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Literature summary extracted from

  • Teramoto, M.; Futamata, H.; Harayama, S.; Watanabe, K.
    Characterization of a high-affinity phenol hydroxylase from Comamonas testosteroni R5 by gene cloning, and expression in Pseudomonas aeruginosa PAO1c (1999), Mol. Gen. Genet., 262, 552-558.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.7 expression in Pseudomonas aeruginosa PAO1c Comamonas testosteroni

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.7 phenol + NADPH + O2 Comamonas testosteroni initial step in phenol-degrading pathway catechol + NADP+ + H2O
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?
1.14.13.7 phenol + NADPH + O2 Comamonas testosteroni R5 initial step in phenol-degrading pathway catechol + NADP+ + H2O
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?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Comamonas testosteroni
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1.14.13.7 Comamonas testosteroni R5
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Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 2-ethylphenol + NADPH + O2 18% of the activity with phenol Comamonas testosteroni 3-ethyl-benzene-1,2-diol + NADP+ + H2O
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?
1.14.13.7 2-ethylphenol + NADPH + O2 18% of the activity with phenol Comamonas testosteroni R5 3-ethyl-benzene-1,2-diol + NADP+ + H2O
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?
1.14.13.7 3,4-dimethylphenol + NADPH + O2 72% of the activity with phenol Comamonas testosteroni ?
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?
1.14.13.7 3,4-dimethylphenol + NADPH + O2 72% of the activity with phenol Comamonas testosteroni R5 ?
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?
1.14.13.7 benzene + NADPH + O2 26% of the activity with phenol Comamonas testosteroni ?
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?
1.14.13.7 m-chlorophenol + NADPH + O2 18% of the activity with phenol Comamonas testosteroni 4-chloro-benzene-1,2-diol + NADP+ + H2O
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?
1.14.13.7 m-cresol + NADPH + O2 114% of the activity with phenol Comamonas testosteroni ?
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?
1.14.13.7 o-chlorophenol + NADPH + O2 20% of the activity with phenol Comamonas testosteroni 3-chloro-benzene-1,2-diol + NADP+ + H2O
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?
1.14.13.7 o-cresol + NADPH + O2 37% of the activity with phenol, measured as substrate-dependent oxygen uptake rate by derivatives of Pseudomonas aeruginosa PAO1c carrying the enzyme genes after induction with phenol Comamonas testosteroni 3-methylcatechol + NADP+ + H2O
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?
1.14.13.7 orcinol + NADPH + O2 46% of the activity with phenol Comamonas testosteroni ?
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?
1.14.13.7 p-chlorophenol + NADPH + O2 84% of the activity with phenol Comamonas testosteroni ?
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?
1.14.13.7 p-cresol + NADPH + O2 114% of the activity with phenol Comamonas testosteroni 4-methylcatechol + NADP+ + H2O
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?
1.14.13.7 phenol + NADPH + O2
-
Comamonas testosteroni catechol + NADP+ + H2O
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?
1.14.13.7 phenol + NADPH + O2 initial step in phenol-degrading pathway Comamonas testosteroni catechol + NADP+ + H2O
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?
1.14.13.7 phenol + NADPH + O2
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Comamonas testosteroni R5 catechol + NADP+ + H2O
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?
1.14.13.7 phenol + NADPH + O2 initial step in phenol-degrading pathway Comamonas testosteroni R5 catechol + NADP+ + H2O
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?
1.14.13.7 quinol + NADPH + O2 63% of the activity with phenol Comamonas testosteroni ?
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?
1.14.13.7 resorcinol + NADPH + O2 27% of the activity with phenol Comamonas testosteroni ?
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?
1.14.13.7 resorcinol + NADPH + O2 27% of the activity with phenol Comamonas testosteroni R5 ?
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?
1.14.13.7 toluene + NADPH + O2 28% of the activity with phenol Comamonas testosteroni ?
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?