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Literature summary extracted from

  • Venos, E.S.; Knodel, M.H.; Radford, C.L.; Berger, B.J.
    Branched-chain amino acid aminotransferase and methionine formation in Mycobacterium tuberculosis (2004), BMC Microbiol., 4, 39.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.6.1.42
-
Mycobacterium tuberculosis
2.6.1.88 expressed in Escherichia coli BL21(DE3) CodonPlus-RIL cells Mycobacterium tuberculosis

Inhibitors

EC Number Inhibitors Comment Organism Structure
2.6.1.42 carboxymethoxylamine mixed-type inhibition, acts also as growth inhibitor of organism Mycobacterium tuberculosis
2.6.1.42 O-(t-butyl)hydroxylamine mixed-type inhibition, acts also as growth inhibitor of organism Mycobacterium tuberculosis
2.6.1.42 O-allylhydroxylamine mixed-type inhibition, acts also as growth inhibitor of organism Mycobacterium tuberculosis
2.6.1.42 O-Benzylhydroxylamine mixed-type inhibition, acts also as growth inhibitor of organism Mycobacterium tuberculosis
2.6.1.88 Canaline about 50% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 carboxymethoxylamine mixed-type inhibition, about 7% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 hydroxylamine-O-sulfonic acid about 10% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 additional information not inhibited by O-trimethylsilylhydroxylamine Mycobacterium tuberculosis
2.6.1.88 O-allylhydroxylamine mixed-type inhibition, about 10% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 O-Benzylhydroxylamine mixed-type inhibition, about 10% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 O-ethylhydroxylamine about 20% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 O-nitrobenzylhydroxylamine about 30% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 O-pentafluorobenzylhydroxylamine about 30% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 O-tert-butyldimethylsilylhydroxylamine about 10% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 O-tert-butylhydroxylamine mixed-type inhibition, about 7% residual activity at 1 mM Mycobacterium tuberculosis
2.6.1.88 O-tetrahydropyranylhydroxylamine about 20% residual activity at 1 mM Mycobacterium tuberculosis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.6.1.42 1.77
-
L-valine cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 2.5
-
L-leucine cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 2.85
-
L-isoleucine cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 4.2
-
4-methylthio-2-oxo-butanoate cosubstrate leucine, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 5.79
-
L-valine cosubstrate 2-oxoglutarate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 6.02
-
L-leucine cosubstrate 2-oxoglutarate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 6.16
-
L-isoleucine cosubstrate 2-oxoglutarate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 6.95
-
2-oxoglutarate cosubstrate L-leucine, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 7.44
-
L-phenylalanine cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 9.53
-
L-glutamate cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 1.77
-
L-valine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 2.5
-
L-leucine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 2.85
-
L-isoleucine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 4.2
-
2-oxo-4-methylthiobutanoate apparent Km value, with L-leucine as cosubstrate, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 7.44
-
L-phenylalanine apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 9.53
-
L-glutamate apparent Km value, at pH 7.4, 37°C Mycobacterium tuberculosis

Organism

EC Number Organism UniProt Comment Textmining
2.6.1.42 Mycobacterium tuberculosis
-
-
-
2.6.1.88 Mycobacterium tuberculosis
-
-
-
2.6.1.88 Mycobacterium tuberculosis H37Rv
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.6.1.88 Ni2+ affinity column chromatography Mycobacterium tuberculosis

