BRENDA - Enzyme Database show

Identification of six novel allosteric effectors of Arabidopsis thaliana aspartate kinase-homoserine dehydrogenase isoforms. Physiological context sets the specificity

Curien, G.; Ravanel, S.; Robert, M.; Dumas, R.; J. Biol. Chem. 280, 41178-41183 (2005)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.2.4
L-alanine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-cysteine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-isoleucine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-leucine
4fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-serine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-valine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
Cloned(Commentary)
EC Number
Commentary
Organism
1.1.1.3
overexpression of AK-HSDH isoform 1 in Escherichia coli
Arabidopsis thaliana
2.7.2.4
expression in Escherichia coli
Arabidopsis thaliana
General Stability
EC Number
General Stability
Organism
2.7.2.4
pure protein , quickly frozen in N2 can be stored at -80C for several months without any loss of activity
Arabidopsis thaliana
2.7.2.4
pure protein, quickly frozen in N2 can be stored at -80C for several months without any loss of activity
Arabidopsis thaliana
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
Thr
-
Arabidopsis thaliana
2.7.2.4
L-leucine
allosteric effector
Arabidopsis thaliana
2.7.2.4
L-threonine
in the presence of 1 mM aspartate and 2 mM ATP
Arabidopsis thaliana
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.2.4
0.42
-
ATP
50 mM aspartate, presence of alanine
Arabidopsis thaliana
2.7.2.4
0.48
-
ATP
50 mM aspartate, presence of alanine
Arabidopsis thaliana
2.7.2.4
1.5
-
L-aspartate
20 mM ATP, presence of alanine
Arabidopsis thaliana
2.7.2.4
2.2
-
ATP
50 mM aspartate, absence of alanine
Arabidopsis thaliana
2.7.2.4
2.3
-
L-aspartate
20 mM ATP, presence of alanine
Arabidopsis thaliana
2.7.2.4
2.6
-
L-aspartate
20 mM ATP, absence of alanine
Arabidopsis thaliana
2.7.2.4
6.15
-
L-aspartate
20 mM ATP, absence of alanine
Arabidopsis thaliana
2.7.2.4
6.5
-
ATP
50 mM aspartate, absence of alanine
Arabidopsis thaliana
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.2.4
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.2.4
ATP + L-aspartate
Arabidopsis thaliana
-
ADP + 4-phospho-L-aspartate
-
-
?
2.7.2.4
ATP + L-aspartate
Arabidopsis thaliana
-
ADP + phospho-L-aspartate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.1.1.3
Arabidopsis thaliana
-
-
-
2.7.2.4
Arabidopsis thaliana
-
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.1.1.3
recombinant enzyme
Arabidopsis thaliana
2.7.2.4
-
Arabidopsis thaliana
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.2.4
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NADPH
-
662432
Arabidopsis thaliana
L-homoserine + NADP+
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
662432
Arabidopsis thaliana
ADP + 4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
662432
Arabidopsis thaliana
ADP + phospho-L-aspartate
-
-
-
?
Ki Value [mM]
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.2.4
0.049
-
L-threonine
in the presence of 1 mM aspartate and 2 mM ATP
Arabidopsis thaliana
2.7.2.4
0.091
-
L-threonine
in the presence of 1 mM aspartate and 2 mM ATP
Arabidopsis thaliana
2.7.2.4
1.1
-
L-leucine
-
Arabidopsis thaliana
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
2.7.2.4
L-alanine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-cysteine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-isoleucine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-leucine
4fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-serine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
2.7.2.4
L-valine
13fold activation at 2.5 mM; 3fold activation at 2.5 mM
Arabidopsis thaliana
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
overexpression of AK-HSDH isoform 1 in Escherichia coli
Arabidopsis thaliana
2.7.2.4
expression in Escherichia coli
Arabidopsis thaliana
General Stability (protein specific)
EC Number
General Stability
Organism
2.7.2.4
pure protein , quickly frozen in N2 can be stored at -80C for several months without any loss of activity
Arabidopsis thaliana
2.7.2.4
pure protein, quickly frozen in N2 can be stored at -80C for several months without any loss of activity
Arabidopsis thaliana
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.1.1.3
Thr
-
Arabidopsis thaliana
2.7.2.4
L-leucine
allosteric effector
Arabidopsis thaliana
2.7.2.4
L-threonine
in the presence of 1 mM aspartate and 2 mM ATP
Arabidopsis thaliana
Ki Value [mM] (protein specific)
EC Number
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
2.7.2.4
0.049
-
L-threonine
in the presence of 1 mM aspartate and 2 mM ATP
Arabidopsis thaliana
2.7.2.4
0.091
-
L-threonine
in the presence of 1 mM aspartate and 2 mM ATP
Arabidopsis thaliana
2.7.2.4
1.1
-
L-leucine
-
Arabidopsis thaliana
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.2.4
0.42
-
ATP
50 mM aspartate, presence of alanine
Arabidopsis thaliana
2.7.2.4
0.48
-
ATP
50 mM aspartate, presence of alanine
Arabidopsis thaliana
2.7.2.4
1.5
-
L-aspartate
20 mM ATP, presence of alanine
Arabidopsis thaliana
2.7.2.4
2.2
-
ATP
50 mM aspartate, absence of alanine
Arabidopsis thaliana
2.7.2.4
2.3
-
L-aspartate
20 mM ATP, presence of alanine
Arabidopsis thaliana
2.7.2.4
2.6
-
L-aspartate
20 mM ATP, absence of alanine
Arabidopsis thaliana
2.7.2.4
6.15
-
L-aspartate
20 mM ATP, absence of alanine
Arabidopsis thaliana
2.7.2.4
6.5
-
ATP
50 mM aspartate, absence of alanine
Arabidopsis thaliana
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
2.7.2.4
chloroplast
-
Arabidopsis thaliana
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.2.4
ATP + L-aspartate
Arabidopsis thaliana
-
ADP + 4-phospho-L-aspartate
-
-
?
2.7.2.4
ATP + L-aspartate
Arabidopsis thaliana
-
ADP + phospho-L-aspartate
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.1.1.3
recombinant enzyme
Arabidopsis thaliana
2.7.2.4
-
Arabidopsis thaliana
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
2.7.2.4
leaf
-
Arabidopsis thaliana
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.1.1.3
L-aspartate 4-semialdehyde + NADPH
-
662432
Arabidopsis thaliana
L-homoserine + NADP+
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
662432
Arabidopsis thaliana
ADP + 4-phospho-L-aspartate
-
-
-
?
2.7.2.4
ATP + L-aspartate
-
662432
Arabidopsis thaliana
ADP + phospho-L-aspartate
-
-
-
?