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Crystal structure and possible catalytic mechanism of microsomal prostaglandin E synthase type 2 (mPGES-2)

Yamada, T.; Komoto, J.; Watanabe, K.; Ohmiya, Y.; Takusagawa, F.; J. Mol. Biol. 348, 1163-1176 (2005)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli; overexpression of truncated mPGES-2, comprising residues 1-87, with an extended His-tag in Escherichia coli strain BL21 by nickel affinity chromatography
Macaca fascicularis
Crystallization (Commentary)
EC Number
Crystallization
Organism
5.3.99.3
purified recombinant His-tagged truncated mPGES-2, complexed with anti-inflammatory drug IMN, 10 mg/ml protein in 1.7 M ammonium sulfate, 0.1 M sodium acetate-HCl, pH 5.5, and 1.5 mM indomethacin, sitting drop vapour diffusion method, 3 days, 22°C, X-ray diffraction structure determinationand analysis at 2.6 A resolution, modeling; with an anti-inflammatory drug indomethacin, 2.6 A resolution
Macaca fascicularis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Macaca fascicularis
-
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Macaca fascicularis
-
Prostaglandin E2
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.3
Macaca fascicularis
Q9N0A4
-
-
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.3
; recombinant His-tagged truncated mPGES-2 from Escherichia coli strain BL21 by nickel affinity chromatography
Macaca fascicularis
Reaction
EC Number
Reaction
Commentary
Organism
5.3.99.3
(5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate
catalytic mechanism of mPGES-2, hydrogen bond-forming residues Y107, C113, C110, and F112 are involved, substrate binding and catalytic sites
Macaca fascicularis
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
662644
Macaca fascicularis
Prostaglandin E2
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
5.3.99.3
dimer
crystal structure
Macaca fascicularis
5.3.99.3
More
structure-function analysis, overview
Macaca fascicularis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
expression in Escherichia coli; overexpression of truncated mPGES-2, comprising residues 1-87, with an extended His-tag in Escherichia coli strain BL21 by nickel affinity chromatography
Macaca fascicularis
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
5.3.99.3
purified recombinant His-tagged truncated mPGES-2, complexed with anti-inflammatory drug IMN, 10 mg/ml protein in 1.7 M ammonium sulfate, 0.1 M sodium acetate-HCl, pH 5.5, and 1.5 mM indomethacin, sitting drop vapour diffusion method, 3 days, 22°C, X-ray diffraction structure determinationand analysis at 2.6 A resolution, modeling; with an anti-inflammatory drug indomethacin, 2.6 A resolution
Macaca fascicularis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.3
microsome
-
Macaca fascicularis
-
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.3
Prostaglandin H2
Macaca fascicularis
-
Prostaglandin E2
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.3
; recombinant His-tagged truncated mPGES-2 from Escherichia coli strain BL21 by nickel affinity chromatography
Macaca fascicularis
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.3
Prostaglandin H2
-
662644
Macaca fascicularis
Prostaglandin E2
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
5.3.99.3
dimer
crystal structure
Macaca fascicularis
5.3.99.3
More
structure-function analysis, overview
Macaca fascicularis