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Literature summary extracted from

  • Yamada, T.; Komoto, J.; Watanabe, K.; Ohmiya, Y.; Takusagawa, F.
    Crystal structure and possible catalytic mechanism of microsomal prostaglandin E synthase type 2 (mPGES-2) (2005), J. Mol. Biol., 348, 1163-1176.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
5.3.99.3 expression in Escherichia coli Macaca fascicularis
5.3.99.3 overexpression of truncated mPGES-2, comprising residues 1-87, with an extended His-tag in Escherichia coli strain BL21 by nickel affinity chromatography Macaca fascicularis

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
5.3.99.3 purified recombinant His-tagged truncated mPGES-2, complexed with anti-inflammatory drug IMN, 10 mg/ml protein in 1.7 M ammonium sulfate, 0.1 M sodium acetate-HCl, pH 5.5, and 1.5 mM indomethacin, sitting drop vapour diffusion method, 3 days, 22°C, X-ray diffraction structure determinationand analysis at 2.6 A resolution, modeling Macaca fascicularis
5.3.99.3 with an anti-inflammatory drug indomethacin, 2.6 A resolution Macaca fascicularis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.3.99.3 microsome
-
 Macaca fascicularis
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
5.3.99.3 Prostaglandin H2 Macaca fascicularis
-
 Prostaglandin E2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
5.3.99.3 Macaca fascicularis Q9N0A4
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
5.3.99.3
-
 Macaca fascicularis
5.3.99.3 recombinant His-tagged truncated mPGES-2 from Escherichia coli strain BL21 by nickel affinity chromatography Macaca fascicularis

Reaction

EC Number Reaction Comment Organism Reaction ID
5.3.99.3 (5Z,13E)-(15S)-9alpha,11alpha-epidioxy-15-hydroxyprosta-5,13-dienoate = (5Z,13E)-(15S)-11alpha,15-dihydroxy-9-oxoprosta-5,13-dienoate catalytic mechanism of mPGES-2, hydrogen bond-forming residues Y107, C113, C110, and F112 are involved, substrate binding and catalytic sites Macaca fascicularis
a

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.3.99.3 Prostaglandin H2
-
 Macaca fascicularis Prostaglandin E2
-
 ?

Subunits

EC Number Subunits Comment Organism
5.3.99.3 dimer crystal structure Macaca fascicularis
5.3.99.3 More structure-function analysis, overview Macaca fascicularis

Synonyms

EC Number Synonyms Comment Organism
5.3.99.3 microsomal prostaglandin E synthase type 2
-
 Macaca fascicularis
5.3.99.3 mPGES-2
-
 Macaca fascicularis
5.3.99.3 prostaglandin E synthase type 2
-
 Macaca fascicularis