EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.113 | Triton X-100 | - |
Homo sapiens |
EC Number | Application | Comment | Organism |
---|---|---|---|
2.7.1.145 | medicine | enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Bombyx mori |
2.7.1.145 | medicine | enzyme with increased specificity towards certain analogues can be used as a suicide gene in combined gene/chemotherapy treatment of cancer | Drosophila melanogaster |
2.7.1.145 | synthesis | enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used for DNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes | Drosophila melanogaster |
2.7.1.145 | synthesis | enzyme can be utilized in industrial large-scale production of dNTPs and analogues, used forDNA synthetic reactions, giving a higher yield and less toxic byproducts compared to chemical processes | Bombyx mori |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.7.1.74 | the single copy gene is located on chromosome 4q13.3-q21.1 | Homo sapiens |
2.7.1.113 | gene is localized on chromosome 2q13 | Homo sapiens |
2.7.1.145 | DNA and amino acid sequence determination and analysis, gene is located on chromosome 3 at position 91D-91E, expression of wild-type and mutant enzymes in Escherichia coli TK-deficient strain KY895 | Drosophila melanogaster |
EC Number | Crystallization (Comment) | Organism |
---|---|---|
2.7.1.113 | enzyme in complex with ATP | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
2.7.1.145 | N45D/N64D | site-directed mutagenesis, mutant shows increased drug sensitivity and decreased inhibition by dTTP compared to the wild-type enzyme | Drosophila melanogaster |
EC Number | General Stability | Organism |
---|---|---|
2.7.1.113 | Triton X-100 and ATP stabilize | Homo sapiens |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | dCTP | feedback inhibition, competitive versus phosphate donors | Homo sapiens | |
2.7.1.113 | dATP | feedback inhibition | Homo sapiens | |
2.7.1.113 | dGTP | feedback inhibition | Homo sapiens | |
2.7.1.145 | dTTP | competitive versus ATP, feedback inhibition | Bombyx mori | |
2.7.1.145 | dTTP | competitive versus ATP, feedback inhibition | Drosophila melanogaster | |
2.7.1.145 | additional information | no inhibition by dNTPs other than dTTP | Bombyx mori | |
2.7.1.145 | additional information | no inhibition by dNTPs other than dTTP | Drosophila melanogaster |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.7.1.74 | additional information | - |
additional information | no Michaelis-Menten kinetics, but negative cooperativity | Homo sapiens | |
2.7.1.145 | additional information | - |
additional information | positive cooperativity with all 4 natural deoxyribonucleosides, no hyperbolic Michaelis-Menten substrate kinetics | Bombyx mori | |
2.7.1.145 | additional information | - |
additional information | straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex | Drosophila melanogaster |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.7.1.74 | cytoplasm | - |
Homo sapiens | 5737 | - |
2.7.1.74 | nucleus | enzyme possesses a nuclear localization signal at the N-terminus | Homo sapiens | 5634 | - |
2.7.1.113 | mitochondrial matrix | - |
Homo sapiens | 5759 | - |
2.7.1.113 | mitochondrion | enzyme possesses an N-terminal mitochondrial import signal | Homo sapiens | 5739 | - |
2.7.1.113 | additional information | the enzyme leaks out into the cytosol during cell apoptosis | Homo sapiens | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | Mg2+ | - |
Homo sapiens | |
2.7.1.113 | Mg2+ | - |
Homo sapiens | |
2.7.1.145 | Mg2+ | dependent on | Bombyx mori | |
2.7.1.145 | Mg2+ | dependent on | Drosophila melanogaster |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.7.1.113 | 30000 | - |
2 * 30000 | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.74 | ATP + 2'-deoxycytidine | Homo sapiens | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + 2'-deoxycytidine | Homo sapiens | - |
UDP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2'-deoxyadenosine | Homo sapiens | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2'-deoxyguanosine | Homo sapiens | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.113 | UTP + 2'-deoxyadenosine | Homo sapiens | - |
UDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.113 | UTP + 2'-deoxyguanosine | Homo sapiens | - |
UDP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyadenosine | Drosophila melanogaster | - |
ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxycytidine | Drosophila melanogaster | - |
ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyguanosine | Drosophila melanogaster | - |
ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxythymidine | Drosophila melanogaster | - |
ADP + 2'-deoxythymidine 5'-phosphate | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.7.1.74 | Homo sapiens | P27707 | - |
- |
2.7.1.113 | Homo sapiens | Q16854 | constitutive expression | - |
2.7.1.145 | Bombyx mori | - |
- |
- |
