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Literature summary extracted from

  • Hansen, T.; Schonheit, P.
    ADP-dependent 6-phosphofructokinase, an extremely thermophilic, non-allosteric enzyme from the hyperthermophilic, sulfate-reducing archaeon Archaeoglobus fulgidus strain 7324 (2004), Extremophiles, 8, 29-35.
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
2.7.1.146 additional information no allosterical regulation by ADP, AMP, phosphoenolpyruvate, or citrate Archaeoglobus fulgidus

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.7.1.146 additional information no allosterical regulation by ADP, AMP, phosphoenolpyruvate, or citrate Archaeoglobus fulgidus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.1.146 0.3
-
 ADP pH 6.6, 50°C Archaeoglobus fulgidus
2.7.1.146 0.6
-
 ADP pH 6.6, 80°C Archaeoglobus fulgidus
2.7.1.146 1.4
-
 AMP pH 6.6, 80°C Archaeoglobus fulgidus
2.7.1.146 2.2
-
 D-fructose 6-phosphate pH 6.6, 80°C Archaeoglobus fulgidus
2.7.1.146 4.7
-
 D-fructose 6-phosphate pH 6.6, 50°C Archaeoglobus fulgidus
2.7.1.146 5.7
-
 D-fructose 1,6-bisphosphate pH 6.6, 80°C Archaeoglobus fulgidus

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.7.1.146 soluble
-
 Archaeoglobus fulgidus
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.1.146 Co2+ 30% of activity with Mg2+ Archaeoglobus fulgidus
2.7.1.146 Fe2+ 16% of activity with Mg2+ Archaeoglobus fulgidus
2.7.1.146 Mg2+ most efficient divalent cation, can be replaced by Ni2+, Co2+, and Mn2+ Archaeoglobus fulgidus
2.7.1.146 Mn2+ 62% of activity with Mg2+ Archaeoglobus fulgidus
2.7.1.146 additional information divalent cations are required for activity, Zn2+, Ca2+, and Cu2+ are poor metal cofactors Archaeoglobus fulgidus
2.7.1.146 Ni2+ 33% of activity with Mg2+ Archaeoglobus fulgidus

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.1.146 50000
-
 2 * 50000, SDS-PAGE Archaeoglobus fulgidus
2.7.1.146 50000
-
 4 * 50000, SDS-PAGE Archaeoglobus fulgidus
2.7.1.146 100000
-
 dimeric enzyme form, gel filtration at higher protein concentration Archaeoglobus fulgidus
2.7.1.146 200000
-
 tetrameric enzyme form, gel filtration at lower protein concentration Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate Archaeoglobus fulgidus
-
 AMP + D-fructose 1,6-bisphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.1.146 Archaeoglobus fulgidus
-
 strain 7324, no activity in strain VC 16
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.7.1.146 1818fold to homogeneity by ultracentrifugation, anion exchange, ADP affinity, and cation exchange chromatography, and gel filtration Archaeoglobus fulgidus

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.1.146 12
-
 purified enzyme, determined at pH 6.6, 80°C, reverse reaction Archaeoglobus fulgidus
2.7.1.146 392
-
 purified enzyme, determined at pH 6.6, 50°C, forward reaction Archaeoglobus fulgidus

Storage Stability

EC Number Storage Stability Organism
2.7.1.146 -20°C, purified native enzyme, quite stable Archaeoglobus fulgidus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.1.146 ADP + D-fructose 6-phosphate
-
 Archaeoglobus fulgidus AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 ADP + D-fructose 6-phosphate enzyme is highly specific for D-fructose 6-phosphate in the forward reaction Archaeoglobus fulgidus AMP + D-fructose 1,6-bisphosphate
-
 r
2.7.1.146 additional information no activity with ATP, diphosphate, or acetyl phosphate as phosphate donors, no activity with D-glucose as phosphate acceptor substrate Archaeoglobus fulgidus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
2.7.1.146 dimer 2 * 50000, SDS-PAGE Archaeoglobus fulgidus
2.7.1.146 tetramer 4 * 50000, SDS-PAGE Archaeoglobus fulgidus

Synonyms

EC Number Synonyms Comment Organism
2.7.1.146 ADP-dependent 6-phosphofructokinase
-
 Archaeoglobus fulgidus
2.7.1.146 ADP-Pfk
-
 Archaeoglobus fulgidus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
2.7.1.146 90
-
-
 Archaeoglobus fulgidus

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
2.7.1.146 40 100
-
 Archaeoglobus fulgidus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
2.7.1.146 80
-
 120 min, completely stable Archaeoglobus fulgidus
2.7.1.146 100
-
 120 min, completely loss of activity after 60 min, in presence of 1 M NaCl the enzyme retains 30% activity after 90 min, in presence of 1 M KCl the enzyme retains 50% activity after 90 min Archaeoglobus fulgidus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.1.146 6.6
-
-
 Archaeoglobus fulgidus

pH Range

EC Number pH Minimum pH Maximum Comment Organism
2.7.1.146 3.9 9 50% of maximal activity at pH 6.1 and pH 7.9 Archaeoglobus fulgidus

Cofactor

EC Number Cofactor Comment Organism Structure
2.7.1.146 ADP
-
 Archaeoglobus fulgidus
2.7.1.146 additional information no activity with ATP, diphosphate, or acetyl phosphate Archaeoglobus fulgidus