Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Lewin, T.M.; Schwerbrock, N.M.; Lee, D.P.; Coleman, R.A.
    Identification of a new glycerol-3-phosphate acyltransferase isoenzyme, mtGPAT2, in mitochondria (2004), J. Biol. Chem., 279, 13488-13495.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.3.1.15 acetone activates isozyme mtGPAT1, no activation of isozyme mtGPAT2 Mus musculus
2.3.1.15 Polymyxin B activates isozyme mtGPAT1 Mus musculus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.3.1.15 elimination of isozyme mtGPAT1 in knockout mice Mus musculus

Protein Variants

EC Number Protein Variants Comment Organism
2.3.1.15 additional information construction of isozyme mtGPAT1-knockout mice Mus musculus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.3.1.15 CaCl2 33% inhibition of isozyme mtGPAT1, 70% inhibition of isozyme mtGPAT2 Mus musculus
2.3.1.15 dihydroxyacetonephosphate competitive inhibition of isozyme mtGPAT2, no inhibition of isozyme mtGPAT1 Mus musculus
2.3.1.15 lysophosphatidic acid product inhibition, all isozymes Mus musculus
2.3.1.15 additional information no inhibition by malonyl-CoA, substrate inhibition by acyl-CoAs at concentrations above 0.075 mM Mus musculus
2.3.1.15 NEM inactivation of isozyme mtGPAT2 and the microsomal isozyme Mus musculus
2.3.1.15 Polymyxin B inhibitis isozyme mtGPAT2 and the microsomal isozyme Mus musculus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.3.1.15 0.14
-
 sn-glycerol 3-phosphate pH 7.5, 22°C, microsomal isozyme Mus musculus
2.3.1.15 0.3
-
 sn-glycerol 3-phosphate pH 7.5, 22°C, isozyme mtGPAT1-knockout-mice mitochondria Mus musculus
2.3.1.15 0.4
-
 sn-glycerol 3-phosphate pH 7.5, 22°C, wild-type mitochondria Mus musculus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.15 endoplasmic reticulum membrane microsomal isozyme Mus musculus 5789
-
2.3.1.15 mitochondrion 2 isozymes in mitochondria, mitochondrial isozymes mtGPAT1 and mtGPAT2 Mus musculus 5739
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.3.1.15 DTT
-
 Mus musculus
2.3.1.15 Mg2+ best at 4 mM MgCl2 Mus musculus
2.3.1.15 NaF activates Mus musculus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate Mus musculus enzyme catalyzes the initial and rate-limiting step of glycerolipid synthesis with long-chain acyl-CoAs CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.15 Mus musculus
-
 3 isozymes
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.15 isozymes partially by preparation of subcellular fractions Mus musculus

Reaction

EC Number Reaction Comment Organism Reaction ID
2.3.1.15 an acyl-CoA + sn-glycerol 3-phosphate = CoA + a 1-acyl-sn-glycerol 3-phosphate the active site is composed of homology regions I-IV Mus musculus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.3.1.15 liver
-
 Mus musculus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 acyl-CoA + sn-glycerol 3-phosphate enzyme catalyzes the initial and rate-limiting step of glycerolipid synthesis with long-chain acyl-CoAs Mus musculus CoA + 1-acyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 additional information the mitochondrial and microsomal isozymes show different substrate specificity Mus musculus ?
-
 ?
2.3.1.15 oleoyl-CoA + sn-glycerol 3-phosphate
-
 Mus musculus CoA + 1-oleoyl-sn-glycerol 3-phosphate
-
 ?
2.3.1.15 palmitoyl-CoA + sn-glycerol 3-phosphate preferred substrate of isozyme mtGPAT1, not of isozyme mtGPAT2 Mus musculus CoA + 1-palmitoyl-sn-glycerol 3-phosphate
-
 ?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.15 glycerol-3-phosphate acyltransferase-1
-
 Mus musculus
2.3.1.15 mtGPAT2
-
 Mus musculus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.3.1.15 22
-
 assay at room temperature Mus musculus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.3.1.15 additional information
-
 isozyme mtGPAT2 is temperature-sensitive Mus musculus
2.3.1.15 40.5
-
 half-life of microsomal isozyme is 7 min, loss of 80% activity of isozyme mtGPAT2 after 4 min Mus musculus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.3.1.15 7 7.5
-
 Mus musculus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.3.1.15 0.545
-
 dihydroxyacetonephosphate pH 7.5, 22°C, isozyme mtGPAT2 Mus musculus