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Literature summary extracted from

  • Ranjith-Kumar, C.T.; Sarisky, R.T.; Gutshall, L.; Thomson, M.; Kao, C.C.
    De novo initiation pocket mutations have multiple effects on hepatitis C virus RNA-dependent RNA polymerase activities (2004), J. Virol., 78, 12207-12217.
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
2.7.7.48 D225A mutant enzyme is capable of robust de novo initiation in the presence of Mn2+, produces reduced amounts of product of 38 nt and longer that arose from template switch Hepacivirus C
2.7.7.48 R158A mutation decreases de novo initiation of RNA synthesis Hepacivirus C
2.7.7.48 R158A/R367A/R386A mutation eliminates de novo initiation product even in presence of Mn2+, but enzyme is capable of primer extension Hepacivirus C
2.7.7.48 R158A/R386A mutation eliminates de novo initiation product even in presence of Mn2+, but enzyme is capable of primer extension Hepacivirus C
2.7.7.48 R158A/R394A mutation eliminates de novo initiation product even in presence of Mn2+, but enzyme is capable of primer extension Hepacivirus C
2.7.7.48 R386A mutation decreases de novo initiation of RNA synthesis Hepacivirus C
2.7.7.48 R394A mutation decreases de novo initiation of RNA synthesis Hepacivirus C
2.7.7.48 R48A mutation decreases de novo initiation of RNA synthesis Hepacivirus C

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.7.48 0.0035
-
 GTP pH 8.2, 25°C, enzyme form DELTA21 that lacks the C-terminal 21 hydrophobic residues Hepacivirus C
2.7.7.48 5.05
-
 GTP pH 8.2, 25°C, mutant enzyme Hepacivirus C
2.7.7.48 10.1
-
 GTP pH 8.2, 25°C, mutant enzyme Hepacivirus C
2.7.7.48 11.25
-
 GTP pH 8.2, 25°C, mutant enzyme Hepacivirus C
2.7.7.48 15.3
-
 GTP pH 8.2, 25°C, mutant enzyme R158A Hepacivirus C
2.7.7.48 25.9
-
 GTP pH 8.2, 25°C, mutant enzyme Hepacivirus C
2.7.7.48 33.8
-
 GTP pH 8.2, 25°C, mutant enzyme Hepacivirus C

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.48 Mn2+ likely helps to stabilize the interaction between the active site and the NTPi Hepacivirus C

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Hepacivirus C
-
 expression in Escherichia coli
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.48 enzyme expressed in Escherichia coli Hepacivirus C

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 GTP + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn
-
 Hepacivirus C diphosphate + RNAn+1
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 NS5B
-
 Hepacivirus C
2.7.7.48 RNA-dependent RNA polymerase
-
 Hepacivirus C