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Literature summary extracted from

  • Zhu, H.; Klemic, J.F.; Chang, S.; Bertone, P.; Casamayor, A.; Klemic, K.G.; Smith, D.; Gerstein, M.; Reed, M.A.; Snyder, M.
    Analysis of yeast protein kinases using protein chips (2000), Nat. Genet., 26, 283-289.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.11.22 cyclin dependent on Saccharomyces cerevisiae

Application

EC Number Application Comment Organism
2.7.10.2 analysis development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview Saccharomyces cerevisiae
2.7.11.22 analysis development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview Saccharomyces cerevisiae
2.7.11.24 analysis development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview Saccharomyces cerevisiae
2.7.11.25 analysis development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview Saccharomyces cerevisiae
2.7.12.1 analysis development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview Saccharomyces cerevisiae
2.7.12.2 analysis development of a protein chip consisting of a silicone elastomer microwell array with recombinant enzyme covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker for large scale activity assay, overview Saccharomyces cerevisiae

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.10.2 phylogenetic tree of kinases derived from the kinase core sequence, overview, expression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences Saccharomyces cerevisiae
2.7.11.22 phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences Saccharomyces cerevisiae
2.7.11.24 phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences Saccharomyces cerevisiae
2.7.11.25 phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences Saccharomyces cerevisiae
2.7.12.1 phylogenetic tree of kinases derived from the kinase core sequence, overview, overexpression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences Saccharomyces cerevisiae
2.7.12.2 phylogenetic tree of kinases derived from the kinase core sequence, overview, expression as GST-fusion protein under control of the galactose-inducible GAL1 promotor in Escherichia coli, determination of 5'-end sequences Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.10.2 Mg2+
-
 Saccharomyces cerevisiae
2.7.11.22 Mg2+
-
 Saccharomyces cerevisiae
2.7.11.24 Mg2+
-
 Saccharomyces cerevisiae
2.7.11.25 Mg2+
-
 Saccharomyces cerevisiae
2.7.12.1 Mg2+
-
 Saccharomyces cerevisiae
2.7.12.2 Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.10.2 ATP + a protein Saccharomyces cerevisiae
-
 ADP + a phosphoprotein
-
 ?
2.7.11.22 ATP + a protein Saccharomyces cerevisiae
-
 ADP + a phosphoprotein
-
 ?
2.7.11.24 ATP + a protein Saccharomyces cerevisiae
-
 ADP + a phosphoprotein
-
 ?
2.7.11.24 additional information Saccharomyces cerevisiae Hog1 is related to osmotic stress ?
-
 ?
2.7.11.25 ATP + a protein Saccharomyces cerevisiae
-
 ADP + a phosphoprotein
-
 ?
2.7.12.1 ATP + a protein Saccharomyces cerevisiae
-
 ADP + a phosphoprotein
-
 ?
2.7.12.2 ATP + a protein Saccharomyces cerevisiae
-
 ADP + a phosphoprotein
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.10.2 Saccharomyces cerevisiae
-
-
-
2.7.11.22 Saccharomyces cerevisiae
-
-
-
2.7.11.24 Saccharomyces cerevisiae
-
-
-
2.7.11.25 Saccharomyces cerevisiae
-
-
-
2.7.12.1 Saccharomyces cerevisiae
-
-
-
2.7.12.2 Saccharomyces cerevisiae
-
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
2.7.10.2 phosphoprotein the enzyme performs autophosphorylation Saccharomyces cerevisiae
2.7.11.22 phosphoprotein the enzyme performs autophosphorylation Saccharomyces cerevisiae
2.7.11.24 phosphoprotein Hog1 performs autophosphorylation, while Fus3 does not Saccharomyces cerevisiae
2.7.11.25 phosphoprotein the enzyme performs autophosphorylation Saccharomyces cerevisiae
2.7.12.1 phosphoprotein the enzyme performs autophosphorylation Saccharomyces cerevisiae
2.7.12.2 phosphoprotein the enzyme performs autophosphorylation Saccharomyces cerevisiae

