EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.136 | expression of wild-type and mutant enzymes in strains TG1 and N43 | Escherichia coli |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.136 | H198A | site-directed mutagenesis, 50% reduction of the minimal inhibitory concentration MIC level of oleandomycin and 50% reduced activity compared to the wild-type enzyme | Escherichia coli |
2.7.1.136 | H205A | site-directed mutagenesis, 87.5% reduction of the minimal inhibitory concentration MIC level of oleandomycin and 50% reduced activity compared to the wild-type enzyme | Escherichia coli |
2.7.1.136 | H205N | site-directed mutagenesis, unaffected minimal inhibitory concentration MIC level of oleandomycin, but more than 99% reduced activity compared to the wild-type enzyme | Escherichia coli |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.136 | oleandomycin | - |
Escherichia coli |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.136 | KCl | - |
Escherichia coli | |
2.7.1.136 | Mg2+ | - |
Escherichia coli |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.136 | Escherichia coli | - |
gene mphB | - |
2.7.1.136 | Escherichia coli CU1 | - |
gene mphB | - |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.136 | recombinant wild-type and mutant enzymes from strain TG1 | Escherichia coli |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.136 | additional information | - |
substrate specificities of the mutant enzymes | Escherichia coli |
2.7.1.136 | 0.0007 | - |
purified recombinant mutant H205A, substrate oleandomycin | Escherichia coli |
2.7.1.136 | 0.04 | - |
purified recombinant wild-type enzyme, substrate roxithromycin or rokitamycin | Escherichia coli |
2.7.1.136 | 0.05 | - |
purified recombinant wild-type enzyme, substrate josamycin or tylosin | Escherichia coli |
2.7.1.136 | 0.052 | - |
purified recombinant mutant H198A, substrate oleandomycin | Escherichia coli |
2.7.1.136 | 0.056 | - |
purified recombinant mutant H205N, substrate oleandomycin | Escherichia coli |
2.7.1.136 | 0.06 | - |
purified recombinant wild-type enzyme, substrate troleandomycin, clarithromycin, azithromycin, or erythromycin | Escherichia coli |
2.7.1.136 | 0.1 | - |
purified recombinant wild-type enzyme, substrate oleandomycin | Escherichia coli |
2.7.1.136 | 0.16 | - |
purified recombinant wild-type enzyme, substrate kitasamycin | Escherichia coli |
2.7.1.136 | 0.21 | - |
purified recombinant wild-type enzyme, substrate spiramycin | Escherichia coli |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.136 | ATP + azithromycin | 15-membered ring macrolide | Escherichia coli | ADP + azithromycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + azithromycin | 15-membered ring macrolide | Escherichia coli CU1 | ADP + azithromycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + clarithromycin | 14-membered ring macrolide | Escherichia coli | ADP + clarithromycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + clarithromycin | 14-membered ring macrolide | Escherichia coli CU1 | ADP + clarithromycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + erythromycin | 14-membered ring macrolide | Escherichia coli | ADP + erythromycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + josamycin | 15-membered ring macrolide | Escherichia coli | ADP + josamycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + kitasamycin | 15-membered ring macrolide | Escherichia coli | ADP + kitasamycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + oleandomycin | 14-membered ring macrolide | Escherichia coli | ADP + oleandomycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + oleandomycin | 14-membered ring macrolide | Escherichia coli CU1 | ADP + oleandomycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + rokitamycin | 15-membered ring macrolide | Escherichia coli | ADP + rokitamycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + roxithromycin | 14-membered ring macrolide | Escherichia coli | ADP + roxithromycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + spiramycin | 15-membered ring macrolide | Escherichia coli | ADP + spiramycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + spiramycin | 15-membered ring macrolide | Escherichia coli CU1 | ADP + spiramycin 2'-O-phosphate | - |
? | |
2.7.1.136 | ATP + troleandomycin + H2O | 14-membered ring macrolide | Escherichia coli | ADP + troleandomycin 2'-O-phosphate + acetate | - |
? | |
2.7.1.136 | ATP + tylosin | 15-membered ring macrolide | Escherichia coli | ADP + tylosin 2'-O-phosphate | - |
? | |
2.7.1.136 | additional information | substrate specificities of wild-type and mutant enzymes, structure-function considerations | Escherichia coli | ? | - |
? | |
2.7.1.136 | additional information | substrate specificities of wild-type and mutant enzymes, structure-function considerations | Escherichia coli CU1 | ? | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.136 | macrolide 2'-phosphotransferase II | - |
Escherichia coli |
2.7.1.136 | MPH(2') | - |
Escherichia coli |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
2.7.1.136 | 37 | - |
assay at | Escherichia coli |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
2.7.1.136 | 7.8 | - |
assay at | Escherichia coli |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.136 | ATP | - |
Escherichia coli |