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Literature summary extracted from

  • Hosono, A.; Mizuguchi, H.; Hayashi, H.; Goto, M.; Miyahara, I.; Hirotsu, K.; Kagamiyama, H.
    Glutamine:phenylpyruvate aminotransferase from an extremely thermophilic bacterium, Thermus thermophilus HB8 (2003), J. Biochem., 134, 843-851.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.64
-
 Thermus thermophilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.64 0.06
-
 pyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.13
-
 phenylpyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.14
-
 4-hydroxyphenylpyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.16
-
 Indolepyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.76
-
 L-phenylalanine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.82
-
 L-tyrosine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 1
-
 L-asparagine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 3.2
-
 L-methionine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 3.2
-
 L-kynurenine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 3.5
-
 L-glutamine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 6
-
 L-tryptophan pH 8.0, 25°C Thermus thermophilus
2.6.1.64 18
-
 L-histidine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 19
-
 L-aspartate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 21
-
 L-glutamate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 25
-
 2-oxoglutarate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 37
-
 L-serine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 39
-
 2-oxoglutarate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 55
-
 L-threonine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 63
-
 L-alanine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 130
-
 L-arginine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 180
-
 L-leucine pH 8.0, 25°C Thermus thermophilus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.6.1.64 42000
-
 2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence Thermus thermophilus
2.6.1.64 42271
-
 2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence Thermus thermophilus
2.6.1.64 82000
-
 gel filtration Thermus thermophilus

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.64 Thermus thermophilus
-
 expression in Escherichia coli
-
2.6.1.64 Thermus thermophilus HB8 / ATCC 27634 / DSM 579
-
 expression in Escherichia coli
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.64 L-arginine + pyruvate
-
 Thermus thermophilus 5-guanidino-2-oxopentanoate + L-alanine
-
 r
2.6.1.64 L-asparagine + phenylpyruvate
-
 Thermus thermophilus 2-oxosuccinamate + L-phenylalanine
-
 r
2.6.1.64 L-aspartate + pyruvate
-
 Thermus thermophilus oxaloacetate + L-alanine
-
 r
2.6.1.64 L-aspartate + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 oxaloacetate + L-alanine
-
 r
2.6.1.64 L-glutamate + phenylpyruvate
-
 Thermus thermophilus 2-oxoglutarate + L-phenylalanine
-
 r
2.6.1.64 L-glutamine + pyruvate
-
 Thermus thermophilus 2-oxoglutaramate + L-alanine
-
 ?
2.6.1.64 L-histidine + pyruvate
-
 Thermus thermophilus 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ?
2.6.1.64 L-histidine + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 3-(1H-imidazol-4-yl)-2-oxopropanoate + L-alanine
-
 ?
2.6.1.64 L-kynurenine + pyruvate
-
 Thermus thermophilus 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 r
2.6.1.64 L-kynurenine + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 4-(2-aminophenyl)-2,4-dioxobutanoate + L-alanine
-
 r
2.6.1.64 L-leucine + phenylpyruvate
-
 Thermus thermophilus 4-methyl-2-oxopentanoate + L-phenylalanine
-
 r
2.6.1.64 L-methionine + phenylpyruvate
-
 Thermus thermophilus 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
2.6.1.64 L-methionine + phenylpyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 4-methylsulfanyl-2-oxobutanoate + L-phenylalanine
-
 r
2.6.1.64 L-phenylalanine + 2-oxoglutarate
-
 Thermus thermophilus ?
-
 ?
2.6.1.64 L-phenylalanine + 4-hydroxyphenylpyruvate
-
 Thermus thermophilus phenylpyruvate + L-tyrosine
-
 r
2.6.1.64 L-phenylalanine + 4-methylsulfanyl-2-oxobutanoate
-
 Thermus thermophilus phenylpyruvate + L-methionine
-
 r
2.6.1.64 L-phenylalanine + indolepyruvate
-
 Thermus thermophilus phenylpyruvate + L-tryptophan
-
 r
2.6.1.64 L-phenylalanine + pyruvate
-
 Thermus thermophilus phenylpyruvate + L-alanine
-
 r
2.6.1.64 L-phenylalanine + pyruvate
-
 Thermus thermophilus HB8 / ATCC 27634 / DSM 579 phenylpyruvate + L-alanine
-
 r
2.6.1.64 L-serine + pyruvate
-
 Thermus thermophilus 3-hydroxy-2-oxopropanoate + L-alanine
-
 r
2.6.1.64 L-threonine + pyruvate
-
 Thermus thermophilus 3-hydroxy-2-oxobutanoate + L-alanine
-
 r
2.6.1.64 L-tryptophan + pyruvate
-
 Thermus thermophilus indolepyruvate + L-alanine
-
 r
2.6.1.64 L-tyrosine + 2-oxoglutaramate
-
 Thermus thermophilus 3-(4-hydroxyphenyl)-2-oxopropanoate + L-glutamine
-
 r
2.6.1.64 phenylpyruvate + L-alanine
-
 Thermus thermophilus L-phenylalanine + pyruvate
-
 r

Subunits

EC Number Subunits Comment Organism
2.6.1.64 dimer 2 * 42271, calculated, 2 * 42000, SDS-PAGE, N-terminal amino acid sequence Thermus thermophilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.6.1.64 0.013
-
 L-threonine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.016
-
 L-aspartate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.03
-
 L-glutamate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.12
-
 L-arginine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.13
-
 L-asparagine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 0.18
-
 L-alanine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 1.2
-
 L-serine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 1.3
-
 L-histidine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 1.5
-
 L-leucine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 3.1
-
 2-oxoglutarate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 15
-
 pyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 130
-
 phenylpyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 480
-
 Indolepyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 590
-
 L-phenylalanine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 620
-
 4-hydroxyphenylpyruvate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 620
-
 4-methylsulfanyl-2-oxobutanoate pH 8.0, 25°C Thermus thermophilus
2.6.1.64 830
-
 L-glutamine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 970
-
 L-tyrosine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 990
-
 L-tryptophan pH 8.0, 25°C Thermus thermophilus
2.6.1.64 1100
-
 L-kynurenine pH 8.0, 25°C Thermus thermophilus
2.6.1.64 1200
-
 L-methionine pH 8.0, 25°C Thermus thermophilus