EC Number | Cloned (Comment) | Organism |
---|---|---|
2.6.1.42 | - |
Mycobacterium tuberculosis |
2.6.1.88 | expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells | Mycobacterium tuberculosis |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.42 | carboxymethoxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis | |
2.6.1.42 | O-(t-butyl)hydroxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis | |
2.6.1.42 | O-allylhydroxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis | |
2.6.1.42 | O-Benzylhydroxylamine | mixed-type inhibition, acts also as growth inhibitor of organism | Mycobacterium tuberculosis | |
2.6.1.88 | Canaline | about 50% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | carboxymethoxylamine | mixed-type inhibition, about 7% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | hydroxylamine-O-sulfonic acid | about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | additional information | not inhibited by O-trimethylsilylhydroxylamine | Mycobacterium tuberculosis | |
2.6.1.88 | O-allylhydroxylamine | mixed-type inhibition, about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | O-Benzylhydroxylamine | mixed-type inhibition, about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | O-ethylhydroxylamine | about 20% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | O-nitrobenzylhydroxylamine | about 30% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | O-pentafluorobenzylhydroxylamine | about 30% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | O-tert-butyldimethylsilylhydroxylamine | about 10% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | O-tert-butylhydroxylamine | mixed-type inhibition, about 7% residual activity at 1 mM | Mycobacterium tuberculosis | |
2.6.1.88 | O-tetrahydropyranylhydroxylamine | about 20% residual activity at 1 mM | Mycobacterium tuberculosis |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 1.77 | - |
L-valine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 2.5 | - |
L-leucine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 2.85 | - |
L-isoleucine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 4.2 | - |
4-methylthio-2-oxo-butanoate | cosubstrate leucine, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 5.79 | - |
L-valine | cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 6.02 | - |
L-leucine | cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 6.16 | - |
L-isoleucine | cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 6.95 | - |
2-oxoglutarate | cosubstrate L-leucine, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 7.44 | - |
L-phenylalanine | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 9.53 | - |
L-glutamate | cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 1.77 | - |
L-valine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 2.5 | - |
L-leucine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 2.85 | - |
L-isoleucine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 4.2 | - |
2-oxo-4-methylthiobutanoate | apparent Km value, with L-leucine as cosubstrate, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 7.44 | - |
L-phenylalanine | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 9.53 | - |
L-glutamate | apparent Km value, at pH 7.4, 37°C | Mycobacterium tuberculosis |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
2.6.1.42 | Mycobacterium tuberculosis | - |
- |
- |
2.6.1.88 | Mycobacterium tuberculosis | - |
- |
- |
2.6.1.88 | Mycobacterium tuberculosis H37Rv | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
2.6.1.88 | Ni2+ affinity column chromatography | Mycobacterium tuberculosis |
EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|---|
2.6.1.42 | 2.17 | - |
substrate L-phenylalanine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 2.58 | - |
substrate L-valine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 3.65 | - |
substrate L-leucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 4.22 | - |
substrate 4-methylthio-2-oxo-butanoate, cosubstrate leucine, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 4.28 | - |
substrate L-isoleucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 5.7 | - |
substrate L-glutamate, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 11.82 | - |
substrate L-valine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 12.8 | - |
substrate 2-oxoglutarate, cosubstrate L-leucine, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 13.44 | - |
substrate L-leucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis |
2.6.1.42 | 14.35 | - |
