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Literature summary extracted from
- Characterization of the secreted chorismate mutase from the pathogen Mycobacterium tuberculosis (2005), FEBS J., 272, 375-389.
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | expression in Escherichia coli | Mycobacterium tuberculosis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 5.4.99.5 | 8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid | competitive inhibition | Mycobacterium tuberculosis |  |
| 5.4.99.5 | adamantane-1-phosphonate | no inhibitory effect up to concentrations of 0.1 and 1 mM | Mycobacterium tuberculosis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.15 | - |
chorismate | leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
| 5.4.99.5 | 0.18 | - |
chorismate | untagged enzyme, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 5.4.99.5 | cytoplasm | leaderless protein | Mycobacterium tuberculosis | 5737 | - |
| 5.4.99.5 | periplasmic space | full-length protein | Mycobacterium tuberculosis | 42597 | - |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | 18600 | - |
monomer, calculated, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
| 5.4.99.5 | 18600 | - |
monomer, mass spectrometry, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
| 5.4.99.5 | 19540 | - |
monomer, calculated from the variant that lacks 33 N-terminal residues | Mycobacterium tuberculosis |
| 5.4.99.5 | 19540 | - |
monomer, mass spectrometry | Mycobacterium tuberculosis |
| 5.4.99.5 | 19670 | - |
monomer, calculated from the leaderless variant with additional start methionine | Mycobacterium tuberculosis |
| 5.4.99.5 | 19670 | - |
monomer, mass, spectrometry, leaderless variant with additional start methionine | Mycobacterium tuberculosis |
| 5.4.99.5 | 23000 | - |
monomer, SDS-PAGE, 167-residue untagged lederless MtCM | Mycobacterium tuberculosis |
| 5.4.99.5 | 35400 | - |
ultracentrifugation | Mycobacterium tuberculosis |
| 5.4.99.5 | 43400 | - |
gel filtration | Mycobacterium tuberculosis |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | Mycobacterium tuberculosis | - |
Prephenate | - |
? | |
| 5.4.99.5 | Chorismate | Mycobacterium tuberculosis H37Rv | - |
Prephenate | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 5.4.99.5 | Mycobacterium tuberculosis | - |
- |
- |
| 5.4.99.5 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | - |
Mycobacterium tuberculosis |
Renatured (Commentary)
| EC Number | Renatured (Comment) | Organism |
|---|---|---|
| 5.4.99.5 | thermal denaturation shows a sharp transition from the native to the denatured state, with a calculated Tm of 48°C. Upon subsequent cooling, the protein renatured easily | Mycobacterium tuberculosis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 5.4.99.5 | Chorismate | - |
Mycobacterium tuberculosis | Prephenate | - |
? | |
| 5.4.99.5 | Chorismate | - |
Mycobacterium tuberculosis H37Rv | Prephenate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | dimer | alpha2, ultracentrifugation, gel filtration | Mycobacterium tuberculosis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 5.4.99.5 | chorismate mutase | - |
Mycobacterium tuberculosis |
| 5.4.99.5 | CM | - |
Mycobacterium tuberculosis |
| 5.4.99.5 | MtCM | - |
Mycobacterium tuberculosis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 5.4.99.5 | additional information | - |
calculated Tm: 48°C | Mycobacterium tuberculosis |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 50 | - |
chorismate | untagged enzyme, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
| 5.4.99.5 | 56 | - |
chorismate | leaderless MtCM with C-terminal His tag, at 30°C and pH 7.5 | Mycobacterium tuberculosis | |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 5.4.99.5 | 0.0037 | - |
8-hydroxy-2-oxa-bicyclo[3.3.1]non-6-ene-3,5-dicarboxylic acid | - |
Mycobacterium tuberculosis | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 5.4.99.5 | Mycobacterium tuberculosis | calculated | - |
4.9 |
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