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Literature summary extracted from

  • Park, H.J.; Cho, H.Y.; Kong, K.H.
    Purification and biochemical properties of glutathione S-transferase from Lactuca sativa (2005), J. Biochem. Mol. Biol., 38, 232-237.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 benastatin A IC50: 0.00041 mM Lactuca sativa
2.5.1.18 Ethacrynic acid 0.00028 mM Lactuca sativa
2.5.1.18 S-(2,4-dinitrophenyl)glutathione IC50: 0.0034 mM Lactuca sativa
2.5.1.18 S-hexyl-GSH IC50: 0.0152 mM Lactuca sativa
2.5.1.18 S-methyl-GSH IC50: 0.0123 mM Lactuca sativa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 23000
-
 2 * 23000, SDS-PAGE Lactuca sativa
2.5.1.18 48000
-
 gel filtration Lactuca sativa

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Lactuca sativa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Lactuca sativa

Source Tissue

EC Number Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.5.1.18 4.03
-
-
 Lactuca sativa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1,2-epoxy-3-(p-nitrophenoxy)propane + glutathione
-
 Lactuca sativa ?
-
 ?
2.5.1.18 1-chloro-2,3-dinitrobenzene + glutathione
-
 Lactuca sativa S-(2,3-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 ethacrynic acid + glutathione
-
 Lactuca sativa ?
-
 ?
2.5.1.18 additional information the enzyme also exhibits glutathione peroxidase activity towards cumene hydroperoxide Lactuca sativa ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 2 * 23000, SDS-PAGE Lactuca sativa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.5.1.18 45
-
 10 min, stable up to Lactuca sativa
2.5.1.18 50
-
 10 min, about 40% loss of activity Lactuca sativa
2.5.1.18 60
-
 10 min, about 75% loss of activity Lactuca sativa
2.5.1.18 65
-
 10 min, about 90% loss of activity Lactuca sativa

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00041
-
 IC50: 0.00041 mM Lactuca sativa benastatin A
2.5.1.18 0.0034
-
 IC50: 0.0034 mM Lactuca sativa S-(2,4-dinitrophenyl)glutathione
2.5.1.18 0.0123
-
 IC50: 0.0123 mM Lactuca sativa S-methyl-GSH
2.5.1.18 0.0152
-
 IC50: 0.0152 mM Lactuca sativa S-hexyl-GSH