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Literature summary extracted from

  • Choi, J.J.; Kwon, S.T.
    Cloning, expression, and characterization of DNA polymerase from hyperthermophilic bacterium Aquifex pyrophilus (2004), J. Microbiol. Biotechnol., 14, 1022-1030.
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.7.7
-
 Aquifex pyrophilus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.7.7 KCl optimal concentration is 20 mM Aquifex pyrophilus
2.7.7.7 Mg2+ optimal concentration is 3 mM Aquifex pyrophilus

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.7 Aquifex pyrophilus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.7.7
-
 Aquifex pyrophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.7 deoxynucleoside triphosphate + DNAn the enzyme contains a double strand-dependent 3'-5' proofreading exonuclease activity, but lacks any 5'-3' exonuclease activity Aquifex pyrophilus diphosphate + DNAn+1
-
 ?

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.7.7 7.5
-
-
 Aquifex pyrophilus