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Literature summary extracted from
- Structural and kinetic studies of sugar binding to galactose mutarotase from Lactococcus lactis (2002), J. Biol. Chem., 277, 45458-45465.
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 5.1.3.3 | in complex with D-glucose, D-fucose, D-quinovose, L-arabinose, or D-xylose | Lactococcus lactis |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.3 | 20 | - |
alpha-D-galactose | pH 7.6, 25°C | Lactococcus lactis |  |
| 5.1.3.3 | 21 | - |
alpha-D-xylose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 25 | - |
alpha-D-Fucose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 32 | - |
alpha-L-arabinose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 34 | - |
alpha-D-glucose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 37 | - |
alpha-D-quinovose | pH 7.6, 25°C | Lactococcus lactis | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.1.3.3 | Lactococcus lactis | - |
recombinant protein expressed in Escherichia coli | - |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.1.3.3 | alpha-D-fucose | - |
Lactococcus lactis | beta-D-fucose | - |
? | |
| 5.1.3.3 | alpha-D-galactose | - |
Lactococcus lactis | beta-D-galactose | - |
? | |
| 5.1.3.3 | alpha-D-glucose | - |
Lactococcus lactis | beta-D-glucose | - |
? | |
| 5.1.3.3 | alpha-D-quinovose | - |
Lactococcus lactis | beta-D-quinovose | - |
? | |
| 5.1.3.3 | alpha-D-xylose | - |
Lactococcus lactis | beta-D-xylose | - |
? | |
| 5.1.3.3 | alpha-L-arabinose | - |
Lactococcus lactis | beta-L-arabinose | - |
? | |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.1.3.3 | 410 | - |
alpha-D-xylose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 430 | - |
alpha-D-glucose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 490 | - |
alpha-D-quinovose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 1700 | - |
alpha-D-Fucose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 3700 | - |
alpha-D-galactose | pH 7.6, 25°C | Lactococcus lactis | |
| 5.1.3.3 | 5500 | - |
alpha-L-arabinose | pH 7.6, 25°C | Lactococcus lactis | |
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