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Literature summary extracted from
- Purification and characterization of recombinant human prostacyclin synthase (2004), J. Biochem., 135, 455-463.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.3.99.4 | expression in Spodoptera frugiperda 21 cells | Homo sapiens |
Protein Variants
| EC Number | Protein Variants | Comment | Organism |
|---|---|---|---|
| 5.3.99.4 | C441H | no heme-binding ability, no activity | Homo sapiens |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.4 | tranylcypromine | - |
Homo sapiens |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.3.99.4 | 0.03 | - |
prostaglandin H2 | at 24°C | Homo sapiens | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.3.99.4 | microsome | localization of recombinant enzyme in infected Sf21 cells | Homo sapiens | - |
- |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.4 | 48000 | - |
wild-type, SDS-PAGE | Homo sapiens |
| 5.3.99.4 | 48000 | - |
mutant C441H, SDS-PAGE | Homo sapiens |
| 5.3.99.4 | 57100 | - |
calculated from the primary structure, deduced from the cDNA sequence | Homo sapiens |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.4 | prostaglandin H2 | Homo sapiens | - |
prostacyclin | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.3.99.4 | Homo sapiens | - |
- |
- |
Oxidation Stability
| EC Number | Oxidation Stability | Organism |
|---|---|---|
| 5.3.99.4 | human PGIS is significantly unstable when the heme cofactor appears in the reduction and/or reduction-CO binding state | Homo sapiens |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.3.99.4 | recombinant enzyme is purified to homogeneity | Homo sapiens |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 5.3.99.4 | 1.6 | - |
solubilized microsomes | Homo sapiens |
| 5.3.99.4 | 2.6 | - |
after calcium phosphate gel | Homo sapiens |
| 5.3.99.4 | 4.3 | - |
after DEAE-sepharose chromatography | Homo sapiens |
| 5.3.99.4 | 7.1 | - |
after hydroxyapatite chromatography | Homo sapiens |
| 5.3.99.4 | 15 | - |
purified enzyme, at 24°C | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.3.99.4 | prostaglandin H2 | - |
Homo sapiens | prostacyclin | - |
? | |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.3.99.4 | PGIS | - |
Homo sapiens |
| 5.3.99.4 | prostacyclin synthase | - |
Homo sapiens |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.3.99.4 | heme | - |
Homo sapiens | |
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