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.6.1.42 2.17
-
substrate L-phenylalanine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 2.58
-
substrate L-valine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 3.65
-
substrate L-leucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 4.22
-
substrate 4-methylthio-2-oxo-butanoate, cosubstrate leucine, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 4.28
-
substrate L-isoleucine, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 5.7
-
substrate L-glutamate, cosubstrate 4-methylthio-2-oxo-butanoate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 11.82
-
substrate L-valine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 12.8
-
substrate 2-oxoglutarate, cosubstrate L-leucine, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 13.44
-
substrate L-leucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 14.35
-
substrate L-isoleucine, cosubstrate 2-oxoglutarate, pH 7.4, 37°C Mycobacterium tuberculosis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.6.1.42 L-glutamate + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis 2-oxoglutarate + L-methionine
-
?
2.6.1.42 L-glutamate + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis H37Rv 2-oxoglutarate + L-methionine
-
?
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Mycobacterium tuberculosis 3-methyl-2-oxopentanoate + L-glutamate
-
?
2.6.1.42 L-isoleucine + 2-oxoglutarate
-
Mycobacterium tuberculosis H37Rv 3-methyl-2-oxopentanoate + L-glutamate
-
?
2.6.1.42 L-isoleucine + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis 3-methyl-2-oxopentanoate + L-methionine
-
?
2.6.1.42 L-isoleucine + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis H37Rv 3-methyl-2-oxopentanoate + L-methionine
-
?
2.6.1.42 L-leucine + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis 4-methyl-2-oxopentanoate + L-methionine
-
?
2.6.1.42 L-leucine + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis H37Rv 4-methyl-2-oxopentanoate + L-methionine
-
?
2.6.1.42 L-phenylalanine + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis phenylpyruvate + L-methionine
-
?
2.6.1.42 L-phenylalanine + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis H37Rv phenylpyruvate + L-methionine
-
?
2.6.1.42 L-valine + 4-methylthio-2-oxo-butanoate
-
Mycobacterium tuberculosis 2-oxoisopentanoate + L-methionine
-
?
2.6.1.88 2-oxo-4-methylthiobutanoate + an L-amino acid isoleucine, leucine, and valine are the most effective substrates while glutamate and phenylalanine are also active as amino donors. Tyrosine and tryptophan have a much lesser ability to transaminate 2-oxo-4-methylthiobutanoate and all other L-amino acids are inactive Mycobacterium tuberculosis L-methionine + a 2-oxo acid
-
?
2.6.1.88 2-oxo-4-methylthiobutanoate + L-glutamate
-
Mycobacterium tuberculosis L-methionine + ?
-
?
2.6.1.88 2-oxo-4-methylthiobutanoate + L-isoleucine
-
Mycobacterium tuberculosis L-methionine + 2-oxo-methylvalerate
-
?
2.6.1.88 2-oxo-4-methylthiobutanoate + L-leucine
-
Mycobacterium tuberculosis L-methionine + 2-oxo-isocaproate
-
r
2.6.1.88 2-oxo-4-methylthiobutanoate + L-phenylalanine
-
Mycobacterium tuberculosis L-methionine + ?
-
?
2.6.1.88 2-oxo-4-methylthiobutanoate + L-valine
-
Mycobacterium tuberculosis L-methionine + 2-oxo-isovalerate
-
?
2.6.1.88 L-methionine + 2-oxo-isocaproate
-
Mycobacterium tuberculosis 2-oxo-4-methylthiobutanoate + L-leucine
-
r

Synonyms

EC Number Synonyms Comment Organism
2.6.1.88 BcaT
-
Mycobacterium tuberculosis
2.6.1.88 branched-chain amino acid aminotransferase
-
Mycobacterium tuberculosis

Cofactor

EC Number Cofactor Comment Organism Structure
2.6.1.88 pyridoxal 5'-phosphate
-
Mycobacterium tuberculosis

Ki Value [mM]

EC Number Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
2.6.1.42 0.0082
-
O-Benzylhydroxylamine competitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 0.011
-
O-(t-butyl)hydroxylamine competitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 0.01424
-
carboxymethoxylamine uncompetitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 0.0209
-
carboxymethoxylamine competitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 0.0216
-
O-allylhydroxylamine competitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 0.0841
-
O-Benzylhydroxylamine uncompetitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 0.0856
-
O-(t-butyl)hydroxylamine uncompetitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.42 0.2
-
O-allylhydroxylamine or higher, uncompetitive component of mixed-type inhibition, pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.0082
-
O-Benzylhydroxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.01102
-
O-tert-butylhydroxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.02097
-
carboxymethoxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.02161
-
O-allylhydroxylamine competitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.08408
-
O-Benzylhydroxylamine uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.0856
-
O-tert-butylhydroxylamine uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.1424
-
carboxymethoxylamine uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis
2.6.1.88 0.2
-
O-allylhydroxylamine Ki above 0.2 mM, uncompetitive inhibition, at pH 7.4, 37°C Mycobacterium tuberculosis