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.10.2 additional information
-
 large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview Saccharomyces cerevisiae
2.7.11.22 additional information
-
 large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview Saccharomyces cerevisiae
2.7.11.24 additional information
-
 large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview Saccharomyces cerevisiae
2.7.11.25 additional information
-
 large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview Saccharomyces cerevisiae
2.7.12.1 additional information
-
 large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview Saccharomyces cerevisiae
2.7.12.2 additional information
-
 large scale activity assay with recombinant enzyme in a protein chip consisting of a microwell array with protein covalently attached to the wells via a 3-glycidoxypropyltrimethoxysilane crosslinker, overview Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.10.2 ATP + a protein
-
 Saccharomyces cerevisiae ADP + a phosphoprotein
-
 ?
2.7.10.2 ATP + poly-(Tyr-Glu) tyrosine kinase substrate, phosphoryated by Swe1p, Mps1p, Ime2p, and Hrr25p, but not by Ste7p, Rad53p, or Mck1p Saccharomyces cerevisiae ADP + phospho-poly-(Tyr-Glu)
-
 ?
2.7.11.22 ATP + a protein
-
 Saccharomyces cerevisiae ADP + a phosphoprotein
-
 ?
2.7.11.22 ATP + Swi6 substrate of cdc28p Saccharomyces cerevisiae ADP + phosphorylated Swi6
-
 ?
2.7.11.24 ATP + a protein
-
 Saccharomyces cerevisiae ADP + a phosphoprotein
-
 ?
2.7.11.24 ATP + Axl2 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Axl2
-
 ?
2.7.11.24 ATP + casein substrate of Hog1 Saccharomyces cerevisiae ADP + phosphocasein
-
 ?
2.7.11.24 ATP + cdc42 substrate of Gic2 Saccharomyces cerevisiae ADP + phosphorylated cdc42
-
 ?
2.7.11.24 ATP + Gic2 substrate of Fus3, and of Hog1 Saccharomyces cerevisiae ADP + phosphorylated Gic2
-
 ?
2.7.11.24 ATP + histone H1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-histone H1
-
 ?
2.7.11.24 ATP + Hog1D substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Hog1D
-
 ?
2.7.11.24 ATP + Hsl1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Hsl1
-
 ?
2.7.11.24 ATP + MBP substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-MBP
-
 ?
2.7.11.24 ATP + Mek1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Mek1
-
 ?
2.7.11.24 ATP + protein tyrosine kinase 2 substrate of Hog1 Saccharomyces cerevisiae ADP + phosphorylated protein tyrosine kinase 2
-
 ?
2.7.11.24 ATP + RAD9 high activity with Fus3, low activity with Hog1 Saccharomyces cerevisiae ADP + phospho-RAD9
-
 ?
2.7.11.24 ATP + RAD9p substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-RAD9p
-
 ?
2.7.11.24 ATP + Red1 preferred substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Red1
-
 ?
2.7.11.24 ATP + Swe1 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Swe1
-
 ?
2.7.11.24 ATP + Swi6 substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Swi6
-
 ?
2.7.11.24 ATP + Tub4p substrate of Hog1 Saccharomyces cerevisiae ADP + phospho-Tub4
-
 ?
2.7.11.24 additional information Hog1 is related to osmotic stress Saccharomyces cerevisiae ?
-
 ?
2.7.11.24 additional information the enzyme performs autophosphorylation Saccharomyces cerevisiae ?
-
 ?
2.7.11.25 ATP + a protein
-
 Saccharomyces cerevisiae ADP + a phosphoprotein
-
 ?
2.7.11.25 ATP + Mek1
-
 Saccharomyces cerevisiae ADP + phospho-Mek1
-
 ?
2.7.12.1 ATP + a protein
-
 Saccharomyces cerevisiae ADP + a phosphoprotein
-
 ?
2.7.12.1 ATP + poly-(Tyr-Glu) tyrosine kinase substrate Saccharomyces cerevisiae ADP + phospho-poly-(Tyr-Glu)
-
 ?
2.7.12.2 ATP + a protein
-
 Saccharomyces cerevisiae ADP + a phosphoprotein
-
 ?
2.7.12.2 ATP + poly-(Tyr-Glu) tyrosine kinase substrate Saccharomyces cerevisiae ADP + phospho-poly-(Tyr-Glu)
-
 ?
2.7.12.2 ATP + Red1 substrate of Mek1 Saccharomyces cerevisiae ADP + phospho-Red1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.10.2 Hrr25p
-
 Saccharomyces cerevisiae
2.7.10.2 Ime2p
-
 Saccharomyces cerevisiae
2.7.10.2 Mps1p
-
 Saccharomyces cerevisiae
2.7.10.2 Swe1p
-
 Saccharomyces cerevisiae
2.7.11.22 cdc28p
-
 Saccharomyces cerevisiae
2.7.11.22 cdc42p
-
 Saccharomyces cerevisiae
2.7.11.24 Fus3
-
 Saccharomyces cerevisiae
2.7.11.24 Hog1
-
 Saccharomyces cerevisiae
2.7.11.24 Mek1p
-
 Saccharomyces cerevisiae
2.7.11.25 Mek kinase
-
 Saccharomyces cerevisiae
2.7.12.1 protein tyrosine kinase 2
-
 Saccharomyces cerevisiae
2.7.12.1 PTK2
-
 Saccharomyces cerevisiae
2.7.12.2 MEK1
-
 Saccharomyces cerevisiae
2.7.12.2 PTK2
-
 Saccharomyces cerevisiae
2.7.12.2 TUB4
-
 Saccharomyces cerevisiae

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.10.2 ATP
-
 Saccharomyces cerevisiae
2.7.11.22 ATP
-
 Saccharomyces cerevisiae
2.7.11.24 ATP
-
 Saccharomyces cerevisiae
2.7.11.25 ATP
-
 Saccharomyces cerevisiae
2.7.12.1 ATP
-
 Saccharomyces cerevisiae
2.7.12.2 ATP
-
 Saccharomyces cerevisiae