2.7.1.145 | Drosophila melanogaster | Q9XZT6 | dNK is the only deoxyriconucleoside kinase in the fruitfly | - |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.7.1.74 | additional information | posttranscriptional regulation of the enzyme | Homo sapiens |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.7.1.113 | enzyme activity is stabilized during purification in presence of Triton X-100 and ATP | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.7.1.74 | NTP + deoxycytidine = NDP + dCMP | substrate-enzyme interactions, bi bi random reaction mechanism with ATP, but ordered with UTP binding before the acceptor substrate, substrate binding site structures | Homo sapiens | |
2.7.1.113 | ATP + deoxyguanosine = ADP + dGMP | substrate interactions, reaction mechanism | Homo sapiens | |
2.7.1.145 | ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate | positive cooperativity mechanism, substrate and nucleotide interactions and binding sites, overview | Bombyx mori | |
2.7.1.145 | ATP + a 2'-deoxyribonucleoside = ADP + a 2'-deoxyribonucleoside 5'-phosphate | straight forward hyperbolic kinetic reaction mechanism following a compulsory ordered steady-state reaction mechanism with formation of a ternary complex, substrate interactions | Drosophila melanogaster |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.7.1.74 | colonic mucosa | - |
Homo sapiens | - |
2.7.1.74 | kidney | very low enzyme expression | Homo sapiens | - |
2.7.1.74 | liver | very low enzyme expression | Homo sapiens | - |
2.7.1.74 | lymphoid tissue | high enzyme expression | Homo sapiens | - |
2.7.1.74 | additional information | tissue specific enzyme expression | Homo sapiens | - |
2.7.1.74 | muscle | very low enzyme expression | Homo sapiens | - |
2.7.1.74 | pancreas | very low enzyme expression | Homo sapiens | - |
2.7.1.113 | brain | - |
Homo sapiens | - |
2.7.1.113 | liver | - |
Homo sapiens | - |
2.7.1.113 | lymphoid tissue | - |
Homo sapiens | - |
2.7.1.113 | skin | - |
Homo sapiens | - |
2.7.1.113 | spleen | - |
Homo sapiens | - |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.7.1.74 | additional information | - |
substrate specificity | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.7.1.74 | ATP + 2',3'-dideoxycytidine | i.e. zalcitabine | Homo sapiens | ADP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2'-deoxycytidine | - |
Homo sapiens | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + 2-chloro-2'-deoxyadenosine | i.e. cladribine | Homo sapiens | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + arabinosyl adenine | - |
Homo sapiens | ADP + arabinosyl adenine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + arabinosyl cytosine | i.e. cytosar | Homo sapiens | ADP + arabinosyl cytosine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + beta-L-2',3'-dideoxy-3'-thiacytidine | - |
Homo sapiens | ADP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + difluoro-arabinofuranosyl adenine | i.e. fludarabine | Homo sapiens | ADP + difluoro-arabinofuranosyl adenine 5'-phosphate | - |
? | |
2.7.1.74 | ATP + difluorodeoxycytidine | i.e. gemcitabine | Homo sapiens | ADP + difluorodeoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | additional information | substrate-enzyme interactions, overview, enzyme prefers nucleosides with sugars in the S-conformation, i.e C2'-endo-C3'-exo, binding of substrate induces conformational changes, the phosphate donor influences the acceptor substrate specificity, mechanism, overview | Homo sapiens | ? | - |
? | |
2.7.1.74 | UTP + 2',3'-dideoxycytidine | i.e. zalcitabine | Homo sapiens | UDP + 2',3'-dideoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + 2'-deoxyadenosine | - |
Homo sapiens | UDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + 2'-deoxycytidine | - |
Homo sapiens | UDP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + 2'-deoxyguanosine | - |
Homo sapiens | UDP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + 2-chloro-2'-deoxyadenosine | i.e. cladribine | Homo sapiens | UDP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + arabinosyl adenine | - |
Homo sapiens | UDP + arabinosyl adenine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + arabinosyl cytosine | i.e. cytosar | Homo sapiens | UDP + arabinosyl cytosine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + beta-L-2',3'-dideoxy-3'-thiacytidine | - |
Homo sapiens | UDP + beta-L-2',3'-dideoxy-3'-thiacytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + beta-L-dioxolane-cytidine | antitumor drug | Homo sapiens | UDP + beta-L-dioxolane-cytidine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + difluoro-arabinofuranosyl adenine | i.e. fludarabine | Homo sapiens | UDP + difluoro-arabinofuranosyl adenine 5'-phosphate | - |
? | |
2.7.1.74 | UTP + difluorodeoxycytidine | i.e. gemcitabine | Homo sapiens | UDP + difluorodeoxycytidine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 1-beta-D-arabinofuranosyladenine | - |
Homo sapiens | ADP + 1-beta-D-arabinofuranosyladenine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2',2'-difluorodeoxyguanosine | - |
Homo sapiens | ADP + 2',2'-difluorodeoxyguanosine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2'-deoxyadenosine | - |
