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Literature summary extracted from

  • Haider, N.; Eschbach, M.L.; Dias Sde, S.; Gilberger, T.W.; Walter, R.D.; Luersen, K.
    The spermidine synthase of the malaria parasite Plasmodium falciparum: molecular and biochemical characterisation of the polyamine synthesis enzyme (2005), Mol. Biochem. Parasitol., 142, 224-236.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.5.1.16 expression in Escherichia coli, without the first 29 amino acids of the N-terminal extension Plasmodium falciparum

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.16 1-Aminooxy-3-aminopropane poor inhibition Plasmodium falciparum
2.5.1.16 2-mercaptoethylamine poor inhibition Plasmodium falciparum
2.5.1.16 5'-methylthioadenosine feedback inhibition Plasmodium falciparum
2.5.1.16 5-amino-1-pentene 50% inhibition at 6.5 microM Plasmodium falciparum
2.5.1.16 Cyclohexylamine 50% inhibition at 19.7 microM Plasmodium falciparum
2.5.1.16 Dicyclohexylamine poor inhibition Plasmodium falciparum
2.5.1.16 additional information not inhibitory: S-adenosylmethioninamine concentrations up to 150 microM Plasmodium falciparum
2.5.1.16 trans-4-Methylcyclohexylamine IC50 1.4 microM Plasmodium falciparum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.16 0.0353
-
 S-adenosylmethioninamine pH 7.0, 37°C Plasmodium falciparum
2.5.1.16 0.052
-
 putrescine pH 7.0, 37°C Plasmodium falciparum

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.5.1.16 cytoplasm
-
 Plasmodium falciparum 5737
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.16 37000
-
 2 * 37000, SDS-PAGE Plasmodium falciparum
2.5.1.16 70000
-
 gel filtration Plasmodium falciparum

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.16 Plasmodium falciparum Q9NFS5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.16 metal affinity chromatography and gel filtration Plasmodium falciparum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.16 putrescine + S-adenosylmethioninamine
-
 Plasmodium falciparum spermidine + 5'-methylthioadenosine
-
 ?
2.5.1.16 spermidine + S-adenosylmethioninamine in contrast to mammalian spermidine synthases, spermidine can replace to some extent putrescine as the aminopropyl acceptor Plasmodium falciparum spermine + 5'-methylthioadenosine
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.16 dimer 2 * 37000, SDS-PAGE Plasmodium falciparum

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.5.1.16 0.00018
-
 trans-4-Methylcyclohexylamine
-
 Plasmodium falciparum

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.16 0.0014
-
 IC50 1.4 microM Plasmodium falciparum trans-4-Methylcyclohexylamine