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Literature summary extracted from

  • Jonsson, S.; Ricagno, S.; Lindqvist, Y.; Richards, N.G.
    Kinetic and mechanistic characterization of the formyl-CoA transferase from Oxalobacter formigenes (2004), J. Biol. Chem., 279, 36003-36012.
    View publication on PubMed

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.8.3.16 crystallization and structure determination of the enzyme/oxalyl-CoA complex, hanging drop technique. Crystallization and structure determination of the D169A, D169E, and D169S mutants Oxalobacter formigenes

Protein Variants

EC Number Protein Variants Comment Organism
2.8.3.16 D169A mutant enzyme is correctly folded and forms interlocked dimers, 1300fold decrease in activity Oxalobacter formigenes
2.8.3.16 D169E mutant enzyme is correctly folded and forms interlocked dimers, inactive mutant enzyme Oxalobacter formigenes
2.8.3.16 D169S mutant enzyme is correctly folded and forms interlocked dimers, inactive mutant enzyme Oxalobacter formigenes

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.8.3.16 formate
-
 Oxalobacter formigenes
2.8.3.16 oxalyl-CoA
-
 Oxalobacter formigenes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.8.3.16 81000
-
 gel filtration Oxalobacter formigenes

Organism

EC Number Organism UniProt Comment Textmining
2.8.3.16 Oxalobacter formigenes
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.8.3.16 enzyme recombinantly expressed in Escherichia coli Oxalobacter formigenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.8.3.16 formyl-CoA + oxalate involvement of the Asp169 side chain in mediating enzyme-catalyzed CoA transfer via a series of anhydride intermediates Oxalobacter formigenes formate + oxalyl-CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
2.8.3.16 dimer
-
 Oxalobacter formigenes

Synonyms

EC Number Synonyms Comment Organism
2.8.3.16 FRC
-
 Oxalobacter formigenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.8.3.16 4.3
-
 oxalate
-
 Oxalobacter formigenes
2.8.3.16 4.3
-
 formyl-CoA
-
 Oxalobacter formigenes

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.8.3.16 additional information
-
 additional information Kis for formate is 17 mM, Kii for formate is 380 mM, Kis for oxalyl-CoA is 0.15 mM, Kii for oxalyl-CoA is 0.28 mM Oxalobacter formigenes