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Literature summary extracted from

  • Nashed, N.T.; Michaud, D.P.; Levin, W.; Jerina, D.M.
    7-Dehydrocholesterol 5,6 beta-oxide as a mechanism-based inhibitor of microsomal cholesterol oxide hydrolase (1986), J. Biol. Chem., 261, 2510-2513.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.3.2.11 acetonitrile activates at 3% v/v Rattus norvegicus

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.3.2.11 5,6alpha-imino-5alpha-cholestan-3beta-ol strong, specific, competitive inhibition Rattus norvegicus
3.3.2.11 7-dehydrocholesterol 5,6 beta-oxide specific, mechanism-based inhibitor Rattus norvegicus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
3.3.2.11 microsome
-
 Rattus norvegicus
-
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O Rattus norvegicus
-
 cholestane-3beta,5alpha,6beta-triol
-
 ?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O Rattus norvegicus
-
 cholestane-3beta,5alpha,6beta-triol
-
 ?
3.3.2.11 additional information Rattus norvegicus the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 ?
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
3.3.2.11 Rattus norvegicus
-
 male Long Evans rats
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.3.2.11 partially by microsome preparation Rattus norvegicus

Reaction

EC Number Reaction Comment Organism Reaction ID
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O = 5alpha-cholestane-3beta,5alpha,6beta-triol positively charged transition state Rattus norvegicus
a
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O = 5alpha-cholestane-3beta,5alpha,6beta-triol positively charged transition state Rattus norvegicus
a

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.3.2.11 liver
-
 Rattus norvegicus
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.3.2.11 5,6alpha-epoxy-5alpha-cholestan-3beta-ol + H2O
-
 Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
 ?
3.3.2.11 5,6beta-epoxy-5beta-cholestan-3beta-ol + H2O
-
 Rattus norvegicus cholestane-3beta,5alpha,6beta-triol
-
 ?
3.3.2.11 additional information the enzyme is distinct from the microsomal epoxide hydrolase EC 3.3.2.9 Rattus norvegicus ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
3.3.2.11 cholesterol oxide hydrolase
-
 Rattus norvegicus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.3.2.11 37
-
 assay at Rattus norvegicus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.3.2.11 7.4
-
-
 Rattus norvegicus

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
3.3.2.11 additional information
-
 additional information inhibition kinetics at pH 8.0, 37°C, 6.5% v/v acetonitrile Rattus norvegicus