substrate L-isoleucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C | Mycobacterium tuberculosis |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
2.6.1.42 | L-glutamate + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 2-oxoglutarate + L-methionine | - |
? | |
2.6.1.42 | L-glutamate + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | 2-oxoglutarate + L-methionine | - |
? | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | - |
Mycobacterium tuberculosis | 3-methyl-2-oxopentanoate + L-glutamate | - |
? | |
2.6.1.42 | L-isoleucine + 2-oxoglutarate | - |
Mycobacterium tuberculosis H37Rv | 3-methyl-2-oxopentanoate + L-glutamate | - |
? | |
2.6.1.42 | L-isoleucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 3-methyl-2-oxopentanoate + L-methionine | - |
? | |
2.6.1.42 | L-isoleucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | 3-methyl-2-oxopentanoate + L-methionine | - |
? | |
2.6.1.42 | L-leucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 4-methyl-2-oxopentanoate + L-methionine | - |
? | |
2.6.1.42 | L-leucine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | 4-methyl-2-oxopentanoate + L-methionine | - |
? | |
2.6.1.42 | L-phenylalanine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | phenylpyruvate + L-methionine | - |
? | |
2.6.1.42 | L-phenylalanine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis H37Rv | phenylpyruvate + L-methionine | - |
? | |
2.6.1.42 | L-valine + 4-methylthio-2-oxo-butanoate | - |
Mycobacterium tuberculosis | 2-oxoisopentanoate + L-methionine | - |
? | |
2.6.1.88 | 2-oxo-4-methylthiobutanoate + an L-amino acid | isoleucine, leucine, and valine are the most effective substrates while glutamate and phenylalanine are also active as amino donors. Tyrosine and tryptophan have a much lesser ability to transaminate 2-oxo-4-methylthiobutanoate and all other L-amino acids are inactive | Mycobacterium tuberculosis | L-methionine + a 2-oxo acid | - |
? | |
2.6.1.88 | 2-oxo-4-methylthiobutanoate + L-glutamate | - |
Mycobacterium tuberculosis | L-methionine + ? | - |
? | |
2.6.1.88 | 2-oxo-4-methylthiobutanoate + L-isoleucine | - |
Mycobacterium tuberculosis | L-methionine + 2-oxo-methylvalerate | - |
? | |
2.6.1.88 | 2-oxo-4-methylthiobutanoate + L-leucine | - |
Mycobacterium tuberculosis | L-methionine + 2-oxo-isocaproate | - |
r | |
2.6.1.88 | 2-oxo-4-methylthiobutanoate + L-phenylalanine | - |
Mycobacterium tuberculosis | L-methionine + ? | - |
? | |
2.6.1.88 | 2-oxo-4-methylthiobutanoate + L-valine | - |
Mycobacterium tuberculosis | L-methionine + 2-oxo-isovalerate | - |
? | |
2.6.1.88 | L-methionine + 2-oxo-isocaproate | - |
Mycobacterium tuberculosis | 2-oxo-4-methylthiobutanoate + L-leucine | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
2.6.1.88 | BcaT | - |
Mycobacterium tuberculosis |
2.6.1.88 | branched-chain amino acid aminotransferase | - |
Mycobacterium tuberculosis |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
2.6.1.88 | pyridoxal 5'-phosphate | - |
Mycobacterium tuberculosis |
EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|---|
2.6.1.42 | 0.0082 | - |
O-Benzylhydroxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 0.011 | - |
O-(t-butyl)hydroxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 0.01424 | - |
carboxymethoxylamine | uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 0.0209 | - |
carboxymethoxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 0.0216 | - |
O-allylhydroxylamine | competitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 0.0841 | - |
O-Benzylhydroxylamine | uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 0.0856 | - |
O-(t-butyl)hydroxylamine | uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.42 | 0.2 | - |
O-allylhydroxylamine | or higher, uncompetitive component of mixed-type inhibition, pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.0082 | - |
O-Benzylhydroxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.01102 | - |
O-tert-butylhydroxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.02097 | - |
carboxymethoxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.02161 | - |
O-allylhydroxylamine | competitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.08408 | - |
O-Benzylhydroxylamine | uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.0856 | - |
O-tert-butylhydroxylamine | uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.1424 | - |
carboxymethoxylamine | uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis | |
2.6.1.88 | 0.2 | - |
O-allylhydroxylamine | Ki above 0.2 mM, uncompetitive inhibition, at pH 7.4, 37°C | Mycobacterium tuberculosis |