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Literature summary extracted from

  • Fukunaga, R.; Yokoyama, S.
    Structural basis for non-cognate amino acid discrimination by the valyl-tRNA synthetase editing domain (2005), J. Biol. Chem., 280, 29937-29945.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
6.1.1.9 1.7 A resolution crystal structure of the ValRS editing domain and 1.7 A resolution crystal structure of the editing domain bound with [N-(L-threonyl)-sulfamoyl]adenosine, hanging drop vapor diffusion method Thermus thermophilus

Protein Variants

EC Number Protein Variants Comment Organism
6.1.1.9 D276A valylation activity is similar to that of wild-type enzyme Thermus thermophilus
6.1.1.9 D279A valylation activity is similar to that of wild-type enzyme, efficiently produces Thr-tRNAVal, drastic decrease in ATP consumption rate, severe deficiency in deacylation activity with Thr-tRNAVal compared to wild-type enzyme Thermus thermophilus
6.1.1.9 F264A valylation activity is similar to that of wild-type enzyme, about 15% decrease in ATP consumption rate compared to wild-type enzyme, somewhat reduced deacylation activityith Thr-tRNAVal, mutation impairs editing activity of ValRS Thermus thermophilus
6.1.1.9 K270A slight reduction of valylation activity, efficiently produces Thr-tRNAVal, drastic decrease in ATP consumption rate, severe deficiency in deacylation activity with Thr-tRNAVal compared to wild-type enzyme Thermus thermophilus
6.1.1.9 R216A valylation activity is similar to that of wild-type enzyme, about 15% decrease in ATP consumption rate compared to wild-type enzyme, somewhat reduced deacylation activity with Thr-tRNAVal Thermus thermophilus
6.1.1.9 T272A valylation activity is similar to that of wild-type enzyme, about 15% decrease in ATP consumption rate compared to wild-type enzyme, somewhat reduced deacylation activity with Thr-tRNAVal Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
6.1.1.9 Thermus thermophilus P96142
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.1.1.9
-
Thermus thermophilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.1.1.9 ATP + L-valine + tRNAVal
-
Thermus thermophilus AMP + diphosphate + L-valyl-tRNAVal
-
?

Synonyms

EC Number Synonyms Comment Organism
6.1.1.9 ValRS
-
Thermus thermophilus