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Literature summary extracted from

  • Willemös, M.; Molgaard, A.; Johansson, E.; Martinussen, J.
    Lid L11 of the glutamine amidotransferase domain of CTP synthase mediates allosteric GTP activation of glutaminase activity (2005), FEBS J., 272, 856-864.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
6.3.4.2 GTP allosteric activator Lactococcus lactis

Protein Variants

EC Number Protein Variants Comment Organism
6.3.4.2 E362Q turnover number for NH4Cl-dependent GTP synthesis reaction is 1.6fold higher than wild-type value Lactococcus lactis
6.3.4.2 G360A 5fold increase in GTP-dependent activation of uncoupled glutamine hydrolysis compared to wild-type enzyme. Turnover number for NH4Cl-dependent GTP synthesis reaction is 1.2fold higher than wild-type value Lactococcus lactis
6.3.4.2 G360P mutant enzyme shows no GTP activation of the uncoupled glutaminase reaction, about 4fold lower turnover number for NH4Cl-dependent CTP synthesis reaction than wild-type enzyme Lactococcus lactis
6.3.4.2 R359M mutant enzyme shows no GTP activation of the uncoupled glutaminase reaction. Turnover number for NH4Cl-dependent GTP synthesis reaction is 1.1fold higher than wild-type value Lactococcus lactis
6.3.4.2 R359P mutant enzyme shows no GTP activation of the uncoupled glutaminase reaction. Turnover number for NH4Cl-dependent GTP synthesis reaction is 1.2fold lower than wild-type value Lactococcus lactis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
6.3.4.2 54
-
NH4+ 30°C, pH 8.0, wild-type enzyme Lactococcus lactis
6.3.4.2 56
-
NH4+ 30°C, pH 8.0, mutant enzyme G360A Lactococcus lactis
6.3.4.2 57
-
NH4+ 30°C, pH 8.0, mutant enzyme R359M Lactococcus lactis
6.3.4.2 78
-
NH4+ 30°C, pH 8.0, mutant enzyme E362Q Lactococcus lactis
6.3.4.2 92
-
NH4+ 30°C, pH 8.0, mutant enzyme G360P Lactococcus lactis
6.3.4.2 92
-
NH4+ 30°C, pH 8.0, mutant enzyme R359P Lactococcus lactis

Organism

EC Number Organism UniProt Comment Textmining
6.3.4.2 Lactococcus lactis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
6.3.4.2
-
Lactococcus lactis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
6.3.4.2 ATP + UTP + NH4+
-
Lactococcus lactis ADP + phosphate + CTP
-
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Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
6.3.4.2 1.8
-
NH4+ 30°C, pH 8.0, mutant enzyme G360P Lactococcus lactis
6.3.4.2 5.1
-
NH4+ 30°C, pH 8.0, mutant enzyme R359P Lactococcus lactis
6.3.4.2 6.3
-
NH4+ 30°C, pH 8.0, wild-type enzyme Lactococcus lactis
6.3.4.2 6.9
-
NH4+ 30°C, pH 8.0, mutant enzyme R359M Lactococcus lactis
6.3.4.2 7.5
-
NH4+ 30°C, pH 8.0, mutant enzyme G360A Lactococcus lactis
6.3.4.2 10.1
-
NH4+ 30°C, pH 8.0, mutant enzyme E362Q Lactococcus lactis