Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary extracted from

  • Willmore, W.G.; Storey, K.B.
    Purification and properties of the glutathione S-transferases from the anoxia-tolerant turtle,Trachemys scripta elegans (2005), FEBS J., 272, 3602-3614.
    View publication on PubMed

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.5.1.18 Cibacron blue IC50: 0.048 mM for homodimeric enzyme, IC50: 0.0084 mM for heterodimeric enzyme Trachemys scripta elegans
2.5.1.18 GSSG IC50: 2.0 mM for homodimeric enzyme, IC50: 2.6 mM for heterodimeric enzyme Trachemys scripta elegans
2.5.1.18 iodoacetamide IC50: 40 mM for homodimeric enzyme, 8.7 mM for heterodimeric enzyme Trachemys scripta elegans
2.5.1.18 KCl IC50: 0.33 M for homodimeric enzyme, IC50: 0.18 M for heterodimeric enzyme Trachemys scripta elegans
2.5.1.18 NaCl IC50: 0.332 M for homodimeric enzyme, IC50: 0.17 M for heterodimeric enzyme Trachemys scripta elegans
2.5.1.18 NH4Cl IC50: 0.2 M for homodimeric enzyme, IC50: 0.12 M for heterodimeric enzyme Trachemys scripta elegans
2.5.1.18 Rose bengal IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00047 mM for heterodimeric enzyme Trachemys scripta elegans
2.5.1.18 S-hexylglutathione IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00039 mM for heterodimeric enzyme Trachemys scripta elegans

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.5.1.18 0.38
-
 GSH homodimeric enzyme Trachemys scripta elegans
2.5.1.18 0.6
-
 GSH heterodimeric enzyme Trachemys scripta elegans

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.5.1.18 32600
-
 1 * 32600 + 1 * 36800, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans
2.5.1.18 34000
-
 2 * 34000, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans
2.5.1.18 36800
-
 1 * 32600 + 1 * 36800, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans

Organism

EC Number Organism UniProt Comment Textmining
2.5.1.18 Trachemys scripta elegans
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.5.1.18
-
 Trachemys scripta elegans

Source Tissue

EC Number Source Tissue Comment Organism Textmining
2.5.1.18 liver
-
 Trachemys scripta elegans
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.5.1.18 1-chloro-2,4-dinitrobenzene + GSH
-
 Trachemys scripta elegans S-(2,4-dinitrophenyl)glutathione + HCl
-
 ?
2.5.1.18 cumene hydroperoxide + GSH
-
 Trachemys scripta elegans ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.5.1.18 dimer 1 * 32600 + 1 * 36800, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans
2.5.1.18 dimer 2 * 34000, enzyme occurs as homodimeric form and as heterodimeric form, SDS-PAGE Trachemys scripta elegans

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.5.1.18 7.2
-
-
 Trachemys scripta elegans

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.5.1.18 6.3 7.4 pH 6.3: about 70% of maximal activity, pH 7.4: about 40% of maximal activity Trachemys scripta elegans

pI Value

EC Number Organism Comment pI Value Maximum pI Value
2.5.1.18 Trachemys scripta elegans
-
-
 additional information

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
2.5.1.18 0.00039
-
 IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00039 mM for heterodimeric enzyme Trachemys scripta elegans S-hexylglutathione
2.5.1.18 0.00047
-
 IC50: 0.00031 mM for homodimeric enzyme, IC50: 0.00047 mM for heterodimeric enzyme Trachemys scripta elegans Rose bengal
2.5.1.18 0.0084
-
 IC50: 0.048 mM for homodimeric enzyme, IC50: 0.0084 mM for heterodimeric enzyme Trachemys scripta elegans Cibacron blue
2.5.1.18 2.6
-
 IC50: 2.0 mM for homodimeric enzyme, IC50: 2.6 mM for heterodimeric enzyme Trachemys scripta elegans GSSG
2.5.1.18 40
-
 IC50: 40 mM for homodimeric enzyme, 8.7 mM for heterodimeric enzyme Trachemys scripta elegans iodoacetamide
2.5.1.18 120
-
 IC50: 0.12 M for heterodimeric enzyme Trachemys scripta elegans NH4Cl
2.5.1.18 170
-
 IC50: 0.17 M for heterodimeric enzyme Trachemys scripta elegans NaCl
2.5.1.18 180
-
 IC50: 0.18 M for heterodimeric enzyme Trachemys scripta elegans KCl
2.5.1.18 200
-
 IC50: 0.2 M for homodimeric enzyme Trachemys scripta elegans NH4Cl
2.5.1.18 330
-
 IC50: 0.33 M for homodimeric enzyme Trachemys scripta elegans KCl
2.5.1.18 332
-
 IC50: 0.332 M for homodimeric enzyme Trachemys scripta elegans NaCl