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Literature summary extracted from

  • Ishibashi, M.; Sakashita, K.; Tokunaga, H.; Arakawa, T.; Tokunaga, M.
    Activation of halophilic nucleoside diphosphate kinase by a non-ionic osmolyte, trimethylamine N-oxide (2003), J. Protein Chem., 22, 345-351.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.4.6 Trimethylamine N-oxide concentration of 4 M required for activity, at pH 8 inclusion of low concentrations of NaCl required for activation Halobacterium salinarum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.6 KCl concentration of 3 M required for activity Halobacterium salinarum
2.7.4.6 NaCl concentration of 2 M required for activity Halobacterium salinarum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.6 ATP + NDP Halobacterium salinarum contributes to the maintenance of the cellular pools of all nucleosides triphosphates ADP + NTP
-
 ?
2.7.4.6 ATP + NDP Halobacterium salinarum 4R contributes to the maintenance of the cellular pools of all nucleosides triphosphates ADP + NTP
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.6 Halobacterium salinarum
-
-
-
2.7.4.6 Halobacterium salinarum 4R
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.4.6
-
 Halobacterium salinarum

Renatured (Commentary)

EC Number Renatured (Comment) Organism
2.7.4.6 extremely halophilic enzyme inactivated at low salt concentrations using heat-treatment or 6 M urea, addition of or dialysis against 2 M NaCl, 3 M KCl, or 4 M trimethylamine N-oxide required for effective refolding of enzyme, refolding using 4 M trimethylamine N-oxide is strongly pH-dependent, good refolding at pH 7, almost no activity regained at pH 8, at pH 8 inclusion of low concentrations of NaCl supports refolding and activation of activity Halobacterium salinarum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.6 ATP + NDP
-
 Halobacterium salinarum ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates Halobacterium salinarum ADP + NTP
-
 ?
2.7.4.6 ATP + NDP
-
 Halobacterium salinarum 4R ADP + NTP
-
 ?
2.7.4.6 ATP + NDP contributes to the maintenance of the cellular pools of all nucleosides triphosphates Halobacterium salinarum 4R ADP + NTP
-
 ?