Homo sapiens | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2'-deoxyguanosine | - |
Homo sapiens | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2'-deoxyinosine | - |
Homo sapiens | ADP + 2'-deoxyinosine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine | - |
Homo sapiens | ADP + 2-chloro-2'-arabinofluoro-2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 2-chlorodeoxyadenosine | - |
Homo sapiens | ADP + 2-chlorodeoxyadenosine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + 9-beta-D-arabinofuranosylguanine | - |
Homo sapiens | ADP + 9-beta-D-arabinofuranosylguanine 5'-phosphate | - |
? | |
2.7.1.113 | ATP + ganciclovir | i.e. 9-(1,3-dihydroxy-2-propoxymethyl)-guanine, low activity | Homo sapiens | ADP + ganciclovir phosphate | - |
? | |
2.7.1.113 | ATP + penciclovir | i.e. 9-(2-hydroxy-1-hydroxymethyl-ethoxymethyl)-guanine, low activity | Homo sapiens | ADP + penciclovir 5'-phosphate | - |
? | |
2.7.1.113 | additional information | substrate interactions, overview, enzyme shows relaxed enantioselectivity, accepting D- and L-enantiomers of beta-deoxyguanosine but with lower activity than alpha-L-deoxyguanosine, and beta-L-deoxyadenosine, enzyme accepts purine deoxynucleosides and analogues as substrates utilizing a triphosphate donor substrate | Homo sapiens | ? | - |
? | |
2.7.1.113 | UTP + 2'-deoxyadenosine | - |
Homo sapiens | UDP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.113 | UTP + 2'-deoxyguanosine | - |
Homo sapiens | UDP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.113 | UTP + 2'-deoxyinosine | - |
Homo sapiens | UDP + 2'-deoxyinosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 1-beta-D-arabinofuranosylcytosine | - |
Drosophila melanogaster | ADP + 1-beta-D-arabinofuranosylcytosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 1-beta-D-arabinofuranosylthymine | - |
Drosophila melanogaster | ADP + 1-beta-D-arabinofuranosylthymine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxycytidine | - |
Drosophila melanogaster | ADP + 2'-deoxycytidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxyguanosine | - |
Drosophila melanogaster | ADP + 2'-deoxyguanosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2'-deoxythymidine | - |
Drosophila melanogaster | ADP + 2'-deoxythymidine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 2-chloro-2'-deoxyadenosine | - |
Drosophila melanogaster | ADP + 2-chloro-2'-deoxyadenosine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + 5-fluoro-2'-deoxyuridine | - |
Drosophila melanogaster | ADP + 5-fluoro-2'-deoxyuridine 5'-phosphate | - |
? | |
2.7.1.145 | ATP + E-5-(2-bromovinyl)-2'-deoxyuridine | - |
Drosophila melanogaster | ADP + E-5-(2-bromovinyl)-2'-deoxyuridine phosphate | - |
? | |
2.7.1.145 | additional information | broad substrate specificity | Drosophila melanogaster | ? | - |
? | |
2.7.1.145 | additional information | substrate specificity, no activity with purine ribonucleosides and dideoxynucleosides | Bombyx mori | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.7.1.74 | More | 3D-structure analysis, dCK structure model, overview | Homo sapiens |
2.7.1.113 | dimer | 2 * 30000 | Homo sapiens |
2.7.1.113 | More | ternary and quarternary structure analysis, overview | Homo sapiens |
2.7.1.145 | dimer | - |
Bombyx mori |
2.7.1.145 | dimer | - |
Drosophila melanogaster |
2.7.1.145 | More | ternary and quarternary structure analysis, overview | Bombyx mori |
2.7.1.145 | More | ternary and quarternary structure analysis, overview | Drosophila melanogaster |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.7.1.74 | dCK | - |
Homo sapiens |
2.7.1.74 | NTP:deoxycytidine 5'-phosphotransferase | - |
Homo sapiens |
2.7.1.113 | DGK | - |
Homo sapiens |
2.7.1.113 | nucleoside triphosphate: deoxyguanosine 5'-phosphotransferase | - |
Homo sapiens |
2.7.1.145 | dNK | - |
Bombyx mori |
2.7.1.145 | dNK | - |
Drosophila melanogaster |
2.7.1.145 | More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Bombyx mori |
2.7.1.145 | More | the enzyme belongs to the thymidine kinase 2-like, i.e. TK2-like, family of enzymes | Drosophila melanogaster |
2.7.1.145 | multisubstrate deoxyribonucleoside kinase | - |
Bombyx mori |
2.7.1.145 | multisubstrate deoxyribonucleoside kinase | - |
Drosophila melanogaster |
2.7.1.145 | nucleoside triphosphate: deoxyribonucleoside 5'-phosphotransferase | - |
Drosophila melanogaster |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.7.1.74 | ATP | - |
Homo sapiens | |
2.7.1.74 | additional information | enzyme lalso utilizes other nucleoside triphosphates as phosphate donors | Homo sapiens | |
2.7.1.74 | UTP | - |
Homo sapiens | |
2.7.1.113 | ATP | binding site structure and interactions, Gln111, Arg118, Met99, and Asp147 are involved, overview | Homo sapiens | |
2.7.1.113 | additional information | enzyme accepts several triphosphate donor substrates with preference for ATP and UTP | Homo sapiens | |
2.7.1.113 | UTP | - |
Homo sapiens | |
2.7.1.145 | ATP | - |
Drosophila melanogaster |