Literature summary extracted from

Temiakov, D.; Patlan, V.; Anikin, M.; McAllister, W.T.; Yokoyama, S.; Vassylyev, D.G.
Structural basis for substrate selection by T7 RNA polymerase (2004), Cell, 116, 381-391.

Crystallization (Commentary)

EC Number	Crystallization (Comment)	Organism
2.7.7.6	T7 RNAP elonation complex	Escherichia phage T7

Organism

EC Number	Organism	UniProt	Comment	Textmining
2.7.7.6	Escherichia phage T7	P00573	-	-

Substrates and Products (Substrate)

EC Number	Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
2.7.7.6	nucleoside triphosphate + RNAn	substrate specifically binds to the enzyme in the open conformation, where it is base paired with the acceptor template base, while Tyr639 provides discrimination of ribose versus deoxyribose substrates. Substrate selection occurs prior to the isomerization to the catalytically active conformation	Escherichia phage T7	diphosphate + RNAn+1	-	?

Synonyms

EC Number	Synonyms	Comment	Organism
2.7.7.6	T7 RNAP	-	Escherichia phage T7

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Release 2023.1 (January 2023)