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Literature summary extracted from
- Catalytic properties and inhibition of hepatic cholesterol-epoxide hydrolase (1986), J. Biol. Chem., 261, 54-59.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.3.2.9 | cyclohexane oxide | - |
Rattus norvegicus |  |
| 3.3.2.9 | additional information | no inhibition by isoquinoline, 7-oxocholesterol, 7-oxocholestanol, and 6-oxocholestanol | Rattus norvegicus | |
| 3.3.2.11 | 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | competitive to 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
| 3.3.2.11 | 5,6beta-epoxy-5beta-cholestan-3beta-ol | competitive to 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
| 3.3.2.11 | 6-oxocholestanol | - |
Rattus norvegicus | |
| 3.3.2.11 | 7-oxocholestanol | - |
Rattus norvegicus | |
| 3.3.2.11 | 7-oxocholesterol | - |
Rattus norvegicus | |
| 3.3.2.11 | cholestane-3beta,5alpha,6beta-triol | product inhibition | Rattus norvegicus | |
| 3.3.2.11 | additional information | no inhibition by isoquinoline, styrene oxide, cyclohexane oxide, benzil, cholesterol, and cholic acid | Rattus norvegicus |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.11 | additional information | - |
additional information | kinetics | Rattus norvegicus | |
| 3.3.2.11 | 0.0037 | - |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
| 3.3.2.11 | 0.0044 | - |
5,6beta-epoxy-5beta-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.3.2.9 | microsome | - |
Rattus norvegicus | - |
- |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.11 | 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 3.3.2.11 | 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | Rattus norvegicus | - |
cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 3.3.2.11 | additional information | Rattus norvegicus | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | ? | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.3.2.9 | Rattus norvegicus | - |
male sprague-dawley rats | - |
| 3.3.2.11 | Rattus norvegicus | - |
male sprague-dawley rats | - |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.3.2.11 | partially by microsome preparation | Rattus norvegicus |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|---|
| 3.3.2.9 | liver | - |
Rattus norvegicus | - |
| 3.3.2.11 | liver | - |
Rattus norvegicus | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.11 | 0.00045 | - |
liver microsomes, substrate 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
| 3.3.2.11 | 0.00048 | - |
liver microsomes, substrate 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.3.2.9 | additional information | no activity with 5,6alpha-epoxy-5alpha-cholestan-3beta-ol and 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | ? | - |
? | |
| 3.3.2.9 | styrene epoxide + H2O | - |
Rattus norvegicus | 1-phenyl-ethane-1,2-diol | - |
? | |
| 3.3.2.11 | 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 3.3.2.11 | 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 3.3.2.11 | 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | - |
Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 3.3.2.11 | 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O | the enzyme is equally active with both stereoisomers | Rattus norvegicus | cholestane-3beta,5alpha,6beta-triol | - |
? | |
| 3.3.2.11 | additional information | the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 | Rattus norvegicus | ? | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 3.3.2.9 | styrene-epoxide hydrolase | - |
Rattus norvegicus |
| 3.3.2.9 | xenobiotic epoxide hydrolase | - |
Rattus norvegicus |
| 3.3.2.11 | cholesterol-epoxide hydrolase | - |
Rattus norvegicus |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.9 | 37 | - |
assay at | Rattus norvegicus |
| 3.3.2.11 | 37 | - |
assay at | Rattus norvegicus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.3.2.9 | 7.4 | - |
assay at | Rattus norvegicus |
| 3.3.2.11 | 7.4 | - |
assay at | Rattus norvegicus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.3.2.9 | 0.0154 | - |
cyclohexane oxide | pH 7.4, 37°C | Rattus norvegicus | |
| 3.3.2.11 | 0.0037 | - |
5,6beta-epoxy-5beta-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6alpha-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
| 3.3.2.11 | 0.0044 | - |
5,6alpha-epoxy-5alpha-cholestan-3beta-ol | pH 7.4, 37°C, versus 5,6beta-epoxy-5alpha-cholestan-3beta-ol | Rattus norvegicus | |
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