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Literature summary extracted from

  • Knecht, W.; Petersen, G.E.; Munch-Petersen, B.; Piskur, J.
    Deoxyribonucleoside kinases belonging to the thymidine kinase 2 (TK2)-like group vary significantly in substrate specificity, kinetics and feed-back regulation (2002), J. Mol. Biol., 315, 529-540.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.1.145 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as C-terminally His8-tagged protein in Escherichia coli strain BL21 Bombyx mori
2.7.1.145 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression as full length and N-terminally truncated His-tagged enzymes in Escherichia coli Xenopus laevis
2.7.1.145 DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of wild-type and as C-terminally His8-tagged protein in Escherichia coli strain BL21 Drosophila melanogaster

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.145 dCTP competitive versus ATP, feedback inhibition Xenopus laevis
2.7.1.145 dTTP competitive versus ATP, feedback inhibition Bombyx mori
2.7.1.145 dTTP competitive versus ATP, feedback inhibition Drosophila melanogaster
2.7.1.145 dTTP competitive versus ATP, feedback inhibition Xenopus laevis
2.7.1.145 additional information no inhibition by dNTPs other than dTTP Bombyx mori
2.7.1.145 additional information no inhibition by dNTPs other than dTTP Drosophila melanogaster

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.145 additional information
-
 additional information kinetics Bombyx mori
2.7.1.145 additional information
-
 additional information kinetics Xenopus laevis
2.7.1.145 0.0014
-
 ATP
-
 Bombyx mori
2.7.1.145 0.0025
-
 ATP
-
 Xenopus laevis
2.7.1.145 0.0061
-
 2'-deoxycytidine
-
 Bombyx mori
2.7.1.145 0.0153
-
 2'-deoxythymidine
-
 Bombyx mori
2.7.1.145 0.031
-
 2'-deoxycytidine
-
 Xenopus laevis
2.7.1.145 0.138
-
 2'-deoxythymidine
-
 Xenopus laevis
2.7.1.145 0.429
-
 2'-deoxyguanosine
-
 Bombyx mori
2.7.1.145 0.501
-
 2'-deoxyadenosine
-
 Bombyx mori
2.7.1.145 1.2
-
 2'-deoxyguanosine
-
 Xenopus laevis
2.7.1.145 2.1
-
 2'-deoxyadenosine
-
 Xenopus laevis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.145 additional information no sorting signals in the amino acid sequence Bombyx mori
-
-
2.7.1.145 nucleus enzyme possesses 2 potential nuclear targeting sequences Drosophila melanogaster 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.145 Mg2+
-
 Bombyx mori
2.7.1.145 Mg2+
-
 Xenopus laevis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.145 26300
-
 2 * 26300, recombinant enzyme, SDS-PAGE Xenopus laevis
2.7.1.145 26900
-
 2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE Drosophila melanogaster
2.7.1.145 29100
-
 2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE Drosophila melanogaster
2.7.1.145 31300
-
 2 * 31300, recombinant enzyme, SDS-PAGE Bombyx mori
2.7.1.145 44600
-
 recombinant N-terminally truncated enzyme, gel filtration Drosophila melanogaster
2.7.1.145 46000
-
 recombinant enzyme, native PAGE Xenopus laevis
2.7.1.145 48500
-
 recombinant full length enzyme, gel filtration Drosophila melanogaster
2.7.1.145 49000
-
 recombinant N-terminally truncated enzyme, native PAGE Drosophila melanogaster
2.7.1.145 49800
-
 recombinant enzyme, gel filtration Xenopus laevis
2.7.1.145 57700
-
 recombinant full length enzyme, native PAGE Drosophila melanogaster
2.7.1.145 64400
-
 recombinant enzyme, gel filtration Bombyx mori
2.7.1.145 69300
-
 recombinant enzyme, native PAGE Bombyx mori

