Literature summary extracted from

Stonehouse, M.J.; Cota-Gomez, A.; Parker, S.K.; Martin, W.E.; Hankin, J.A.; Murphy, R.C.; Chen, W.; Lim, K.B.; Hackett, M.; Vasil, A.I.; Vasil, M.L.
A novel class of microbial phosphocholine-specific phospholipases C (2002), Mol. Microbiol., 46, 661-676.

Inhibitors

EC Number	Inhibitors	Comment	Organism	Structure
3.1.4.3	additional information	no inhibition of enzymatic activity by Ca2+, inhibits haemolytic activity of the enzyme	Pseudomonas aeruginosa

KM Value [mM]

EC Number	KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
3.1.4.3	14.1	-	p-Nitrophenylphosphorylcholine	37°C, pH 7.2	Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number	Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
3.1.4.3	17000	-	1 * 79000 + 1 * 17000, SDS-PAGE	Pseudomonas aeruginosa
3.1.4.3	79000	-	1 * 79000 + 1 * 17000, SDS-PAGE	Pseudomonas aeruginosa

Organism

EC Number	Organism	UniProt	Comment	Textmining
3.1.4.3	Pseudomonas aeruginosa	-	-	-

Purification (Commentary)

EC Number	Purification (Comment)	Organism
3.1.4.3	-	Pseudomonas aeruginosa

Specific Activity [micromol/min/mg]

EC Number	Specific Activity Minimum [µmol/min/mg]	Specific Activity Maximum [µmol/min/mg]	Comment	Organism
3.1.4.3	149.7	-	-	Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
3.1.4.3	p-nitrophenylphosphorylcholine + H2O	-	Pseudomonas aeruginosa	p-nitrophenol + phosphorylcholine	-	?
3.1.4.3	phosphatidylcholine + H2O	-	Pseudomonas aeruginosa	1,2-diacylglycerol + phosphorylcholine	-	?
3.1.4.3	plasmalogen + H2O	-	Pseudomonas aeruginosa	?	-	?
3.1.4.3	sphingomyelin + H2O	-	Pseudomonas aeruginosa	ceramide + phosphorylcholine	-	?

Subunits

EC Number	Subunits	Comment	Organism
3.1.4.3	dimer	1 * 79000 + 1 * 17000, SDS-PAGE	Pseudomonas aeruginosa

Synonyms

EC Number	Synonyms	Comment	Organism
3.1.4.3	PlcHR2	-	Pseudomonas aeruginosa

Turnover Number [1/s]

EC Number	Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
3.1.4.3	979	-	p-Nitrophenylphosphorylcholine	37°C, PH 7.2	Pseudomonas aeruginosa

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Release 2023.1 (January 2023)