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Literature summary extracted from
- Copurification and characterization of deacetoxycephalosporin C synthetase/hydroxylase from Cephalosporium acremonium (1987), J. Bacteriol., 169, 1611-1618.
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.26 | 5,5'-dithiobis-2-nitrobenzoic acid | 1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum |  |
| 1.14.11.26 | Ca2+ | weak | Acremonium chrysogenum | |
| 1.14.11.26 | EDTA | 0.6 mM, 83% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
| 1.14.11.26 | iodoacetic acid | 1 mM, 2% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
| 1.14.11.26 | N-ethylmaleimide | 1 mM, 97% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
| 1.14.11.26 | o-phenanthroline | 0.6 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
| 1.14.11.26 | p-hydroxymercuribenzoate | 1 mM, 100% inhibition of hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum | |
| 1.14.11.26 | Zn2+ | strong | Acremonium chrysogenum | |
| 1.14.20.1 | 5,5'-dithiobis-2-nitrobenzoic acid | 1 mM, 100% inhibition of expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum | |
| 1.14.20.1 | Ca2+ | weak | Acremonium chrysogenum | |
| 1.14.20.1 | EDTA | 0.6 mM, 89% inhibition of expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum | |
| 1.14.20.1 | iodoacetic acid | 1 mM, 4% inhibition of expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum | |
| 1.14.20.1 | N-ethylmaleimide | 1 mM, 51% inhibition of expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum | |
| 1.14.20.1 | o-phenanthroline | 0.6 mM, 100% inhibition of expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum | |
| 1.14.20.1 | p-hydroxymercuribenzoate | 1 mM, 100% inhibition of expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum | |
| 1.14.20.1 | Zn2+ | strong | Acremonium chrysogenum | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.14.11.26 | 0.02 | - |
deacetoxycephalosporin C | pH 7.5, 36°C | Acremonium chrysogenum | |
| 1.14.11.26 | 0.022 | - |
2-oxoglutarate | pH 7.5, 36°C | Acremonium chrysogenum | |
| 1.14.20.1 | 0.022 | - |
2-oxoglutarate | pH 7.5, 30°C | Acremonium chrysogenum | |
| 1.14.20.1 | 0.029 | - |
Penicillin N | pH 7.5, 30°C | Acremonium chrysogenum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.14.11.26 | Fe2+ | required for both activities | Acremonium chrysogenum | |
| 1.14.11.26 | additional information | Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ | Acremonium chrysogenum | |
| 1.14.20.1 | Fe2+ | required for both activities | Acremonium chrysogenum | |
| 1.14.20.1 | additional information | Fe2+ cannot be replaced by Mg2+, Mn2+, Ca2+, Co2+, Cu2+, Ni2+ or Zn2+ | Acremonium chrysogenum |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.26 | 41000 | - |
1 * 41000, SDS-PAGE | Acremonium chrysogenum |
| 1.14.11.26 | 43000 | - |
gel filtration | Acremonium chrysogenum |
| 1.14.20.1 | 41000 | - |
1 * 41000, SDS-PAGE | Acremonium chrysogenum |
| 1.14.20.1 | 43000 | - |
gel filtration | Acremonium chrysogenum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.14.11.26 | Acremonium chrysogenum | - |
catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
| 1.14.20.1 | Acremonium chrysogenum | - |
catalyzes both reaction of EC 1.14.20.1 and of EC 1.14.11.26 | - |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.26 | 0.127 | - |
pH 7.5, 36°C | Acremonium chrysogenum |
| 1.14.20.1 | 0.827 | - |
pH 7.5, 30°C | Acremonium chrysogenum |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.14.11.26 | deacetoxycephalosporin C + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetylcephalosporin C + succinate + CO2 | - |
? | |
| 1.14.20.1 | penicillin N + 2-oxoglutarate + O2 | - |
Acremonium chrysogenum | deacetoxycephalosporin C + succinate + CO2 + H2O | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.14.11.26 | monomer | 1 * 41000, SDS-PAGE | Acremonium chrysogenum |
| 1.14.20.1 | monomer | 1 * 41000, SDS-PAGE | Acremonium chrysogenum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.26 | 36 | 38 | - |
Acremonium chrysogenum |
| 1.14.20.1 | 26 | 34 | expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.14.11.26 | 7.3 | - |
hydroxylation reaction, EC 1.14.11.26 | Acremonium chrysogenum |
| 1.14.20.1 | 7.5 | 7.8 | expandase reaction, EC 1.14.20.1 | Acremonium chrysogenum |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.14.11.26 | Acremonium chrysogenum | isoelectric focusing | - |
6.3 |
| 1.14.20.1 | Acremonium chrysogenum | isoelectric focusing | - |
6.3 |
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