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Literature summary extracted from

  • Lucchesi, G.I.; Domenech, C.E.
    A simple and reliable method for the purification of Pseudomonas aeruginosa phospholipase C produced in a high phosphate medium containing choline (1994), Int. J. Biochem., 26, 155-162.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.1.4.3 Triton X-100 30% inhibition by 1% Triton X-100 Pseudomonas aeruginosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.1.4.3 25
-
 p-Nitrophenylphosphorylcholine 37°C, pH 7.4 Pseudomonas aeruginosa

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.1.4.3 70000
-
 x * 70000, SDS-PAGE Pseudomonas aeruginosa

Organism

EC Number Organism UniProt Comment Textmining
3.1.4.3 Pseudomonas aeruginosa
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
3.1.4.3
-
 Pseudomonas aeruginosa

Source Tissue

EC Number Source Tissue Comment Organism Textmining
3.1.4.3 culture supernatant
-
 Pseudomonas aeruginosa
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.1.4.3 13.27
-
-
 Pseudomonas aeruginosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.1.4.3 p-nitrophenylphosphorylcholine + H2O
-
 Pseudomonas aeruginosa p-nitrophenol + phosphorylcholine
-
 ?

Subunits

EC Number Subunits Comment Organism
3.1.4.3 ? x * 70000, SDS-PAGE Pseudomonas aeruginosa

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.1.4.3 100
-
 1 min, activity is destroyed Pseudomonas aeruginosa