BRENDA - Enzyme Database show

Human microsomal prostaglandin E synthase 1: a member of the MAPEG protein superfamily

Pettersson, P.L.; Thoren, S.; Jakobsson, P.J.; Methods Enzymol. 401, 147-161 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
glutathione
-
Homo sapiens
5.3.99.3
additional information
proinflammatory cytokinin, induction of the enzyme
Homo sapiens
Application
EC Number
Application
Commentary
Organism
5.3.99.3
drug development
the enzyme constitues an attractive drug target for treatment of inflammatory diseases
Homo sapiens
Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
additional information
glucocorticosteroids, down-regulation of the enzyme
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.16
-
prostaglandin G2
-
Homo sapiens
5.3.99.3
0.16
-
prostaglandin H2
-
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
215000
-
gradient centrifugation, density equilibrium centrifugation, gel filtration
Homo sapiens
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Homo sapiens
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
-
Homo sapiens
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
additional information
in patients with rheumatoid arthritis, the protein is overexpressed in synovial tissue, particularly by macrophages and fibroblasts in the synovial lining layer
Homo sapiens
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.3
100
200
in the presence of 0.4 mM prostaglandin H2 or prostaglandin G2 and 2.5 mM glutatione
Homo sapiens
5.3.99.3
510
-
in the presence of 10 microM prostaglandin H2 or prostaglandin G2 and 2.5 mM glutatione
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
prostaglandin G2
at pH 7.5, 2.5 mM glutathione and 10 microM substrate
662768
Homo sapiens
15-hydroperoxy-prostaglandin E2
-
-
-
?
5.3.99.3
Prostaglandin H2
at pH 7.5, 2.5 mM glutathione and 10 microM substrate
662768
Homo sapiens
Prostaglandin E2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
trimer
3 * ?, gradient centrifugation, density equilibrium centrifugation, gel filtration
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.3
50
-
prostaglandin H2
-
Homo sapiens
5.3.99.3
75
-
prostaglandin G2
-
Homo sapiens
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
5.3.99.3
glutathione
-
Homo sapiens
5.3.99.3
additional information
proinflammatory cytokinin, induction of the enzyme
Homo sapiens
Application (protein specific)
EC Number
Application
Commentary
Organism
5.3.99.3
drug development
the enzyme constitues an attractive drug target for treatment of inflammatory diseases
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.3
additional information
glucocorticosteroids, down-regulation of the enzyme
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.3
0.16
-
prostaglandin G2
-
Homo sapiens
5.3.99.3
0.16
-
prostaglandin H2
-
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.3
215000
-
gradient centrifugation, density equilibrium centrifugation, gel filtration
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
-
Homo sapiens
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
5.3.99.3
additional information
in patients with rheumatoid arthritis, the protein is overexpressed in synovial tissue, particularly by macrophages and fibroblasts in the synovial lining layer
Homo sapiens
-
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [Ámol/min/mg]
Specific Activity Maximum [Ámol/min/mg]
Commentary
Organism
5.3.99.3
100
200
in the presence of 0.4 mM prostaglandin H2 or prostaglandin G2 and 2.5 mM glutatione
Homo sapiens
5.3.99.3
510
-
in the presence of 10 microM prostaglandin H2 or prostaglandin G2 and 2.5 mM glutatione
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
prostaglandin G2
at pH 7.5, 2.5 mM glutathione and 10 microM substrate
662768
Homo sapiens
15-hydroperoxy-prostaglandin E2
-
-
-
?
5.3.99.3
Prostaglandin H2
at pH 7.5, 2.5 mM glutathione and 10 microM substrate
662768
Homo sapiens
Prostaglandin E2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
trimer
3 * ?, gradient centrifugation, density equilibrium centrifugation, gel filtration
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
5.3.99.3
50
-
prostaglandin H2
-
Homo sapiens
5.3.99.3
75
-
prostaglandin G2
-
Homo sapiens