
purified Neo-6 also catalyses the amino transfer from L-glutamine to 2-deoxyscyllo-inosose to produce 2-deoxyscyllo-inosamine when coupled with BtrC in the presence of G6P, NAD+, Co2+ and L-glutamine, EC 2.6.1.100
Streptomyces fradiae
2.6.1.101
additional information
gene neo6 of the neo gene cluster and btrR from the btr gene cluster are equivalent, genetic organization, overview
Streptomyces fradiae
2.6.1.101
physiological function
the enzyme produces 2-deoxystreptamine (DOS), which is the aglycone in neomycins, overview. Neo-6 is also the enzyme catalysing the transamination of 2-deoxy-scyllo-inosose, DOI, using L-glutamine as an amino donor to produce 2-deoxyscyllo-inosamine as required for 2-deoxystreptamine biosynthesis, function of Neo-6 as the L-glutamine:DOI aminotransferase
Streptomyces fradiae