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Literature summary extracted from

  • Christian, T.; Evilia, C.; Williams, S.; Hou, Y.M.
    Distinct origins of tRNA(m1G37) methyltransferase (2004), J. Mol. Biol., 339, 707-719.
    View publication on PubMed

Application

EC Number Application Comment Organism
2.1.1.228 medicine the sequence and structural distinction of bacterial TrmD from the eukaryotic Trm5 suggests that strategies can be developed to specifically inhibit TrmD without a deleterious effect on humans Methanocaldococcus jannaschii

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.1.1.228 expression in Escherichia coli Methanocaldococcus jannaschii

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.228 D201A 59% activity realtive to the wild-type Methanocaldococcus jannaschii
2.1.1.228 D223A 6.2% activity realtive to the wild-type Methanocaldococcus jannaschii
2.1.1.228 G205A/G207A 12.3% activity realtive to the wild-type Methanocaldococcus jannaschii
2.1.1.228 N265A 11.2% activity realtive to the wild-type Methanocaldococcus jannaschii
2.1.1.228 P267A 1.3% activity realtive to the wild-type Methanocaldococcus jannaschii

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.1.1.228 42000
-
 x * 42000, SDS-PAGE Methanocaldococcus jannaschii

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.228 Methanocaldococcus jannaschii Q58293
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.1.1.228
-
 Methanocaldococcus jannaschii

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.228 additional information the enzyme is specific for methylation of guanine37 in tRNA. No methylation of tRNAArg(UCU) possessing the sequence U36G37 Methanocaldococcus jannaschii ?
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAArg(UCG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAArg(UCG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNACys(GCA) possessing the sequence A36G37. The enzyme is inactive with mutant forms of Methanocaldococcus jannaschii tRNACys(GCA) containing A37, C37, or U37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNACys(GCA)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAGlu(UUC) possessing the sequence C36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAGlu(UUC)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNALeu(UCG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNALeu(UCG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro(GGG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro(GGG)
-
 ?
2.1.1.228 S-adenosyl-L-methionine + guanine37 in Methanocaldococcus jannaschii tRNAPro(UGG) possessing the sequence G36G37 Methanocaldococcus jannaschii S-adenosyl-L-homocysteine + N1-methylguanine37 in Methanocaldococcus jannaschii tRNAPro(UGG)
-
 ?

Subunits

EC Number Subunits Comment Organism
2.1.1.228 ? x * 42000, SDS-PAGE Methanocaldococcus jannaschii

Synonyms

EC Number Synonyms Comment Organism
2.1.1.228 MJ0883
-
 Methanocaldococcus jannaschii
2.1.1.228 tRNA(m1G37) methyltransferase
-
 Methanocaldococcus jannaschii
2.1.1.228 tRNA(m1G37) MTase
-
 Methanocaldococcus jannaschii

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.1.1.228 50
-
 assay at Methanocaldococcus jannaschii