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Literature summary extracted from

  • Breen, R.S.; Campopiano, D.J.; Webster, S.; Brunton, M.; Watt, R.; Baxter, R.L.
    The mechanism of 7,8-diaminopelargonate synthase; the role of S-adenosylmethionine as the amino donor (2003), Org. Biomol. Chem., 1, 3498-3499.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.62 cloning from strain JM101, overexpression in strain BL21(DE3) Escherichia coli

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.62 amiclenomycin
-
 Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.62 additional information
-
 additional information kinetic analysis Escherichia coli

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate Escherichia coli enzyme is obligate catalyzing the second step in the biosynthesis of vitamin H, i.e. biotin S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.62 Escherichia coli
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.62 recombinant enzyme from strain BL21(DE3) Escherichia coli

Reaction

EC Number Reaction Comment Organism Reaction ID
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate = S-adenosyl-4-(methylsulfanyl)-2-oxobutanoate + 7,8-diaminononanoate ping pong kinetics, reaction mechanism, via a enzyme-pyridoxamine phosphate, a quinonoid intermediate Escherichia coli
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate enzyme is obligate catalyzing the second step in the biosynthesis of vitamin H, i.e. biotin Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 r
2.6.1.62 S-adenosyl-L-methionine + 8-amino-7-oxononanoate stereospecific amination Escherichia coli S-adenosyl-4-methylthio-2-oxobutanoate + (7S,8R)-diaminononanoate
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.62 7,8-diaminopelargonate synthase
-
 Escherichia coli
2.6.1.62 DAPA synthase
-
 Escherichia coli

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.6.1.62 7.5
-
 assay at Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.62 pyridoxal 5'-phosphate dependent on Escherichia coli