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Literature summary extracted from

  • Bao, J.; Ryu, D.D.
    Cloning of deoxynucleoside monophosphate kinase genes and biosynthesis of deoxynucleoside diphosphates (2006), Biotechnol. Bioeng., 93, 572-580.
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
2.7.4.13 expression in Escherichia coli strain BL21 (DE3) Saccharomyces cerevisiae
2.7.4.13 expression in Escherichia coli strain BL21 (DE3), CK expression at 37°C forms insoluble inclusion bodoes, therefore expression was performed at 25°C Saccharomyces cerevisiae

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
2.7.4.13 0.11
-
 ATP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae
2.7.4.13 0.28
-
 ATP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae
2.7.4.13 0.33
-
 dGMP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae
2.7.4.13 0.48
-
 ATP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae
2.7.4.13 0.53
-
 dCMP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae
2.7.4.13 0.67
-
 ATP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae
2.7.4.13 0.89
-
 dTMP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae
2.7.4.13 1.1
-
 dAMP at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations Saccharomyces cerevisiae

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
2.7.4.13 Mg2+ dAMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dAMP to the corresponding active sites Saccharomyces cerevisiae
2.7.4.13 Mg2+ dCMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dCMP to the corresponding active sites Saccharomyces cerevisiae
2.7.4.13 Mg2+ dGMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dGMP to the corresponding active sites Saccharomyces cerevisiae
2.7.4.13 Mg2+ dTMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dTMP to the corresponding active sites Saccharomyces cerevisiae

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
2.7.4.13 20640
-
 calculated from amino acid sequence Saccharomyces cerevisiae
2.7.4.13 22930
-
 calculated from amino acid sequence Saccharomyces cerevisiae
2.7.4.13 24260
-
 calculated from amino acid sequence Saccharomyces cerevisiae
2.7.4.13 24690
-
 calculated from amino acid sequence Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
2.7.4.13 ATP + deoxynucleoside monophosphate Saccharomyces cerevisiae key step in de novo DNA precursor biosynthesis ADP + deoxynucleoside diphosphate
-
 r

Organism

EC Number Organism UniProt Comment Textmining
2.7.4.13 Saccharomyces cerevisiae
-
 ATCC 2610
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
2.7.4.13 20
-
-
 Saccharomyces cerevisiae
2.7.4.13 30
-
-
 Saccharomyces cerevisiae
2.7.4.13 80
-
-
 Saccharomyces cerevisiae
2.7.4.13 100
-
-
 Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.7.4.13 ATP + dAMP
-
 Saccharomyces cerevisiae ADP + dADP
-
 r
2.7.4.13 ATP + dCMP
-
 Saccharomyces cerevisiae ADP + dCDP
-
 r
2.7.4.13 ATP + deoxynucleoside monophosphate key step in de novo DNA precursor biosynthesis Saccharomyces cerevisiae ADP + deoxynucleoside diphosphate
-
 r
2.7.4.13 ATP + dGMP
-
 Saccharomyces cerevisiae ADP + dGDP
-
 r
2.7.4.13 ATP + dTMP
-
 Saccharomyces cerevisiae ADP + dTDP
-
 r

Synonyms

EC Number Synonyms Comment Organism
2.7.4.13 AK
-
 Saccharomyces cerevisiae
2.7.4.13 CK
-
 Saccharomyces cerevisiae
2.7.4.13 deoxyadenylate monophosphate kinase
-
 Saccharomyces cerevisiae
2.7.4.13 deoxycytidylate monophosphate kinase
-
 Saccharomyces cerevisiae
2.7.4.13 deoxyguanylate monophosphate kinase
-
 Saccharomyces cerevisiae
2.7.4.13 deoxynucleoside monophosphate kinase
-
 Saccharomyces cerevisiae
2.7.4.13 deoxythymidylate monophosphate kinase
-
 Saccharomyces cerevisiae
2.7.4.13 dNMP kinase
-
 Saccharomyces cerevisiae
2.7.4.13 GK
-
 Saccharomyces cerevisiae
2.7.4.13 TK
-
 Saccharomyces cerevisiae

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
2.7.4.13 additional information
-
 between pH 7-8 Saccharomyces cerevisiae
2.7.4.13 8
-
-
 Saccharomyces cerevisiae