EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
2.3.1.43 | apolipoprotein A-I | glycosylated, the alpha-helix formed by residues 143-165 is essential for full activation activity | Homo sapiens |
EC Number | Cloned (Comment) | Organism |
---|---|---|
2.3.1.43 | stable expression in CHO cells, expression in insect cells via baculovirus infection, glycosylation pattern of the recombinant enzymes differs from the wild-type, overview | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
2.3.1.43 | extracellular | enzyme is secreted into the plasma | Homo sapiens | - |
- |
EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|---|
2.3.1.43 | 47000 | - |
x * 65000-68000, glycosylated protein, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE, x * 47089, sequence calculation | Homo sapiens |
2.3.1.43 | 47089 | - |
x * 65000-68000, glycosylated protein, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE, x * 47089, sequence calculation | Homo sapiens |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.43 | phosphatidylcholine + cholesterol | Homo sapiens | isozyme abnormality cause hepatosplenic schistosomiasis mansoni, important in lipoprotein metabolism and cholesterol transport, cholesterol is trapped in the HDL particles, enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I | 3-acylglycerophosphocholine + ? | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.3.1.43 | Homo sapiens | - |
- |
- |
EC Number | Posttranslational Modification | Comment | Organism |
---|---|---|---|
2.3.1.43 | glycoprotein | analysis of glycosylation pattern, glycosylation e.g. at residues Asn20, Asn84, and Asn272, modification of the glycan side chains by use of drugs and by other enzymes, physiological function, glycosylation pattern of the recombinant enzymes differs from the wild-type, overview | Homo sapiens |
EC Number | Reaction | Comment | Organism | Reaction ID |
---|---|---|---|---|
2.3.1.43 | phosphatidylcholine + a sterol = 1-acylglycerophosphocholine + a sterol ester | reaction mechanism, His377, Asp345, and Ser181 form the catalytic triad | Homo sapiens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
2.3.1.43 | brain | - |
Homo sapiens | - |
2.3.1.43 | liver | secretion from to plasma | Homo sapiens | - |
2.3.1.43 | plasma | at least 6 plasma isozymes | Homo sapiens | - |
2.3.1.43 | testis | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.3.1.43 | additional information | the enzyme accepts a variety of acceptor substrates including sterols and several other alcohols, at high lysophosphatidylcholine concentrations the enzyme catalyzes the reverse reaction producing lecithin on LDL, i.e. exhibiting lysolecithin acyltransferase activity, in absence of an appropriate sterol acceptor, the enzyme exhibits phospholipase A2 or esterase activity liberating free fatty acids | Homo sapiens | ? | - |
? | |
2.3.1.43 | phosphatidylcholine + cholesterol | isozyme abnormality cause hepatosplenic schistosomiasis mansoni, important in lipoprotein metabolism and cholesterol transport, cholesterol is trapped in the HDL particles, enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I | Homo sapiens | 3-acylglycerophosphocholine + ? | - |
? | |
2.3.1.43 | phosphatidylcholine + cholesterol | enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I | Homo sapiens | lysolecithin + cholesteryl ester | - |
? | |
2.3.1.43 | phosphatidylcholine + cholesterol | enzyme transfers a long-chain fatty acyl residue from the sn-2 position of phosphatidylcholine, i.e. lecithin, to the 3-beta-hydroxyl group of cholesterol producing lysophosphatidylcholine or lysolecithin and cholesteryl ester, predominantly on HDL containing the activator apolipoprotein A-I | Homo sapiens | lysophosphatidylcholine + cholesteryl ester | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
2.3.1.43 | ? | x * 65000-68000, glycosylated protein, SDS-PAGE, x * 47000, deglycosylated enzyme, SDS-PAGE, x * 47089, sequence calculation | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.3.1.43 | LCAT | - |
Homo sapiens |
2.3.1.43 | lecithin-cholesterol acyltransferase | - |
Homo sapiens |
EC Number | Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|---|
2.3.1.43 | Homo sapiens | plasma isozymes | 4.8 | 3.9 |