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Literature summary extracted from

  • Singh, U.; Panchanadikar, V.; Sarkar, D.
    Development of a simple assay protocol for high-throughput screening of Mycobacterium tuberculosis glutamine synthetase for the identification of novel inhibitors (2005), J. Biomol. Screen., 10, 725-729.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
6.3.1.2 L-methionine S-sulfoximine IC50: 3 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.1.2 0.002
-
 ADP pH 7.0 Mycobacterium tuberculosis
6.3.1.2 8.3
-
 hydroxylamine pH 7.0 Mycobacterium tuberculosis
6.3.1.2 60
-
 L-Gln pH 7.0 Mycobacterium tuberculosis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
6.3.1.2 Mn2+ Km: 0.025 mM Mycobacterium tuberculosis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
6.3.1.2 additional information Mycobacterium tuberculosis essential enzyme involved in the pathogenicity of Mycobacterium tuberculosis ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
6.3.1.2 Mycobacterium tuberculosis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.1.2 recombinant enzyme Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.1.2 L-Gln + hydroxylamine + ADP
-
 Mycobacterium tuberculosis gamma-glutamylhydroxamate + NH4+ + ?
-
 ?
6.3.1.2 additional information essential enzyme involved in the pathogenicity of Mycobacterium tuberculosis Mycobacterium tuberculosis ?
-
 ?

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
6.3.1.2 25
-
 pH 7.0, 0.002 mM ADP, 0.005 mM arsenate, 0.025 mM Mn2+, 60 mM L-glutamine, 8.3 mM hydroxylamine, stable for at least 3 h Mycobacterium tuberculosis
6.3.1.2 37
-
 pH 7.0, 0.002 mM ADP, 0.005 mM arsenate, 0.025 mM Mn2+, 60 mM L-glutamine, 8.3 mM hydroxylamine, stable for at least 3 h Mycobacterium tuberculosis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
6.3.1.2 3
-
 IC50: 3 mM Mycobacterium tuberculosis L-methionine S-sulfoximine