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Literature summary extracted from

  • de Lucio, B.; Manuel, V.; Barrera-Rodriguez, R.
    Characterization of human NSCLC cell line with innate etoposide-resistance mediated by cytoplasmic localization of topoisomerase II alpha (2005), Cancer Sci., 96, 774-783.
    View publication on PubMed

Application

EC Number Application Comment Organism
5.6.2.2 medicine the isozyme alpha is a target for many chemotherapeutic agents in clinical use Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
5.6.2.2 camptothecin
-
 Homo sapiens
5.6.2.2 doxorubicin
-
 Homo sapiens
5.6.2.2 etoposide
-
 Homo sapiens
5.6.2.2 additional information cytoplasmic localization of the enzyme is a mechanism to confer drug resistance to cells, cytotoxic affects of drugs on different cell lines, overview Homo sapiens
5.6.2.2 Teniposide
-
 Homo sapiens

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
5.6.2.2 cytoplasm INER-37 cells Homo sapiens 5737
-
5.6.2.2 additional information cytoplasmic localization of the enzyme is a mechanism to confer drug resistance to cells Homo sapiens
-
-
5.6.2.2 nucleus
-
 Homo sapiens 5634
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
5.6.2.2 KCl
-
 Homo sapiens
5.6.2.2 Mg2+
-
 Homo sapiens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
5.6.2.2 65460
-
 x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE Homo sapiens
5.6.2.2 160000
-
 x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE Homo sapiens
5.6.2.2 170000
-
 x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE Homo sapiens

Organism

EC Number Organism UniProt Comment Textmining
5.6.2.2 Homo sapiens
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
5.6.2.2 HeLa cell
-
 Homo sapiens
-
5.6.2.2 INER-37 cell non-small-cell lung cancer cell line, cell line with innate etoposide-resistance mediated by cytoplasmic localization of topoisomerase II alpha, 2 enzyme forms, a large and a small one, overview Homo sapiens
-
5.6.2.2 INER-51/PSC-1 cell lung cancer cell line Homo sapiens
-
5.6.2.2 lung cancer cell line VP-1 cell Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
5.6.2.2 theta-174 phage DNA + ATP + H2O unknotting of theta-174 phage DNA Homo sapiens ?
-
 ?

Subunits

EC Number Subunits Comment Organism
5.6.2.2 ? x * 170000, HeLa cell enzyme, SDS-PAGE, x * 160000, large truncated INER-37 cell enzyme, SDS-PAGE, x * 65460, small INER-37 cell enzyme, SDS-PAGE Homo sapiens

Synonyms

EC Number Synonyms Comment Organism
5.6.2.2 topo II alpha
-
 Homo sapiens
5.6.2.2 topoisomerase II alpha
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
5.6.2.2 37
-
 DNA unknotting assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
5.6.2.2 7.5
-
 DNA unknotting assay at Homo sapiens

Cofactor

EC Number Cofactor Comment Organism Structure
5.6.2.2 ATP
-
 Homo sapiens