BRENDA - Enzyme Database

Cloning of deoxynucleoside monophosphate kinase genes and biosynthesis of deoxynucleoside diphosphates

Bao, J.; Ryu, D.D.; Biotechnol. Bioeng. 93, 572-580 (2006)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
2.7.4.13
expression in Escherichia coli strain BL21 (DE3); expression in Escherichia coli strain BL21 (DE3), CK expression at 37°C forms insoluble inclusion bodoes, therefore expression was performed at 25°C
Saccharomyces cerevisiae
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.13
0.11
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.28
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.33
-
dGMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.48
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.53
-
dCMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.67
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.89
-
dTMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
1.1
-
dAMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.13
Mg2+
dAMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dAMP to the corresponding active sites; dCMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dCMP to the corresponding active sites; dGMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dGMP to the corresponding active sites; dTMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dTMP to the corresponding active sites
Saccharomyces cerevisiae
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.13
20640
-
calculated from amino acid sequence
Saccharomyces cerevisiae
2.7.4.13
22930
-
calculated from amino acid sequence
Saccharomyces cerevisiae
2.7.4.13
24260
-
calculated from amino acid sequence
Saccharomyces cerevisiae
2.7.4.13
24690
-
calculated from amino acid sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside monophosphate
Saccharomyces cerevisiae
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
r
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
2.7.4.13
Saccharomyces cerevisiae
-
ATCC 2610
-
Specific Activity [micromol/min/mg]
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.13
20
-
-
Saccharomyces cerevisiae
2.7.4.13
30
-
-
Saccharomyces cerevisiae
2.7.4.13
80
-
-
Saccharomyces cerevisiae
2.7.4.13
100
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + dAMP
-
661371
Saccharomyces cerevisiae
ADP + dADP
-
-
-
r
2.7.4.13
ATP + dCMP
-
661371
Saccharomyces cerevisiae
ADP + dCDP
-
-
-
r
2.7.4.13
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
661371
Saccharomyces cerevisiae
ADP + deoxynucleoside diphosphate
-
-
-
r
2.7.4.13
ATP + dGMP
-
661371
Saccharomyces cerevisiae
ADP + dGDP
-
-
-
r
2.7.4.13
ATP + dTMP
-
661371
Saccharomyces cerevisiae
ADP + dTDP
-
-
-
r
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.13
additional information
-
between pH 7-8
Saccharomyces cerevisiae
2.7.4.13
8
-
-
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
2.7.4.13
expression in Escherichia coli strain BL21 (DE3); expression in Escherichia coli strain BL21 (DE3), CK expression at 37°C forms insoluble inclusion bodoes, therefore expression was performed at 25°C
Saccharomyces cerevisiae
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
2.7.4.13
0.11
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.28
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.33
-
dGMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.48
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.53
-
dCMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.67
-
ATP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
0.89
-
dTMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
2.7.4.13
1.1
-
dAMP
at 30°C, pH 8.0 and saturating Mg2+ and K+ concentrations
Saccharomyces cerevisiae
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
2.7.4.13
Mg2+
dAMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dAMP to the corresponding active sites; dCMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dCMP to the corresponding active sites; dGMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dGMP to the corresponding active sites; dTMP reacts only with ATP after ATP forms the bivalent metal-ion complex MgATP2-, Mg2+ orients and directs ATP and dTMP to the corresponding active sites
Saccharomyces cerevisiae
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
2.7.4.13
20640
-
calculated from amino acid sequence
Saccharomyces cerevisiae
2.7.4.13
22930
-
calculated from amino acid sequence
Saccharomyces cerevisiae
2.7.4.13
24260
-
calculated from amino acid sequence
Saccharomyces cerevisiae
2.7.4.13
24690
-
calculated from amino acid sequence
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2.7.4.13
ATP + deoxynucleoside monophosphate
Saccharomyces cerevisiae
key step in de novo DNA precursor biosynthesis
ADP + deoxynucleoside diphosphate
-
-
r
Specific Activity [micromol/min/mg] (protein specific)
EC Number
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
2.7.4.13
20
-
-
Saccharomyces cerevisiae
2.7.4.13
30
-
-
Saccharomyces cerevisiae
2.7.4.13
80
-
-
Saccharomyces cerevisiae
2.7.4.13
100
-
-
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2.7.4.13
ATP + dAMP
-
661371
Saccharomyces cerevisiae
ADP + dADP
-
-
-
r
2.7.4.13
ATP + dCMP
-
661371
Saccharomyces cerevisiae
ADP + dCDP
-
-
-
r
2.7.4.13
ATP + deoxynucleoside monophosphate
key step in de novo DNA precursor biosynthesis
661371
Saccharomyces cerevisiae
ADP + deoxynucleoside diphosphate
-
-
-
r
2.7.4.13
ATP + dGMP
-
661371
Saccharomyces cerevisiae
ADP + dGDP
-
-
-
r
2.7.4.13
ATP + dTMP
-
661371
Saccharomyces cerevisiae
ADP + dTDP
-
-
-
r
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
2.7.4.13
additional information
-
between pH 7-8
Saccharomyces cerevisiae
2.7.4.13
8
-
-
Saccharomyces cerevisiae