Literature summary extracted from
Nakao, H.; Ishii, M.; Shinoda, S.; Yamamoto, S.
Purification and some properties of a novel L-2,4-diaminobutyric acid decarboxylase from Vibrio alginolyticus (1989), J. Gen. Microbiol., 135, 345-351.
Activating Compound
EC Number |
Activating Compound |
Comment |
Organism |
Structure |
---|
4.1.1.86 |
additional information |
L-2,4-diaminobutanoate induces enzyme expression |
Vibrio alginolyticus |
|
General Stability
EC Number |
General Stability |
Organism |
---|
4.1.1.86 |
freezing and thawing of the purified enzyme causes almost complete loss of activity |
Vibrio alginolyticus |
4.1.1.86 |
pyridoxal 5'-phosphate is required for stability during storage |
Vibrio alginolyticus |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
4.1.1.86 |
Ag+ |
over 90% inhibition at 1 mM |
Vibrio alginolyticus |
|
4.1.1.86 |
Ca2+ |
10% inhibition at 1 mM |
Vibrio alginolyticus |
|
4.1.1.86 |
Fe2+ |
over 90% inhibition at 1 mM |
Vibrio alginolyticus |
|
4.1.1.86 |
Hg2+ |
over 90% inhibition at 1 mM |
Vibrio alginolyticus |
|
4.1.1.86 |
additional information |
the enzyme is not affected by GTP, ATP, DTT, Na+ or K+ |
Vibrio alginolyticus |
|
4.1.1.86 |
propane-1,3-diamine |
product inhibition, about 50% at 5 mM |
Vibrio alginolyticus |
|
4.1.1.86 |
Zn2+ |
over 90% inhibition at 1 mM |
Vibrio alginolyticus |
|
KM Value [mM]
EC Number |
KM Value [mM] |
KM Value Maximum [mM] |
Substrate |
Comment |
Organism |
Structure |
---|
4.1.1.86 |
0.0056 |
- |
pyridoxal 5'-phosphate |
pH 8.3, 37°C |
Vibrio alginolyticus |
|
4.1.1.86 |
0.081 |
- |
L-2,4-diaminobutanoate |
pH 8.3, 37°C |
Vibrio alginolyticus |
|
Metals/Ions
EC Number |
Metals/Ions |
Comment |
Organism |
Structure |
---|
4.1.1.86 |
Mg2+ |
stimulates 30% at 20 mM |
Vibrio alginolyticus |
|
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
4.1.1.86 |
109000 |
- |
4 * 109000, SDS-PAGE |
Vibrio alginolyticus |
4.1.1.86 |
450000 |
- |
gel filtration |
Vibrio alginolyticus |
Natural Substrates/ Products (Substrates)
EC Number |
Natural Substrates |
Organism |
Comment (Nat. Sub.) |
Natural Products |
Comment (Nat. Pro.) |
Rev. |
Reac. |
---|
4.1.1.86 |
L-2,4-diaminobutanoate |
Vibrio alginolyticus |
propane-1,3-diamine is a precursor for norspermidine, the enzyme is involved in biosynthesis of norspermidine |
propane-1,3-diamine + CO2 |
- |
? |
|
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
4.1.1.86 |
Vibrio alginolyticus |
- |
- |
- |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
4.1.1.86 |
3660fold to homogeneity by ammonium sulfate fractionation, gel filtration, and two steps of ion exchange chromatography |
Vibrio alginolyticus |
Specific Activity [micromol/min/mg]
EC Number |
Specific Activity Minimum [µmol/min/mg] |
Specific Activity Maximum [µmol/min/mg] |
Comment |
Organism |
---|
4.1.1.86 |
4.21 |
- |
purified enzyme |
Vibrio alginolyticus |
Storage Stability
EC Number |
Storage Stability |
Organism |
---|
4.1.1.86 |
4°C, purified enzyme, pH 7.5, in presence of pyridoxal 5'-phosphate and NaN3, 3 weeks, stable |
Vibrio alginolyticus |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
4.1.1.86 |
L-2,4-diaminobutanoate |
- |
Vibrio alginolyticus |
propane-1,3-diamine + CO2 |
- |
? |
|
4.1.1.86 |
L-2,4-diaminobutanoate |
propane-1,3-diamine is a precursor for norspermidine, the enzyme is involved in biosynthesis of norspermidine |
Vibrio alginolyticus |
propane-1,3-diamine + CO2 |
- |
? |
|
4.1.1.86 |
additional information |
no activity with 2,3-diaminopropionate, ornithine, lysine, or arginine |
Vibrio alginolyticus |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
4.1.1.86 |
tetramer |
4 * 109000, SDS-PAGE |
Vibrio alginolyticus |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
4.1.1.86 |
DABA decarboxylase |
- |
Vibrio alginolyticus |
4.1.1.86 |
L-2,4-diaminobutyric acid decarboxylase |
- |
Vibrio alginolyticus |
pH Optimum
EC Number |
pH Optimum Minimum |
pH Optimum Maximum |
Comment |
Organism |
---|
4.1.1.86 |
7.5 |
8.8 |
broad optimum |
Vibrio alginolyticus |
pH Range
EC Number |
pH Minimum |
pH Maximum |
Comment |
Organism |
---|
4.1.1.86 |
5.8 |
10 |
about half maximal activity at pH 6.5 and pH 9.3 |
Vibrio alginolyticus |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
4.1.1.86 |
pyridoxal 5'-phosphate |
required, depletion leads to over 25% irreversible loss of activity |
Vibrio alginolyticus |
|
pI Value
EC Number |
Organism |
Comment |
pI Value Maximum |
pI Value |
---|
4.1.1.86 |
Vibrio alginolyticus |
isoelectric focusing |
- |
6.8 |