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.145 ATP + 2'-deoxycytidine Xenopus laevis
-
 ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine Xenopus laevis
-
 ADP + 2'-deoxythymidine 5'-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.145 Bombyx mori Q9BKL3 putative
-
2.7.1.145 Drosophila melanogaster
-
-
-
2.7.1.145 Xenopus laevis Q8UVZ9 putative
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.145 recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography Bombyx mori
2.7.1.145 recombinant C-terminally His8-tagged enzyme from Escherichia coli strain BL21 by nickel affinity chromatography, the His-tag is cleaved off by thrombin Drosophila melanogaster
2.7.1.145 recombinant full length and N-terminally truncated His-tagged enzymes, the His-tags are cleaved off Xenopus laevis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.7.1.145 brain
-
 Xenopus laevis
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.145 additional information
-
 substrate specificity Bombyx mori
2.7.1.145 additional information
-
 substrate specificity Xenopus laevis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.145 ATP + 1-beta-D-arabinofuranosylcytosine
-
 Bombyx mori ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 1-beta-D-arabinofuranosylcytosine
-
 Xenopus laevis ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 1-beta-D-arabinofuranosylthymine
-
 Bombyx mori ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
 ?
2.7.1.145 ATP + 1-beta-D-arabinofuranosylthymine
-
 Xenopus laevis ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2',3'-didehydro-3'-deoxythymidine
-
 Bombyx mori ADP + 2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2',3'-dideoxycytidine
-
 Bombyx mori ADP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2',3'-dideoxycytidine low activity Xenopus laevis ADP + 2',3'-dideoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2',3'-dideoxythymidine
-
 Bombyx mori ADP + 2',3'-dideoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2',3'-dideoxythymidine low activity Xenopus laevis ADP + 2',3'-dideoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Bombyx mori ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyadenosine
-
 Xenopus laevis ADP + 2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Bombyx mori ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxycytidine
-
 Xenopus laevis ADP + 2'-deoxycytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine
-
 Bombyx mori ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxyguanosine
-
 Xenopus laevis ADP + 2'-deoxyguanosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine
-
 Xenopus laevis ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine best substrate Xenopus laevis ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2'-deoxythymidine high activity Bombyx mori ADP + 2'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2-chloro-2'-deoxyadenosine low activity Xenopus laevis ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 2-chloro-2'-deoxyadenosine high activity Bombyx mori ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine low activity Bombyx mori ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 3'-azido-2',3'-didehydro-3'-deoxythymidine low activity Xenopus laevis ADP + 3'-azido-2',3'-didehydro-3'-deoxythymidine 5'-phosphate
-
 ?
2.7.1.145 ATP + 3'-deoxyadenosine
-
 Bombyx mori ADP + 3'-deoxyadenosine 5'-phosphate
-
 ?
2.7.1.145 ATP + 5-fluoro-2'-deoxyuridine high activity Bombyx mori ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.145 ATP + 5-fluoro-2'-deoxyuridine high activity Xenopus laevis ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate
-
 ?
2.7.1.145 ATP + 9-beta-D-arabinofuranosyladenine
-
 Bombyx mori ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
 ?
2.7.1.145 ATP + 9-beta-D-arabinofuranosyladenine very low activity Xenopus laevis ADP + 9-beta-D-arabinofuranosyladenine 5'-phosphate
-
 ?
2.7.1.145 ATP + aciclovir
-
 Bombyx mori ADP + aciclovir phosphate
-
 ?
2.7.1.145 ATP + cytidine
-
 Bombyx mori ADP + cytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + cytidine very low activity Xenopus laevis ADP + cytidine 5'-phosphate
-
 ?
2.7.1.145 ATP + deoxyuridine best substrate Bombyx mori ADP + deoxyuridine 5'-phosphate
-
 ?
2.7.1.145 ATP + deoxyuridine high activity Xenopus laevis ADP + deoxyuridine 5'-phosphate
-
 ?
2.7.1.145 ATP + fludarabine
-
 Bombyx mori ADP + fludarabine phosphate
-
 ?
2.7.1.145 ATP + stavudine anti-HIV nucleoside analogue Bombyx mori ADP + stavudine phosphate
-
 ?
2.7.1.145 ATP + uridine very low activity Xenopus laevis ADP + uridine 5'-phosphate
-
 ?
2.7.1.145 ATP + uridine low activity Bombyx mori ADP + uridine 5'-phosphate
-
 ?
2.7.1.145 additional information substrate specificity Drosophila melanogaster ?
-
 ?
2.7.1.145 additional information substrate specificity, no activity with 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine Bombyx mori ?
-
 ?
2.7.1.145 additional information substrate specificity, no activity with fludarabine, 3'-deoxyadenosine, 2',3'-didehydro-3'-deoxythymidine, 2-deoxyribose, dideoxyadenosine, dideoxyguanosine, adenosine, and guanosine Xenopus laevis ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.145 dimer 2 * 26300, recombinant enzyme, SDS-PAGE Xenopus laevis
2.7.1.145 dimer 2 * 29100, recombinant full length enzyme, SDS-PAGE, 2 * 26900, recombinant N-terminally truncated enzyme, SDS-PAGE Drosophila melanogaster
2.7.1.145 dimer 2 * 31300, recombinant enzyme, SDS-PAGE Bombyx mori
2.7.1.145 More quarternary structure determination Bombyx mori
2.7.1.145 More quarternary structure determination Xenopus laevis

Synonyms

EC Number Synonyms Comment Organism
2.7.1.145 dNK
-
 Drosophila melanogaster
2.7.1.145 dNK
-
 Bombyx mori
2.7.1.145 More the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes Drosophila melanogaster
2.7.1.145 More the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes Bombyx mori
2.7.1.145 More the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes Xenopus laevis
2.7.1.145 multisubstrate deoxyribonucleoside kinase
-
 Drosophila melanogaster
2.7.1.145 multisubstrate deoxyribonucleoside kinase
-
 Bombyx mori
2.7.1.145 pyrimidine deoxyribonucleoside kinase
-
 Xenopus laevis
2.7.1.145 Xen-PyK
-
 Xenopus laevis

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.145 ATP
-
 Bombyx mori
2.7.1.145 ATP
-
 Xenopus laevis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.7.1.145 additional information
-
 additional information inhibition kinetics, overview Drosophila melanogaster
2.7.1.145 additional information
-
 additional information inhibition kinetics, overview Bombyx mori
2.7.1.145 additional information
-
 additional information inhibition kinetics, overview Xenopus laevis