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Literature summary extracted from
- Escherichia coli RNase D. Purification and structural characterization of a putative processing nuclease (1981), J. Biol. Chem., 256, 5627-5632.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.1.13.5 | 5,5'-dithiobis(2-nitrobenzoic acid) | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli |  |
| 3.1.13.5 | HgCl2 | the enzyme is relatively insensitive to sulfhydryl reagents, increased inhibition after addition of dithiothreitol | Escherichia coli | |
| 3.1.13.5 | NEM | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli | |
| 3.1.13.5 | p-hydroxymercuribenzoate | the enzyme is relatively insensitive to sulfhydryl reagents | Escherichia coli | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.5 | 40000 | - |
gel filtration | Escherichia coli |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.1.13.5 | Escherichia coli | - |
- |
- |
Posttranslational Modification
| EC Number | Posttranslational Modification | Comment | Organism |
|---|---|---|---|
| 3.1.13.5 | additional information | the enzyme is devoid of nucleic acid | Escherichia coli |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.1.13.5 | - |
Escherichia coli |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.5 | 5 | - |
- |
Escherichia coli |
Storage Stability
| EC Number | Storage Stability | Organism |
|---|---|---|
| 3.1.13.5 | -20°C, 6 months, enzyme retains 90% of its activity | Escherichia coli |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 3.1.13.5 | monomer | 1 * 40000, SDS-PAGE | Escherichia coli |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.1.13.5 | additional information | - |
the enzyme is sensitive to inactivation by elevated temperatures but can be protected by a variety of RNAs | Escherichia coli |
| 3.1.13.5 | 50 | - |
3-4 min, 50% loss of the activity against phosphodiesterase-treated tRNA | Escherichia coli |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 3.1.13.5 | Escherichia coli | electrofocusing, pH-range 2-11 | - |
6.2 |
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