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Literature summary extracted from

  • Nakao, H.; Ishii, M.; Shinoda, S.; Yamamoto, S.
    Purification and some properties of a novel L-2,4-diaminobutyric acid decarboxylase from Vibrio alginolyticus (1989), J. Gen. Microbiol., 135, 345-351.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
4.1.1.86 additional information L-2,4-diaminobutanoate induces enzyme expression Vibrio alginolyticus

General Stability

EC Number General Stability Organism
4.1.1.86 freezing and thawing of the purified enzyme causes almost complete loss of activity Vibrio alginolyticus
4.1.1.86 pyridoxal 5'-phosphate is required for stability during storage Vibrio alginolyticus

Inhibitors

EC Number Inhibitors Comment Organism Structure
4.1.1.86 Ag+ over 90% inhibition at 1 mM Vibrio alginolyticus
4.1.1.86 Ca2+ 10% inhibition at 1 mM Vibrio alginolyticus
4.1.1.86 Fe2+ over 90% inhibition at 1 mM Vibrio alginolyticus
4.1.1.86 Hg2+ over 90% inhibition at 1 mM Vibrio alginolyticus
4.1.1.86 additional information the enzyme is not affected by GTP, ATP, DTT, Na+ or K+ Vibrio alginolyticus
4.1.1.86 propane-1,3-diamine product inhibition, about 50% at 5 mM Vibrio alginolyticus
4.1.1.86 Zn2+ over 90% inhibition at 1 mM Vibrio alginolyticus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
4.1.1.86 0.0056
-
 pyridoxal 5'-phosphate pH 8.3, 37°C Vibrio alginolyticus
4.1.1.86 0.081
-
 L-2,4-diaminobutanoate pH 8.3, 37°C Vibrio alginolyticus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.1.1.86 Mg2+ stimulates 30% at 20 mM Vibrio alginolyticus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
4.1.1.86 109000
-
 4 * 109000, SDS-PAGE Vibrio alginolyticus
4.1.1.86 450000
-
 gel filtration Vibrio alginolyticus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate Vibrio alginolyticus propane-1,3-diamine is a precursor for norspermidine, the enzyme is involved in biosynthesis of norspermidine propane-1,3-diamine + CO2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.1.1.86 Vibrio alginolyticus
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
4.1.1.86 3660fold to homogeneity by ammonium sulfate fractionation, gel filtration, and two steps of ion exchange chromatography Vibrio alginolyticus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
4.1.1.86 4.21
-
 purified enzyme Vibrio alginolyticus

Storage Stability

EC Number Storage Stability Organism
4.1.1.86 4°C, purified enzyme, pH 7.5, in presence of pyridoxal 5'-phosphate and NaN3, 3 weeks, stable Vibrio alginolyticus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.1.1.86 L-2,4-diaminobutanoate
-
 Vibrio alginolyticus propane-1,3-diamine + CO2
-
 ?
4.1.1.86 L-2,4-diaminobutanoate propane-1,3-diamine is a precursor for norspermidine, the enzyme is involved in biosynthesis of norspermidine Vibrio alginolyticus propane-1,3-diamine + CO2
-
 ?
4.1.1.86 additional information no activity with 2,3-diaminopropionate, ornithine, lysine, or arginine Vibrio alginolyticus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
4.1.1.86 tetramer 4 * 109000, SDS-PAGE Vibrio alginolyticus

Synonyms

EC Number Synonyms Comment Organism
4.1.1.86 DABA decarboxylase
-
 Vibrio alginolyticus
4.1.1.86 L-2,4-diaminobutyric acid decarboxylase
-
 Vibrio alginolyticus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
4.1.1.86 7.5 8.8 broad optimum Vibrio alginolyticus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
4.1.1.86 5.8 10 about half maximal activity at pH 6.5 and pH 9.3 Vibrio alginolyticus

Cofactor

EC Number Cofactor Comment Organism Structure
4.1.1.86 pyridoxal 5'-phosphate required, depletion leads to over 25% irreversible loss of activity Vibrio alginolyticus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
4.1.1.86 Vibrio alginolyticus isoelectric focusing
-
 6.8