BRENDA - Enzyme Database show

Purification and characterization of recombinant human prostacyclin synthase

Wada, M.; Yokoyama, C.; Hatae, T.; Shimonishi, M.; Nakamura, M.; Imai, Y.; Ullrich, V.; Tanabe, T.; J. Biochem. 135, 455-463 (2004)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
5.3.99.4
expression in Spodoptera frugiperda 21 cells
Homo sapiens
Engineering
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.4
C441H
no heme-binding ability, no activity
Homo sapiens
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.4
Tranylcypromine
-
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.4
0.03
-
prostaglandin H2
at 24°C
Homo sapiens
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
microsome
localization of recombinant enzyme in infected Sf21 cells
Homo sapiens
-
-
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.4
48000
-
mutant C441H, SDS-PAGE; wild-type, SDS-PAGE
Homo sapiens
5.3.99.4
57100
-
calculated from the primary structure, deduced from the cDNA sequence
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
prostaglandin H2
Homo sapiens
-
prostacyclin
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
5.3.99.4
Homo sapiens
-
-
-
Oxidation Stability
EC Number
Oxidation Stability
Organism
5.3.99.4
human PGIS is significantly unstable when the heme cofactor appears in the reduction and/or reduction-CO binding state
Homo sapiens
Purification (Commentary)
EC Number
Commentary
Organism
5.3.99.4
recombinant enzyme is purified to homogeneity
Homo sapiens
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.4
1.6
-
solubilized microsomes
Homo sapiens
5.3.99.4
2.6
-
after calcium phosphate gel
Homo sapiens
5.3.99.4
4.3
-
after DEAE-sepharose chromatography
Homo sapiens
5.3.99.4
7.1
-
after hydroxyapatite chromatography
Homo sapiens
5.3.99.4
15
-
purified enzyme, at 24°C
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
prostaglandin H2
-
662059
Homo sapiens
prostacyclin
-
-
-
?
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.4
heme
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.4
expression in Spodoptera frugiperda 21 cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
5.3.99.4
heme
-
Homo sapiens
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
5.3.99.4
C441H
no heme-binding ability, no activity
Homo sapiens
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
5.3.99.4
Tranylcypromine
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
5.3.99.4
0.03
-
prostaglandin H2
at 24°C
Homo sapiens
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
5.3.99.4
microsome
localization of recombinant enzyme in infected Sf21 cells
Homo sapiens
-
-
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
5.3.99.4
48000
-
mutant C441H, SDS-PAGE; wild-type, SDS-PAGE
Homo sapiens
5.3.99.4
57100
-
calculated from the primary structure, deduced from the cDNA sequence
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
5.3.99.4
prostaglandin H2
Homo sapiens
-
prostacyclin
-
-
?
Oxidation Stability (protein specific)
EC Number
Oxidation Stability
Organism
5.3.99.4
human PGIS is significantly unstable when the heme cofactor appears in the reduction and/or reduction-CO binding state
Homo sapiens
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
5.3.99.4
recombinant enzyme is purified to homogeneity
Homo sapiens
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
5.3.99.4
1.6
-
solubilized microsomes
Homo sapiens
5.3.99.4
2.6
-
after calcium phosphate gel
Homo sapiens
5.3.99.4
4.3
-
after DEAE-sepharose chromatography
Homo sapiens
5.3.99.4
7.1
-
after hydroxyapatite chromatography
Homo sapiens
5.3.99.4
15
-
purified enzyme, at 24°C
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
5.3.99.4
prostaglandin H2
-
662059
Homo sapiens
prostacyclin
-
-
-
?