EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.7.7 | D473G | wild-type variant Pfu-Pol(exo-) is is 60fold less accurate than Pfu-Pol(exo+) | Pyrococcus furiosus |
2.7.7.7 | T471A | less accurate, by factors of 1.6 than the wild-type variant Pfu-Pol(exo-) | Pyrococcus furiosus |
2.7.7.7 | T471G | less accurate, by factors of 1.2 than the wild-type variant Pfu-Pol(exo-) | Pyrococcus furiosus |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.7.7 | Pyrococcus furiosus | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.7.7 | - |
Pyrococcus furiosus |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.7.7 | deoxynucleoside triphosphate + DNAn | wild-type Pfu-Pol makes about one mistake for every 1000000 bases incorporated, wild-type variant Pfu-Pol(exo-)(D473F)is 60fold less accurate | Pyrococcus furiosus | diphosphate + DNAn+1 | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.7.7 | Pfu-POl | - |
Pyrococcus furiosus |