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Literature summary extracted from

  • Watanabe, K.; Nureki, O.; Fukai, S.; Ishii, R.; Okamoto, H.; Yokoyama, S.; Endo, Y.; Hori, H.
    Roles of conserved amino acid sequence motifs in the SpoU (TrmH) RNA methyltransferase family (2005), J. Biol. Chem., 280, 10368-10377.
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
2.1.1.34 E124A extensive structural alterations compared to wild-type, marked decrease in activity Thermus thermophilus
2.1.1.34 E124D small increase in Km-value Thermus thermophilus
2.1.1.34 E124Q no enzymic activity Thermus thermophilus
2.1.1.34 N152A extensive structural alterations compared to wild-type, marked decrease in activity Thermus thermophilus
2.1.1.34 N152D no enzymic activity Thermus thermophilus
2.1.1.34 N152E no enzymic activity Thermus thermophilus
2.1.1.34 N35A marked decrease in methyl transfer activity Thermus thermophilus
2.1.1.34 N35D marked decrease in Km-value for tRNA, low affinity for S-adenosyl-L-homocysteine Thermus thermophilus
2.1.1.34 N35Q dramatic increase in Km-value Thermus thermophilus
2.1.1.34 P143Q small increase in Km-value Thermus thermophilus
2.1.1.34 R41M large increase in Km-value Thermus thermophilus
2.1.1.34 S150C Km-value similar to wild-type, decrease in Vmax Thermus thermophilus
2.1.1.34 S150T 4fold increase in Km-value Thermus thermophilus
2.1.1.34 S37G 5fold increase in Km-value Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.1.1.34 0.01
-
 S-adenosyl-L-methionine pH 7.5, 50°C, wild-type Thermus thermophilus
2.1.1.34 0.011
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant S150C Thermus thermophilus
2.1.1.34 0.014
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant E124D Thermus thermophilus
2.1.1.34 0.014
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant P143Q Thermus thermophilus
2.1.1.34 0.037
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant S150T Thermus thermophilus
2.1.1.34 0.051
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant S37G Thermus thermophilus
2.1.1.34 0.083
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant N35A Thermus thermophilus
2.1.1.34 0.67
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant N35D Thermus thermophilus
2.1.1.34 0.92
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant R41M Thermus thermophilus
2.1.1.34 2.1
-
 S-adenosyl-L-methionine pH 7.5, 50°C, mutant N35Q Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.1.1.34 Thermus thermophilus
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.1.1.34 additional information enzyme hydroxylates guanosine at postion 18 in tRNA Thermus thermophilus ?
-
 ?
2.1.1.34 S-adenosyl-L-methionine + yeast tRNA-Phe
-
 Thermus thermophilus S-adenosyl-L-homocysteine + yeast tRNA-Phe containing 2'-O-methylguanine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.1.1.34 SpoU
-
 Thermus thermophilus
2.1.1.34 TrmH
-
 Thermus thermophilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.1.1.34 Thermus thermophilus
-
-
 9.63