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Literature summary extracted from

  • Qu, Q.; Lee, S-J.; Boos, W.
    TreT, a novel trehalose glycosyltransferring synthase of the hyperthermophilic archaeon Thermococcus litoralis (2004), J. Biol. Chem., 279, 47890-47897.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.4.1.245 expression in Escherichia coli, with His-tag Thermococcus litoralis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.4.1.245 1.14
-
 ADP-glucose 90°C, pH 6.5 Thermococcus litoralis
2.4.1.245 6.2
-
 D-glucose 90°C, pH 6.5 Thermococcus litoralis
2.4.1.245 11.5
-
 alpha,alpha-1,1-trehalose 90°C, pH 6.5 Thermococcus litoralis

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.4.1.245 Ca2+ 91% of the activity with Mg2+ Thermococcus litoralis
2.4.1.245 Co2+ 72% of the activity with Mg2+ Thermococcus litoralis
2.4.1.245 Mg2+ required Thermococcus litoralis
2.4.1.245 Mn2+ 78% of the activity with Mg2+ Thermococcus litoralis
2.4.1.245 Ni2+ 57% of the activity with Mg2+ Thermococcus litoralis

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.4.1.245 48000
-
 2 * 48146, calculated, 2 * 48000, SDS-PAGE Thermococcus litoralis
2.4.1.245 48146
-
 2 * 48146, calculated, 2 * 48000, SDS-PAGE Thermococcus litoralis
2.4.1.245 104000
-
 PAGE Thermococcus litoralis

Organism

EC Number Organism UniProt Comment Textmining
2.4.1.245 Thermococcus litoralis
-
 induced by trehalose and maltose in growth medium
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.4.1.245 ADP-alpha-D-glucose + D-glucose
-
 Thermococcus litoralis ADP + alpha,alpha-1,1-trehalose
-
 r
2.4.1.245 alpha,alpha-1,1-trehalose + ADP
-
 Thermococcus litoralis ADP-glucose + D-glucose
-
 r
2.4.1.245 GDP-glucose + D-glucose 5% of the efficiency with ADP-glucose Thermococcus litoralis alpha,alpha-1,1-trehalose + GDP
-
 r
2.4.1.245 additional information reaction is reversible, formation of trehalose is favored by rate of reaction and equilibrium. No substrate: glucose 1-phosphate, glucose 6-phosphate, fructose, mannose, galactose, xylose, 2-deoxyglucose, glucosamine, alpha-methylglucoside, sorbitol Thermococcus litoralis ?
-
 ?
2.4.1.245 UDP-glucose + D-glucose 6% of the efficiency with ADP-glucose Thermococcus litoralis alpha,alpha-1,1-trehalose + UDP
-
 r

Subunits

EC Number Subunits Comment Organism
2.4.1.245 dimer 2 * 48146, calculated, 2 * 48000, SDS-PAGE Thermococcus litoralis

Synonyms

EC Number Synonyms Comment Organism
2.4.1.245 TreT
-
 Thermococcus litoralis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.4.1.245 90
-
-
 Thermococcus litoralis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.4.1.245 100
-
 65% residual activity Thermococcus litoralis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.4.1.245 6.5
-
-
 Thermococcus litoralis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.4.1.245 7.5
-
 13% residual activity Thermococcus litoralis