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Literature summary extracted from

  • Ogino, T.; Kobayashi, M.; Iwama, M.; Mizumoto, K.
    Sendai virus RNA-dependent RNA polymerase L protein catalyzes cap methylation of virus-specific mRNA (2005), J. Biol. Chem., 280, 4429-4435.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.7.48 Mg2+
-
 Murine respirovirus
2.7.7.48 Na+
-
 Murine respirovirus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn Murine respirovirus the enzyme catalyzes cap methylation of virus-specific mRNA as well as RNA synthesis diphosphate + RNAn+1
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.7.7.48 Murine respirovirus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.7.48 nucleoside triphosphate + RNAn the enzyme catalyzes cap methylation of virus-specific mRNA as well as RNA synthesis Murine respirovirus diphosphate + RNAn+1
-
 ?
2.7.7.48 nucleoside triphosphate + RNAn viral ribonucleoprotein complexes and purified recombinant L protein but not P protein exhibit mRNA (guanine-7-)methyl-transferase activity. mRNA synthesis in a reconstituted transcription system using purified N protein-genomic RNA complex as a template requires both the L and P proteins. Enzymatic properties of Senda virus mRNA (guanine-7-)methyl-transferase are different to that of cellular mRNA (guanine-7-)methyl-transferase. Unlike cellular enzyme, the SeV enzyme preferentially methylates capped RNA containing the viral mRNA 5'-end sequences (GpppApGpG-). The C-terminal part (amino acid residues 1,756–2,228) of the L protein catalyzes cap methylation, whereas the N-terminal half (residues 1–1,120) containing putative RNA polymerase subdomains does not Murine respirovirus diphosphate + RNAn+1
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.7.48 More RNA-dependent RNA polymerase complex, which consists of L protein (250000 Da) and P proteins (80000 Da) Murine respirovirus

Synonyms

EC Number Synonyms Comment Organism
2.7.7.48 RNA-dependent RNA polymerase
-
 Murine respirovirus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.7.48 30
-
-
 Murine respirovirus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.7.48 30 45 30°C: optimum, 45°C: 50-80% of maximal activity Murine respirovirus